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Article

Deoxycytidine kinase participates in the regulation of radiation-induced autophagy and apoptosis in breast cancer cells

  • Authors:
    • Rui Zhong
    • Bing Liang
    • Rui Xin
    • Xuanji Zhu
    • Zhuo Liu
    • Qiao Chen
    • Yufei Hou
    • Zhao Jin
    • Mu Qi
    • Shumei Ma
    • Xiaodong Liu
  • View Affiliations / Copyright

    Affiliations: Cancer Translational Medicine Laboratory, Jilin Provincial Cancer Hospital, Changchun, Jilin 130012, P.R. China, Key Laboratory of Radiobiology (Ministry of Health), School of Public Health, Jilin University, Changchun, Jilin 130021, P.R. China, Medical Records Room, The First Hospital Affiliated to Jilin University, Changchun, Jilin 130021, P.R. China, School of Public Health and Management, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China
  • Pages: 1000-1010
    |
    Published online on: January 22, 2018
       https://doi.org/10.3892/ijo.2018.4250
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Abstract

Deoxycytidine kinase (dCK) is a rate limiting enzyme critical for the phosphorylation of endogenous deoxynucleosides and for the anti‑tumor activity of many nucleoside analogs. dCK is activated in response to ionizing radiation (IR) and it is required for the G2/M checkpoint induced by IR. However, whether dCK plays a role in radiation-induced autophagy and apoptosis is less clear. In this study, we reported that dCK decreased IR-induced total cell death and apoptosis, and increased IR-induced autophagy in SKBR3 and MDA‑MB‑231 breast cancer cell lines. A molecular switch exists between apoptosis and autophagy. We further demonstrated that serine 74 phosphorylation was required for the regulation of autophagy. In dCK wild‑type (WT) or dCK S74E (mutant) MDA‑MB‑231 cell models, the expression levels of phospho-Akt, phospho-mammalian target of rapamycin (mTOR) and phospho-P70S6K significantly decreased following exposure to IR. Moreover, the ratio of Bcl‑2/Beclin1 (BECN1) significantly decreased in the S74E mutant cells; however, no change was observed in the ratio of Bcl‑2/BAX. Taken together, our findings indicate that phosphorylated and activated dCK inhibits IR-induced total cell death and apoptosis, and promotes IR-induced autophagy through the mTOR pathway and by inhibiting the binding of Bcl‑2 protein to BECN1.
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Copy and paste a formatted citation
Spandidos Publications style
Zhong R, Liang B, Xin R, Zhu X, Liu Z, Chen Q, Hou Y, Jin Z, Qi M, Ma S, Ma S, et al: Deoxycytidine kinase participates in the regulation of radiation-induced autophagy and apoptosis in breast cancer cells. Int J Oncol 52: 1000-1010, 2018.
APA
Zhong, R., Liang, B., Xin, R., Zhu, X., Liu, Z., Chen, Q. ... Liu, X. (2018). Deoxycytidine kinase participates in the regulation of radiation-induced autophagy and apoptosis in breast cancer cells. International Journal of Oncology, 52, 1000-1010. https://doi.org/10.3892/ijo.2018.4250
MLA
Zhong, R., Liang, B., Xin, R., Zhu, X., Liu, Z., Chen, Q., Hou, Y., Jin, Z., Qi, M., Ma, S., Liu, X."Deoxycytidine kinase participates in the regulation of radiation-induced autophagy and apoptosis in breast cancer cells". International Journal of Oncology 52.3 (2018): 1000-1010.
Chicago
Zhong, R., Liang, B., Xin, R., Zhu, X., Liu, Z., Chen, Q., Hou, Y., Jin, Z., Qi, M., Ma, S., Liu, X."Deoxycytidine kinase participates in the regulation of radiation-induced autophagy and apoptosis in breast cancer cells". International Journal of Oncology 52, no. 3 (2018): 1000-1010. https://doi.org/10.3892/ijo.2018.4250
Copy and paste a formatted citation
x
Spandidos Publications style
Zhong R, Liang B, Xin R, Zhu X, Liu Z, Chen Q, Hou Y, Jin Z, Qi M, Ma S, Ma S, et al: Deoxycytidine kinase participates in the regulation of radiation-induced autophagy and apoptosis in breast cancer cells. Int J Oncol 52: 1000-1010, 2018.
APA
Zhong, R., Liang, B., Xin, R., Zhu, X., Liu, Z., Chen, Q. ... Liu, X. (2018). Deoxycytidine kinase participates in the regulation of radiation-induced autophagy and apoptosis in breast cancer cells. International Journal of Oncology, 52, 1000-1010. https://doi.org/10.3892/ijo.2018.4250
MLA
Zhong, R., Liang, B., Xin, R., Zhu, X., Liu, Z., Chen, Q., Hou, Y., Jin, Z., Qi, M., Ma, S., Liu, X."Deoxycytidine kinase participates in the regulation of radiation-induced autophagy and apoptosis in breast cancer cells". International Journal of Oncology 52.3 (2018): 1000-1010.
Chicago
Zhong, R., Liang, B., Xin, R., Zhu, X., Liu, Z., Chen, Q., Hou, Y., Jin, Z., Qi, M., Ma, S., Liu, X."Deoxycytidine kinase participates in the regulation of radiation-induced autophagy and apoptosis in breast cancer cells". International Journal of Oncology 52, no. 3 (2018): 1000-1010. https://doi.org/10.3892/ijo.2018.4250
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