PFKFB3 is involved in breast cancer proliferation, migration, invasion and angiogenesis

  • Authors:
    • Fang Peng
    • Qiang Li
    • Jia-Yuan Sun
    • Ying Luo
    • Ming Chen
    • Yong Bao
  • View Affiliations

  • Published online on: January 30, 2018     https://doi.org/10.3892/ijo.2018.4257
  • Pages: 945-954
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Abstract

6-Phosphofructo 2-kinase/fructose 2, 6-bisphosphatase 3 (PFKFB3) has been reported to be overexpressed in human cancer tissues and to promote the proliferation and migration of cancer cells. However, the role of PFKFB3 in the progression and prognosis of breast cancer is not yet fully understood. In the present study, we investigated the specific role of PFKFB3 in breast cancer progression and its preliminary mechanisms of action. We first used an immunohistochemistry assay to determine that PFKFB3 was highly expressed in breast cancer tissues and that this high level of expression was involved in the poor overall survival of patients with breast cancer. In addition, the suppression of PFKFB3 by lentiviruses carrying shRNA against PFKFB3 (shPFKFB3) subsequently inhibited breast cancer cell (MDA-MB-231 and MDA-MB-468) proliferation, migration and invasion, and induced cell cycle G1 and S phase arrest in vitro. Moreover, PFKFB3 inhibition decreased p-AKT and increased p27 expression levels in breast cancer cells. Furthermore, PFKFB3 suppression inhibited breast cancer cell tumor xenograft growth in nude mice. We also found that PFKFB3 inhibition suppressed vascular endothelial growth factor α (VEGFα) protein expression and inhibited the angiogenic activity of human umbilical vein endothelial cells (HUVECs). On the whole, our results indicate that PFKFB3 is involved in the proliferation, migration, invasion and angiogenesis of breast cancer.

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March 2018
Volume 52 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Peng, F., Li, Q., Sun, J., Luo, Y., Chen, M., & Bao, Y. (2018). PFKFB3 is involved in breast cancer proliferation, migration, invasion and angiogenesis. International Journal of Oncology, 52, 945-954. https://doi.org/10.3892/ijo.2018.4257
MLA
Peng, F., Li, Q., Sun, J., Luo, Y., Chen, M., Bao, Y."PFKFB3 is involved in breast cancer proliferation, migration, invasion and angiogenesis". International Journal of Oncology 52.3 (2018): 945-954.
Chicago
Peng, F., Li, Q., Sun, J., Luo, Y., Chen, M., Bao, Y."PFKFB3 is involved in breast cancer proliferation, migration, invasion and angiogenesis". International Journal of Oncology 52, no. 3 (2018): 945-954. https://doi.org/10.3892/ijo.2018.4257