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Article Open Access

CXCL1 stimulates migration and invasion in ER‑negative breast cancer cells via activation of the ERK/MMP2/9 signaling axis

  • Authors:
    • Chengcheng Yang
    • Haochen Yu
    • Rui Chen
    • Kai Tao
    • Lei Jian
    • Meixi Peng
    • Xiaotian Li
    • Manran Liu
    • Shengchun Liu
  • View Affiliations / Copyright

    Affiliations: Department of Endocrine and Breast Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China, Department of the Second of Gynecology Oncology, Shanxi Provincial Tumor Hospital, The Affiliated Hospital of Medical College of Xi'an Jiaotong University, Xi'an, Shanxi 710061, P.R. China, Key Laboratory of Laboratory Medical Diagnostics, Chinese Ministry of Education, Chongqing Medical University, Chongqing 400016, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 684-696
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    Published online on: July 15, 2019
       https://doi.org/10.3892/ijo.2019.4840
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Abstract

Chemokine (C‑X‑C motif) ligand 1 (CXCL1), a member of the CXC chemokine family, has been reported to be a critical factor in inflammatory diseases and tumor progression; however, its functions and molecular mechanisms in estrogen receptor α (ER)‑negative breast cancer (BC) remain largely unknown. The present study demonstrated that CXCL1 was upregulated in ER‑negative BC tissues and cell lines compared with ER‑positive tissues and cell lines. Treatment with recombinant human CXCL1 protein promoted ER‑negative BC cell migration and invasion in a dose‑dependent manner, and stimulated the activation of phosphorylated (p)‑ extracellular signal‑regulated kinase (ERK)1/2, but not p‑STAT3 or p‑AKT. Conversely, knockdown of CXCL1 in BC cells attenuated these effects. Additionally, CXCL1 increased the expression of matrix metalloproteinase (MMP)2/9 via the ERK1/2 pathway. Inhibition of MEK1/2 by its antagonist U0126 reversed the effects of CXCL1 on MMP2/9 expression. Furthermore, immunohistochemical analysis revealed a strong positive association between CXCL1 and p‑ERK1/2 expression levels in BC tissues. In conclusion, the present study demonstrated that CXCL1 is highly expressed in ER‑negative BC, and stimulates BC cell migration and invasion via the ERK/MMP2/9 pathway. Therefore, CXCL1 may serve as a potential therapeutic target in ER‑negative BC.
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Copy and paste a formatted citation
Spandidos Publications style
Yang C, Yu H, Chen R, Tao K, Jian L, Peng M, Li X, Liu M and Liu S: CXCL1 stimulates migration and invasion in ER‑negative breast cancer cells via activation of the ERK/MMP2/9 signaling axis. Int J Oncol 55: 684-696, 2019.
APA
Yang, C., Yu, H., Chen, R., Tao, K., Jian, L., Peng, M. ... Liu, S. (2019). CXCL1 stimulates migration and invasion in ER‑negative breast cancer cells via activation of the ERK/MMP2/9 signaling axis. International Journal of Oncology, 55, 684-696. https://doi.org/10.3892/ijo.2019.4840
MLA
Yang, C., Yu, H., Chen, R., Tao, K., Jian, L., Peng, M., Li, X., Liu, M., Liu, S."CXCL1 stimulates migration and invasion in ER‑negative breast cancer cells via activation of the ERK/MMP2/9 signaling axis". International Journal of Oncology 55.3 (2019): 684-696.
Chicago
Yang, C., Yu, H., Chen, R., Tao, K., Jian, L., Peng, M., Li, X., Liu, M., Liu, S."CXCL1 stimulates migration and invasion in ER‑negative breast cancer cells via activation of the ERK/MMP2/9 signaling axis". International Journal of Oncology 55, no. 3 (2019): 684-696. https://doi.org/10.3892/ijo.2019.4840
Copy and paste a formatted citation
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Spandidos Publications style
Yang C, Yu H, Chen R, Tao K, Jian L, Peng M, Li X, Liu M and Liu S: CXCL1 stimulates migration and invasion in ER‑negative breast cancer cells via activation of the ERK/MMP2/9 signaling axis. Int J Oncol 55: 684-696, 2019.
APA
Yang, C., Yu, H., Chen, R., Tao, K., Jian, L., Peng, M. ... Liu, S. (2019). CXCL1 stimulates migration and invasion in ER‑negative breast cancer cells via activation of the ERK/MMP2/9 signaling axis. International Journal of Oncology, 55, 684-696. https://doi.org/10.3892/ijo.2019.4840
MLA
Yang, C., Yu, H., Chen, R., Tao, K., Jian, L., Peng, M., Li, X., Liu, M., Liu, S."CXCL1 stimulates migration and invasion in ER‑negative breast cancer cells via activation of the ERK/MMP2/9 signaling axis". International Journal of Oncology 55.3 (2019): 684-696.
Chicago
Yang, C., Yu, H., Chen, R., Tao, K., Jian, L., Peng, M., Li, X., Liu, M., Liu, S."CXCL1 stimulates migration and invasion in ER‑negative breast cancer cells via activation of the ERK/MMP2/9 signaling axis". International Journal of Oncology 55, no. 3 (2019): 684-696. https://doi.org/10.3892/ijo.2019.4840
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