Characterization of the human zinc finger nfx‑1‑type containing 1 encoding ZNFX1 gene and its response to 12‑O‑tetradecanoyl‑13‑acetate in HL‑60 cells

Hamada,Hiroshi; Yamamura,Mayu; Ohi,Hiroto; Kobayashi,Yota; Niwa,Kuniyoshi; Oyama,Takahiro; Mano,Yasunari; Asai,Masashi; Tanuma,Sei‑Ichi; Uchiumi,Fumiaki

doi:10.3892/ijo.2019.4860

Characterization of the human zinc finger nfx‑1‑type containing 1 encoding ZNFX1 gene and its response to 12‑O‑tetradecanoyl‑13‑acetate in HL‑60 cells

Authors:
- Hiroshi Hamada
- Mayu Yamamura
- Hiroto Ohi
- Yota Kobayashi
- Kuniyoshi Niwa
- Takahiro Oyama
- Yasunari Mano
- Masashi Asai
- Sei‑Ichi Tanuma
- Fumiaki Uchiumi
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Affiliations: Department of Gene Regulation, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda‑shi, Chiba‑ken 278‑8510, Japan, Department of Clinical Drug Informatics, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda‑shi, Chiba‑ken 278‑8510, Japan, Department of Biochemistry, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda‑shi, Chiba‑ken 278‑8510, Japan
Published online on: August 20, 2019 https://doi.org/10.3892/ijo.2019.4860
Pages: 896-904

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Abstract

Human promyelocytic HL‑60 cells can be differentiated into macrophage‑like cells by treatment with 12‑O‑tetra decanoylphorbol‑13‑acetate (TPA). Certain 5' upstream regions of the zinc finger protein (ZNF)‑encoding genes contain duplicated GGAA motifs, which are frequently found in the TPA‑responding gene promoter regions. To examine transcriptional responses to TPA, 5'flanking regions of human zinc finger CCCH‑type containing, antiviral, ZNF252, ZNF343, ZNF555, ZNF782 and zinc finger nfx‑1‑type containing 1 (ZNFX1) genes were isolated by polymerase chain reaction (PCR) and ligated into a multiple‑cloning site of the pGL4.10[luc2] vector. Transient transfection and a luciferase assay revealed that the ZNFX1 promoter most prominently responded to the TPA treatment. Deletion and point mutation experiments indicated that the duplicated GGAA motif in the 100‑bp region positively responded to TPA. In addition, reverse transcription‑quantitative PCR and western blotting showed that the mRNA and protein of ZNFX1 accumulate during the differentiation of HL‑60 cells. These results indicated that expression of the TPA‑inducible ZNFX1 gene, which belongs to the group of interferon‑responsive genes, is regulated by the cis‑action of the duplicated GGAA motif.

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