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Anisomycin inhibits angiogenesis in ovarian cancer by attenuating the molecular sponge effect of the lncRNA‑Meg3/miR‑421/PDGFRA axis

  • Authors:
    • Weiping Ye
    • Zhentian Ni
    • Shen Yicheng
    • Hao Pan
    • Yongyi Huang
    • Ying Xiong
    • Te Liu
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200086, P.R. China, Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai 200031, P.R. China, Longhua hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200031, P.R. China, College of Pharmacy, Chongqing Medical University, Chongqing, Sichuan 400016, P.R. China, Shanghai Topbiox Co., Ltd., Shanghai 200031, P.R. China, Shanghai Geriatric Institute of Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200031, P.R. China
    Copyright: © Ye et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1296-1312
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    Published online on: September 30, 2019
       https://doi.org/10.3892/ijo.2019.4887
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Abstract

Angiogenesis has an important role in tumour cell growth and metastasis. Anisomycin has been shown to inhibit tumour cell growth. However, whether anisomycin can inhibit angiogenesis of tumours has not been reported. The present study demonstrated that there was a positive correlation between tumour angiogenesis and the number of CD44+/CD133+ serous human ovarian cancer stem cells (HuOCSCs). Subsequently, it was confirmed that anisomycin significantly inhibited the proliferation, invasion, tumorigenic ability and tumour angiogenesis of HuOCSCs. Gene expression profiling by cDNA microarrays revealed that the expression levels of vascular endothelial cell markers, platelet‑derived growth factors, Notch pathway components and 27 tumour angiogenesis‑related genes were significantly decreased in the anisomycin‑treated group compared with the control group. Further experiments demonstrated that the expression levels of endogenous long non‑coding RNA (lncRNA) maternally expressed 3 (Meg3) were significantly decreased in anisomycin‑treated HuOCSCs, whereas the expression levels of microRNA (miR)‑421 were significantly increased. The results of luciferase reporter assays indicated that, when miR‑421 was overexpressed in cells, the luciferase activities of wild‑type platelet derived growth factor receptor α (PDGFRA) 3' untranslated region and Meg3 reporter plasmids were significantly decreased. Overexpression of miR‑421 in HuOCSCs significantly enhanced the anisomycin‑mediated inhibition of HuOCSC proliferation. Taken together, the present results demonstrated that anisomycin inhibited the activation downstream of the Notch1 pathway by attenuating the molecular sponge effect of the lncRNA‑Meg3/miR‑421/PDGFRA axis, ultimately inhibiting angiogenesis, proliferation and invasion in ovarian cancer cells.
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Copy and paste a formatted citation
Spandidos Publications style
Ye W, Ni Z, Yicheng S, Pan H, Huang Y, Xiong Y and Liu T: Anisomycin inhibits angiogenesis in ovarian cancer by attenuating the molecular sponge effect of the lncRNA‑Meg3/miR‑421/PDGFRA axis. Int J Oncol 55: 1296-1312, 2019.
APA
Ye, W., Ni, Z., Yicheng, S., Pan, H., Huang, Y., Xiong, Y., & Liu, T. (2019). Anisomycin inhibits angiogenesis in ovarian cancer by attenuating the molecular sponge effect of the lncRNA‑Meg3/miR‑421/PDGFRA axis. International Journal of Oncology, 55, 1296-1312. https://doi.org/10.3892/ijo.2019.4887
MLA
Ye, W., Ni, Z., Yicheng, S., Pan, H., Huang, Y., Xiong, Y., Liu, T."Anisomycin inhibits angiogenesis in ovarian cancer by attenuating the molecular sponge effect of the lncRNA‑Meg3/miR‑421/PDGFRA axis". International Journal of Oncology 55.6 (2019): 1296-1312.
Chicago
Ye, W., Ni, Z., Yicheng, S., Pan, H., Huang, Y., Xiong, Y., Liu, T."Anisomycin inhibits angiogenesis in ovarian cancer by attenuating the molecular sponge effect of the lncRNA‑Meg3/miR‑421/PDGFRA axis". International Journal of Oncology 55, no. 6 (2019): 1296-1312. https://doi.org/10.3892/ijo.2019.4887
Copy and paste a formatted citation
x
Spandidos Publications style
Ye W, Ni Z, Yicheng S, Pan H, Huang Y, Xiong Y and Liu T: Anisomycin inhibits angiogenesis in ovarian cancer by attenuating the molecular sponge effect of the lncRNA‑Meg3/miR‑421/PDGFRA axis. Int J Oncol 55: 1296-1312, 2019.
APA
Ye, W., Ni, Z., Yicheng, S., Pan, H., Huang, Y., Xiong, Y., & Liu, T. (2019). Anisomycin inhibits angiogenesis in ovarian cancer by attenuating the molecular sponge effect of the lncRNA‑Meg3/miR‑421/PDGFRA axis. International Journal of Oncology, 55, 1296-1312. https://doi.org/10.3892/ijo.2019.4887
MLA
Ye, W., Ni, Z., Yicheng, S., Pan, H., Huang, Y., Xiong, Y., Liu, T."Anisomycin inhibits angiogenesis in ovarian cancer by attenuating the molecular sponge effect of the lncRNA‑Meg3/miR‑421/PDGFRA axis". International Journal of Oncology 55.6 (2019): 1296-1312.
Chicago
Ye, W., Ni, Z., Yicheng, S., Pan, H., Huang, Y., Xiong, Y., Liu, T."Anisomycin inhibits angiogenesis in ovarian cancer by attenuating the molecular sponge effect of the lncRNA‑Meg3/miR‑421/PDGFRA axis". International Journal of Oncology 55, no. 6 (2019): 1296-1312. https://doi.org/10.3892/ijo.2019.4887
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