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Article

PFKFB3 is involved in breast cancer proliferation, migration, invasion and angiogenesis

  • Authors:
    • Fang Peng
    • Qiang Li
    • Jia-Yuan Sun
    • Ying Luo
    • Ming Chen
    • Yong Bao
  • View Affiliations / Copyright

    Affiliations: Department of Radiation Oncology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510080, P.R. China, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Department of Radiation Oncology, Collaborative Innovation Center of Cancer Medicine, Guangzhou, Guangdong 510060, P.R. China, Department of Clinical Laboratory, Guangdong General Hospital and Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510080, P.R. China, Department of Radiation Oncology, Zhejiang Cancer Hospital, Zhejiang Key Laboratory of Radiation Oncology, Hangzhou, Zhejiang 310022, P.R. China
  • Pages: 945-954
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    Published online on: January 30, 2018
       https://doi.org/10.3892/ijo.2018.4257
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Abstract

6-Phosphofructo 2-kinase/fructose 2, 6-bisphosphatase 3 (PFKFB3) has been reported to be overexpressed in human cancer tissues and to promote the proliferation and migration of cancer cells. However, the role of PFKFB3 in the progression and prognosis of breast cancer is not yet fully understood. In the present study, we investigated the specific role of PFKFB3 in breast cancer progression and its preliminary mechanisms of action. We first used an immunohistochemistry assay to determine that PFKFB3 was highly expressed in breast cancer tissues and that this high level of expression was involved in the poor overall survival of patients with breast cancer. In addition, the suppression of PFKFB3 by lentiviruses carrying shRNA against PFKFB3 (shPFKFB3) subsequently inhibited breast cancer cell (MDA-MB-231 and MDA-MB-468) proliferation, migration and invasion, and induced cell cycle G1 and S phase arrest in vitro. Moreover, PFKFB3 inhibition decreased p-AKT and increased p27 expression levels in breast cancer cells. Furthermore, PFKFB3 suppression inhibited breast cancer cell tumor xenograft growth in nude mice. We also found that PFKFB3 inhibition suppressed vascular endothelial growth factor α (VEGFα) protein expression and inhibited the angiogenic activity of human umbilical vein endothelial cells (HUVECs). On the whole, our results indicate that PFKFB3 is involved in the proliferation, migration, invasion and angiogenesis of breast cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Peng F, Li Q, Sun J, Luo Y, Chen M and Bao Y: PFKFB3 is involved in breast cancer proliferation, migration, invasion and angiogenesis. Int J Oncol 52: 945-954, 2018.
APA
Peng, F., Li, Q., Sun, J., Luo, Y., Chen, M., & Bao, Y. (2018). PFKFB3 is involved in breast cancer proliferation, migration, invasion and angiogenesis. International Journal of Oncology, 52, 945-954. https://doi.org/10.3892/ijo.2018.4257
MLA
Peng, F., Li, Q., Sun, J., Luo, Y., Chen, M., Bao, Y."PFKFB3 is involved in breast cancer proliferation, migration, invasion and angiogenesis". International Journal of Oncology 52.3 (2018): 945-954.
Chicago
Peng, F., Li, Q., Sun, J., Luo, Y., Chen, M., Bao, Y."PFKFB3 is involved in breast cancer proliferation, migration, invasion and angiogenesis". International Journal of Oncology 52, no. 3 (2018): 945-954. https://doi.org/10.3892/ijo.2018.4257
Copy and paste a formatted citation
x
Spandidos Publications style
Peng F, Li Q, Sun J, Luo Y, Chen M and Bao Y: PFKFB3 is involved in breast cancer proliferation, migration, invasion and angiogenesis. Int J Oncol 52: 945-954, 2018.
APA
Peng, F., Li, Q., Sun, J., Luo, Y., Chen, M., & Bao, Y. (2018). PFKFB3 is involved in breast cancer proliferation, migration, invasion and angiogenesis. International Journal of Oncology, 52, 945-954. https://doi.org/10.3892/ijo.2018.4257
MLA
Peng, F., Li, Q., Sun, J., Luo, Y., Chen, M., Bao, Y."PFKFB3 is involved in breast cancer proliferation, migration, invasion and angiogenesis". International Journal of Oncology 52.3 (2018): 945-954.
Chicago
Peng, F., Li, Q., Sun, J., Luo, Y., Chen, M., Bao, Y."PFKFB3 is involved in breast cancer proliferation, migration, invasion and angiogenesis". International Journal of Oncology 52, no. 3 (2018): 945-954. https://doi.org/10.3892/ijo.2018.4257
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