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International Journal of Oncology
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Article

Downregulation of SOX3 leads to the inhibition of the proliferation, migration and invasion of osteosarcoma cells

  • Authors:
    • Yanjie Guo
    • Jimin Yin
    • Mingjie Tang
    • Xingang Yu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, P.R. China
  • Pages: 1277-1284
    |
    Published online on: February 20, 2018
       https://doi.org/10.3892/ijo.2018.4278
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Abstract

Sex determining region Y-box protein 3 (SOX3) is involved in embryonic development and tumorigenesis. However, the expression and precise role of SOX3 in osteosarcoma remain unclear. In this study, we reported that SOX3 expression was upregulated in osteosarcoma tissues compared with non-cancerous bone cyst tissues. To elucidate the cellular and molecular function of SOX3, we examined the consequences of SOX3 knockdown in osteosarcoma cells. We found that the downregulation of SOX3 inhibited the proliferation, migration and invasion of osteosarcoma cells. SOX3 downregulation also increased the cell population in the G1 phase and induced cell apoptosis. SOX3 knockdown-mediated cell cycle arrest and cell apoptosis were associated with decreased levels of Cdc25A, cyclin D1, proliferating cell nuclear antigen (PCNA) and Bcl-2, as well as an increased Bax expression. We also found that the downregulation of SOX3 decreased the expression of Snail, Twist and matrix metalloproteinase-9 (MMP-9), and increased E-cadherin expression, resulting in the inhibition of cell migration and invasion. Taken together, our data indicate that SOX3 may serve as an oncogene in osteosarcoma, and SOX3 downregulation may prove to be a novel approach for the inhibition of osteosarcoma progression.
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1 

Rabinowicz R, Barchana M, Liphshiz I, Futerman B, Linn S and Weyl-Ben-Arush M: Cancer incidence and survival among children and adolescents in Israel during the years 1998 to 2007. J Pediatr Hematol Oncol. 34:421–429. 2012. View Article : Google Scholar : PubMed/NCBI

2 

Mirabello L, Troisi RJ and Savage SA: Osteosarcoma incidence and survival rates from 1973 to 2004: Data from the Surveillance, Epidemiology, and End Results Program. Cancer. 115:1531–1543. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Ferguson WS and Goorin AM: Current treatment of osteosarcoma. Cancer Invest. 19:292–315. 2001. View Article : Google Scholar : PubMed/NCBI

4 

Sarkar A and Hochedlinger K: The sox family of transcription factors: Versatile regulators of stem and progenitor cell fate. Cell Stem Cell. 12:15–30. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Alatzoglou KS, Azriyanti A, Rogers N, Ryan F, Curry N, Noakes C, Bignell P, Hall GW, Littooij AS, Saunders D, et al: SOX3 deletion in mouse and human is associated with persistence of the craniopharyngeal canal. J Clin Endocrinol Metab. 99:E2702–E2708. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Archer TC, Jin J and Casey ES: Interaction of Sox1, Sox2, Sox3 and Oct4 during primary neurogenesis. Dev Biol. 350:429–440. 2011. View Article : Google Scholar :

7 

Acloque H, Ocaña OH, Matheu A, Rizzoti K, Wise C, Lovell-Badge R and Nieto MA: Reciprocal repression between Sox3 and snail transcription factors defines embryonic territories at gastrulation. Dev Cell. 21:546–558. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Xia Y, Papalopulu N, Vogt PK and Li J: The oncogenic potential of the high mobility group box protein Sox3. Cancer Res. 60:6303–6306. 2000.PubMed/NCBI

9 

Yang J, Yuan D, Li J, Zheng S and Wang B: miR-186 downregulates protein phosphatase PPM1B in bladder cancer and mediates G1-S phase transition. Tumour Biol. 37:4331–4341. 2016. View Article : Google Scholar

10 

Cai QY, Liang GY, Zheng YF, Tan QY, Wang RW and Li K: Sox3 silencing inhibits metastasis and growth of esophageal squamous cell carcinoma cell via Downregulating GSK-3β. Int J Clin Exp Pathol. 9:2939–2949. 2016.

11 

Basu-Roy U, Seo E, Ramanathapuram L, Rapp TB, Perry JA, Orkin SH, Mansukhani A and Basilico C: Sox2 maintains self renewal of tumor-initiating cells in osteosarcomas. Oncogene. 31:2270–2282. 2012. View Article : Google Scholar

12 

Yang C, Hou C, Zhang H, Wang D, Ma Y, Zhang Y, Xu X, Bi Z and Geng S: miR-126 functions as a tumor suppressor in osteosarcoma by targeting Sox2. Int J Mol Sci. 15:423–437. 2013. View Article : Google Scholar

13 

Zhu H, Tang J, Tang M and Cai H: Upregulation of SOX9 in osteosarcoma and its association with tumor progression and patients' prognosis. Diagn Pathol. 8:1832013. View Article : Google Scholar : PubMed/NCBI

14 

Wu Z, Liu J, Wang J and Zhang F: SOX18 knockdown suppresses the proliferation and metastasis, and induces the apoptosis of osteosarcoma cells. Mol Med Rep. 13:497–504. 2016. View Article : Google Scholar

15 

Oltval ZN, Milliman CL and Korsmeyer SJ: Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programed cell death. Cell. 74:609–619. 1993. View Article : Google Scholar

16 

Yang J and Weinberg RA: Epithelial-mesenchymal transition: At the crossroads of development and tumor metastasis. Dev Cell. 14:818–829. 2008. View Article : Google Scholar : PubMed/NCBI

17 

Huber MA, Kraut N and Beug H: Molecular requirements for epithelial-mesenchymal transition during tumor progression. Curr Opin Cell Biol. 17:548–558. 2005. View Article : Google Scholar : PubMed/NCBI

18 

Li K, Wang RW, Jiang YG, Zou YB and Guo W: Overexpression of Sox3 is associated with diminished prognosis in esophageal squamous cell carcinoma. Ann Surg Oncol. 20(Suppl 3): S459–S466. 2013. View Article : Google Scholar

19 

Evan GI and Vousden KH: Proliferation, cell cycle and apoptosis in cancer. Nature. 411:342–348. 2001. View Article : Google Scholar : PubMed/NCBI

20 

Otsubo T, Akiyama Y, Yanagihara K and Yuasa Y: SOX2 is frequently downregulated in gastric cancers and inhibits cell growth through cell-cycle arrest and apoptosis. Br J Cancer. 98:824–831. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Zhang Y, Huang S, Dong W, Li L, Feng Y, Pan L, Han Z, Wang X, Ren G, Su D, et al: SOX7, downregulated in colorectal cancer, induces apoptosis and inhibits proliferation of colorectal cancer cells. Cancer Lett. 277:29–37. 2009. View Article : Google Scholar

22 

Ferletta M, Caglayan D, Mokvist L, Jiang Y, Kastemar M, Uhrbom L and Westermark B: Forced expression of Sox21 inhibits Sox2 and induces apoptosis in human glioma cells. Int J Cancer. 129:45–60. 2011. View Article : Google Scholar

23 

Lee RJ, Albanese C, Stenger RJ, Watanabe G, Inghirami G, Haines GK III, Webster M, Muller WJ, Brugge JS, Davis RJ, et al: pp60(v-src) induction of cyclin D1 requires collaborative interactions between the extracellular signal-regulated kinase, p38, and Jun kinase pathways. A role for cAMP response element-binding protein and activating transcription factor-2 in pp60(v-src) signaling in breast cancer cells. J Biol Chem. 274:7341–7350. 1999. View Article : Google Scholar : PubMed/NCBI

24 

Shtutman M, Zhurinsky J, Simcha I, Albanese C, D'Amico M, Pestell R and Ben-Ze'ev A: The cyclin D1 gene is a target of the beta-catenin/LEF-1 pathway. Proc Natl Acad Sci USA. 96:5522–5527. 1999. View Article : Google Scholar : PubMed/NCBI

25 

Vijayakumar S, Liu G, Rus IA, Yao S, Chen Y, Akiri G, Grumolato L and Aaronson SA: High-frequency canonical Wnt activation in multiple sarcoma subtypes drives proliferation through a TCF/β-catenin target gene, CDC25A. Cancer Cell. 19:601–612. 2011. View Article : Google Scholar : PubMed/NCBI

26 

Kormish JD, Sinner D and Zorn AM: Interactions between SOX factors and Wnt/β-catenin signaling in development and disease. Dev Dyn. 239:56–68. 2010.

27 

Zorn AM, Barish GD, Williams BO, Lavender P, Klymkowsky MW and Varmus HE: Regulation of Wnt signaling by Sox proteins: XSox17 alpha/beta and XSox3 physically interact with beta-catenin. Mol Cell. 4:487–498. 1999. View Article : Google Scholar : PubMed/NCBI

28 

Li X, Xu Y, Chen Y, Chen S, Jia X, Sun T, Liu Y, Li X, Xiang R and Li N: SOX2 promotes tumor metastasis by stimulating epithelial-to-mesenchymal transition via regulation of WNT/β-catenin signal network. Cancer Lett. 336:379–389. 2013. View Article : Google Scholar : PubMed/NCBI

29 

Yang N, Hui L, Wang Y, Yang H and Jiang X: SOX2 promotes the migration and invasion of laryngeal cancer cells by induction of MMP-2 via the PI3K/Akt/mTOR pathway. Oncol Rep. 31:2651–2659. 2014. View Article : Google Scholar : PubMed/NCBI

30 

Yang N, Hui L, Wang Y, Yang H and Jiang X: Overexpression of SOX2 promotes migration, invasion, and epithelial-mesenchymal transition through the Wnt/β-catenin pathway in laryngeal cancer Hep-2 cells. Tumour Biol. 35:7965–7973. 2014. View Article : Google Scholar : PubMed/NCBI

31 

Jafarnejad SM, Wani AA, Martinka M and Li G: Prognostic significance of Sox4 expression in human cutaneous melanoma and its role in cell migration and invasion. Am J Pathol. 177:2741–2752. 2010. View Article : Google Scholar : PubMed/NCBI

32 

Parvani JG and Schiemann WP: Sox4, EMT programs, and the metastatic progression of breast cancers: Mastering the masters of EMT. Breast Cancer Res. 15:R722013. View Article : Google Scholar : PubMed/NCBI

33 

Guan Z, Zhang J, Wang J, Wang H, Zheng F, Peng J, Xu Y, Yan M, Liu B, Cui B, et al: SOX1 Downregulates β-catenin and reverses malignant phenotype in nasopharyngeal carcinoma. Mol Cancer. 13:2572014. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Guo Y, Yin J, Tang M and Yu X: Downregulation of SOX3 leads to the inhibition of the proliferation, migration and invasion of osteosarcoma cells. Int J Oncol 52: 1277-1284, 2018.
APA
Guo, Y., Yin, J., Tang, M., & Yu, X. (2018). Downregulation of SOX3 leads to the inhibition of the proliferation, migration and invasion of osteosarcoma cells. International Journal of Oncology, 52, 1277-1284. https://doi.org/10.3892/ijo.2018.4278
MLA
Guo, Y., Yin, J., Tang, M., Yu, X."Downregulation of SOX3 leads to the inhibition of the proliferation, migration and invasion of osteosarcoma cells". International Journal of Oncology 52.4 (2018): 1277-1284.
Chicago
Guo, Y., Yin, J., Tang, M., Yu, X."Downregulation of SOX3 leads to the inhibition of the proliferation, migration and invasion of osteosarcoma cells". International Journal of Oncology 52, no. 4 (2018): 1277-1284. https://doi.org/10.3892/ijo.2018.4278
Copy and paste a formatted citation
x
Spandidos Publications style
Guo Y, Yin J, Tang M and Yu X: Downregulation of SOX3 leads to the inhibition of the proliferation, migration and invasion of osteosarcoma cells. Int J Oncol 52: 1277-1284, 2018.
APA
Guo, Y., Yin, J., Tang, M., & Yu, X. (2018). Downregulation of SOX3 leads to the inhibition of the proliferation, migration and invasion of osteosarcoma cells. International Journal of Oncology, 52, 1277-1284. https://doi.org/10.3892/ijo.2018.4278
MLA
Guo, Y., Yin, J., Tang, M., Yu, X."Downregulation of SOX3 leads to the inhibition of the proliferation, migration and invasion of osteosarcoma cells". International Journal of Oncology 52.4 (2018): 1277-1284.
Chicago
Guo, Y., Yin, J., Tang, M., Yu, X."Downregulation of SOX3 leads to the inhibition of the proliferation, migration and invasion of osteosarcoma cells". International Journal of Oncology 52, no. 4 (2018): 1277-1284. https://doi.org/10.3892/ijo.2018.4278
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