miR‑15a represses cancer cell migration and invasion under conditions of hypoxia by targeting and downregulating Bcl‑2 expression in human osteosarcoma cells

Leng,Jiali; Song,Qingxu; Zhao,Yuguang; Wang,Zhenyu

doi:10.3892/ijo.2018.4285

miR‑15a represses cancer cell migration and invasion under conditions of hypoxia by targeting and downregulating Bcl‑2 expression in human osteosarcoma cells

Authors:
- Jiali Leng
- Qingxu Song
- Yuguang Zhao
- Zhenyu Wang
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Affiliations: Nursing Platform of Bone, Joint and Sports Medicine, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Department of Spinal Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Cancer Center, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China
Published online on: February 23, 2018 https://doi.org/10.3892/ijo.2018.4285
Pages: 1095-1104
Copyright: © Leng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Osteosarcoma is a common, high-risk primary bone malignancy that mostly affects the younger population. There has been no marked improvement in the clinical outcomes of osteosarcoma patients to date, and cancer recurrence and metastasis are common in high-grade osteosarcoma. Therefore, identifying new biomarkers and novel therapeutic targets is crucial for improving the prognosis of osteosarcoma patients. In the present study, the MG63 human osteosarcoma cell line was employed to examine the role of microRNA (miR)‑15a in regulating cellular activities under hypoxic conditions. It was demonstrated that hypoxia stimulates migration and invasion in MG63 cells, which was correlated with the downregulation of miR‑15a and upregulation of B-cell lymphoma 2 (Bcl‑2) expression. Introduction of miR‑15a or knockdown of endogenous Bcl‑2 may reduce hypoxia-induced cell invasion and migration through the regulation of matrix metalloproteinases. Analysis of the expression of miR‑15a indicated that hypoxia repressed the transcription of deleted in lymphocytic leukemia 2 (DLEU2), which is the host gene of miR‑15a. These findings indicated that miR‑15a may be a valuable target for the treatment of osteosarcoma, particularly for patients with high-grade cancer or heavy tumor burden.

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