[Corrigendum] USP22 promotes the G1/S phase transition by upregulating FoxM1 expression via β‑catenin nuclear localization and is associated with poor prognosis in stage II pancreatic ductal adenocarcinoma

• Authors:
  • Zhen Ning
  • Aman Wang
  • Jinxiao Liang
  • Yunpeng Xie
  • Jiwei Liu
  • Lu Feng
  • Qiu Yan
  • Zhongyu Wang

• View Affiliations

  
  Affiliations: Department of General Surgery, The First Affiliated Hospital of Dalian Medical University, Dalian 116011, P.R. China, Department of Oncology, The First Affiliated Hospital of Dalian Medical University, Dalian 116011, P.R. China, Department of Pathology, The First Affiliated Hospital of Dalian Medical University, Dalian 116011, P.R. China, Department of Biochemistry and Molecular Biology, Dalian Medical University, Dalian 116044, P.R. China
• Published online on: September 4, 2019     https://doi.org/10.3892/ijo.2019.4871
• Pages: 1176-1176

• Copyright: © Ning et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Article

ZHEN NING, AMAN WANG, JINXIAO LIANG, YUNPENG XIE, JIWEI LIU, LU FENG, QIU YAN and ZHONGYU WANG

Int J Oncol 45: [Related article:] 1594-1608, 2014; DOI: 10.3892/ijo.2014.2531

Subsequently to the publication of the above paper, the authors have realized that Figs. 3 and 4 contained errors. Two sets of bands in Figs. 3D and 4B were erroneously placed during the process of figure preparation. In Fig. 3D, the β-actin bands for the PANC-1 and CFPAC-1 cells were presented incorrectly, whereas in Fig.4B, the cdk4 bands for the CFPAC-1 cells were incorrect. The corrected versions of Figs. 3 and 4, showing the correct data for the β-actin bands in Fig. 3D and the cdk4 bands for the CFPAC-1 cells in Fig. 4B, are shown opposite.

Figure 3 USP22 and FoxM1 expression in fresh-frozen PDA tissues and cell lines. (A and B) RT-PCR and western blot analysis of USP22 and FoxM1 in the 26 pairs of PDA fresh-frozen tumor tissues and the paired normal carcinoma adjacent tissues. (C and D) RT-PCR and western blot analysis of USP22 and FoxM1 in the poorly differentiated PANC-1 cell line in comparison with the highly differentiated CFPAC-1 cell line.

Figure 4 USP22 regulates the G1/S phase transition and affects the expression of G1 phase-related proteins through FoxM1 in PDA cell lines. (A) Flow cytometry analysis was performed on the PDA cell lines PANC-1 and CFPAC-1 that were transfected by USP22, FoxM1-siRNA or USP22+FoxM1-siRNA. Values are mean ± SD for triplicate samples. (B) Cellular levels of p21, p27, cyclin D1, cdk4, cdk6 in the PDA cell lines PANC-1 and CFPAC-1 that were transfected by USP22, FoxM1-siRNA or USP22+FoxM1-siRNA. (Cand D) CCK-8 analysis was performed on the PDA cell lines PANC-1 and CFPAC-1 which were transfected by USP22, FoxM1-siRNA or USP22+FoxM1-siRNA. Values are mean ± SD for trip-licate samples. *P<0.05, compared with control. **P<0.01, compared with control.

Note that these changes do not affect the interpretation of the data or the conclusions reported in this paper, and all the authors agree to this correction. The authors apologize to the Editor and to the readership of the Journal for any inconvenience caused.

Figure 3 USP22 and FoxM1 expression in fresh-frozen PDA tissues and cell lines. (A and B) RT-PCR and western blot analysis of USP22 and FoxM1 in the 26 pairs of PDA fresh-frozen tumor tissues and the paired normal carcinoma adjacent tissues. (C and D) RT-PCR and western blot analysis of USP22 and FoxM1 in the poorly differentiated PANC-1 cell line in comparison with the highly differentiated CFPAC-1 cell line.

Figure 4 USP22 regulates the G1/S phase transition and affects the expression of G1 phase-related proteins through FoxM1 in PDA cell lines. (A) Flow cytometry analysis was performed on the PDA cell lines PANC-1 and CFPAC-1 that were transfected by USP22, FoxM1-siRNA or USP22+FoxM1-siRNA. Values are mean ± SD for triplicate samples. (B) Cellular levels of p21, p27, cyclin D1, cdk4, cdk6 in the PDA cell lines PANC-1 and CFPAC-1 that were transfected by USP22, FoxM1-siRNA or USP22+FoxM1-siRNA. (Cand D) CCK-8 analysis was performed on the PDA cell lines PANC-1 and CFPAC-1 which were transfected by USP22, FoxM1-siRNA or USP22+FoxM1-siRNA. Values are mean ± SD for trip-licate samples. *P<0.05, compared with control. **P<0.01, compared with control.