FLVCR1 promotes the proliferation and tumorigenicity of synovial sarcoma through inhibiting apoptosis and autophagy

  • Authors:
    • Changliang Peng
    • Yan Song
    • Wei Chen
    • Xiaoying Wang
    • Xiaoli Liu
    • Fang Wang
    • Dongjin Wu
    • Shengzhong Ma
    • Xiuwen Wang
    • Chunzheng Gao
  • View Affiliations

  • Published online on: March 8, 2018     https://doi.org/10.3892/ijo.2018.4312
  • Pages:1559-1568
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Abstract

Feline leukemia virus subgroup C receptor 1 (FLVCR1) has been reported to have a crucial role in variety of biological processes, including cell proliferation, cell death, apoptosis, oxidative stress response, cellular differentiation and metabolism. However, little is known about its role in synovial sarcoma (SS). In the current study, FLVCR1 expression was analyzed in two SS cell lines (SW982 and HS-SY-II), and in eight SS tissues and paired adjacent non-tumor tissues using reverse transcription-quantitative polymerase chain reaction, western blot analysis and immunohistochemistry. Lentivirus-mediated short hairpin RNAs were used to knock down FLVCR1 expression in SW982 and HS-SY-II cells. The effects of FLVCR1 knockdown on the cell proliferation, clonogenicity, cell cycle and apoptosis in SS cells were evaluated by MTT, colony formation assay, flow cytometry analysis, western blotting and in vivo tumorigenesis in nude mice. In the current study, gene expression of FLVCR1 was upregulated in SS cell lines (SW982 and HS-SY-II) and SS tissues from patients. The protein levels of FLVCR1 in SS tissues were also significantly higher than in adjacent non-tumor tissues. Furthermore, suppressing the expression of FLVCR1 in SS cells using short hairpin RNA effectively attenuated cell proliferation, colony formation and impaired the cell cycle, and also significantly induced apoptosis and autophagy. In accordance with this, an in vivo tumorigenicity assay in mice demonstrated that suppression of FLVCR1 expression inhibited the growth of SS tumors implanted subcutaneously. Collectively, these results demonstrated that FLVCR1 may act as an oncoprotein, and have key roles in promoting proliferation and tumorigenicity of SS, and this may shed new light on finding novel therapeutic strategies against SS.

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May 2018
Volume 52 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

2016 Impact Factor: 3.079
Ranked #33/217 Oncology
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APA
Peng, C., Song, Y., Chen, W., Wang, X., Liu, X., Wang, F. ... Gao, C. (2018). FLVCR1 promotes the proliferation and tumorigenicity of synovial sarcoma through inhibiting apoptosis and autophagy. International Journal of Oncology, 52, 1559-1568. https://doi.org/10.3892/ijo.2018.4312
MLA
Peng, C., Song, Y., Chen, W., Wang, X., Liu, X., Wang, F., Wu, D., Ma, S., Wang, X., Gao, C."FLVCR1 promotes the proliferation and tumorigenicity of synovial sarcoma through inhibiting apoptosis and autophagy". International Journal of Oncology 52.5 (2018): 1559-1568.
Chicago
Peng, C., Song, Y., Chen, W., Wang, X., Liu, X., Wang, F., Wu, D., Ma, S., Wang, X., Gao, C."FLVCR1 promotes the proliferation and tumorigenicity of synovial sarcoma through inhibiting apoptosis and autophagy". International Journal of Oncology 52, no. 5 (2018): 1559-1568. https://doi.org/10.3892/ijo.2018.4312