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miR‑1 reverses multidrug resistance in gastric cancer cells via downregulation of sorcin through promoting the accumulation of intracellular drugs and apoptosis of cells

  • Authors:
    • Lang‑Mei Deng
    • Tan Tan
    • Tian‑Yi Zhang
    • Xue‑Fei Xiao
    • Huan Gu
  • View Affiliations / Copyright

    Affiliations: Critical Care Center, The Third Xiangya Hospital of Central South University, Changsha, Hunan 410013, P.R. China, Department of Inspection, Chenzhou No.1 People's Hospital, Chenzhou, Hunan 423000, P.R. China, Department of Gastroenterology, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China
    Copyright: © Deng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 451-461
    |
    Published online on: June 25, 2019
       https://doi.org/10.3892/ijo.2019.4831
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Abstract

Gastric cancer (GC) is one of the most common cancers worldwide and results in the second greatest rate of cancer‑associated mortality globally. Multidrug resistance (MDR) often develops during the chemotherapy, resulting in the failure of treatment. To investigate the molecular mechanism of MDR, the roles of microRNA (miR)‑1 were studied in GC. Reverse transcription‑quantitative polymerase chain reaction and western blotting were used to investigate the expression levels of miR‑1 and sorcin in SGC7901/ADM and SGC7901/VCR cell lines. The effect of miR‑1 on the half maximal inhibitory concentration (IC50), cell apoptosis rates and drug accumulation was uncovered by MTT assay and flow cytometric analysis. Furthermore, dual‑luciferase assay and western blotting were used to determine the target of miR‑1 in GC. It was demonstrated that miR‑1 was highly downregulated in MDR GC cell lines, including SGC7901/ADM and SGC7901/VCR. Overexpression of miR‑1 in MDR GC cells decreased IC50, but increased the cell apoptosis rates and promoted the drug accumulation in cancer cells. Dual‑luciferase activity assay indicated that sorcin was the target of miR‑1 in GC. In addition, overexpression of sorcin could partially reverse the effect of miR‑1 in MDR GC cells. The role of miR‑1 in MDR GC cells makes it a potential therapeutic target for a successful clinical outcome.
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Copy and paste a formatted citation
Spandidos Publications style
Deng LM, Tan T, Zhang TY, Xiao XF and Gu H: miR‑1 reverses multidrug resistance in gastric cancer cells via downregulation of sorcin through promoting the accumulation of intracellular drugs and apoptosis of cells. Int J Oncol 55: 451-461, 2019.
APA
Deng, L., Tan, T., Zhang, T., Xiao, X., & Gu, H. (2019). miR‑1 reverses multidrug resistance in gastric cancer cells via downregulation of sorcin through promoting the accumulation of intracellular drugs and apoptosis of cells. International Journal of Oncology, 55, 451-461. https://doi.org/10.3892/ijo.2019.4831
MLA
Deng, L., Tan, T., Zhang, T., Xiao, X., Gu, H."miR‑1 reverses multidrug resistance in gastric cancer cells via downregulation of sorcin through promoting the accumulation of intracellular drugs and apoptosis of cells". International Journal of Oncology 55.2 (2019): 451-461.
Chicago
Deng, L., Tan, T., Zhang, T., Xiao, X., Gu, H."miR‑1 reverses multidrug resistance in gastric cancer cells via downregulation of sorcin through promoting the accumulation of intracellular drugs and apoptosis of cells". International Journal of Oncology 55, no. 2 (2019): 451-461. https://doi.org/10.3892/ijo.2019.4831
Copy and paste a formatted citation
x
Spandidos Publications style
Deng LM, Tan T, Zhang TY, Xiao XF and Gu H: miR‑1 reverses multidrug resistance in gastric cancer cells via downregulation of sorcin through promoting the accumulation of intracellular drugs and apoptosis of cells. Int J Oncol 55: 451-461, 2019.
APA
Deng, L., Tan, T., Zhang, T., Xiao, X., & Gu, H. (2019). miR‑1 reverses multidrug resistance in gastric cancer cells via downregulation of sorcin through promoting the accumulation of intracellular drugs and apoptosis of cells. International Journal of Oncology, 55, 451-461. https://doi.org/10.3892/ijo.2019.4831
MLA
Deng, L., Tan, T., Zhang, T., Xiao, X., Gu, H."miR‑1 reverses multidrug resistance in gastric cancer cells via downregulation of sorcin through promoting the accumulation of intracellular drugs and apoptosis of cells". International Journal of Oncology 55.2 (2019): 451-461.
Chicago
Deng, L., Tan, T., Zhang, T., Xiao, X., Gu, H."miR‑1 reverses multidrug resistance in gastric cancer cells via downregulation of sorcin through promoting the accumulation of intracellular drugs and apoptosis of cells". International Journal of Oncology 55, no. 2 (2019): 451-461. https://doi.org/10.3892/ijo.2019.4831
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