Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Oncology
Join Editorial Board Propose a Special Issue
Print ISSN: 1019-6439 Online ISSN: 1791-2423
Journal Cover
January-2020 Volume 56 Issue 1

Full Size Image

Cover Legend PDF

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January-2020 Volume 56 Issue 1

Full Size Image

Cover Legend PDF

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Knockdown of Ski decreases osteosarcoma cell proliferation and migration by suppressing the PI3K/Akt signaling pathway

  • Authors:
    • Xin Zhao
    • Yuying Fang
    • Xingwen Wang
    • Zhouyuan Yang
    • Donghai Li
    • Meng Tian
    • Pengde Kang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China, Weifang Maternal and Child Health Hospital, Weifang, Shandong 261000, P.R. China, The Second Clinical Medical College of Lanzhou University, Lanzhou, Gansu 730030, P.R. China, Neurosurgery Research Laboratory, West China Hospital, Sichuan Univerisity, Chengdu, Sichuan 610041, P.R. China
    Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 4.0].
  • Pages: 206-218
    |
    Published online on: November 14, 2019
       https://doi.org/10.3892/ijo.2019.4914
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Ski, an evolutionary conserved protein, is involved in the development of a number of tumors, such as Barrett's esophagus, leukemia, colorectal cancer, gastric cancer, pancreatic cancer, hemangiomas and melanoma. However, studies on the functions of Ski in osteosarcoma (OS) are limited. In this study, firstly the differential expression of Ski in OS tissues and osteochondroma tissues was detected, and the expression of Ski in both human OS cell lines (MG63 and U2OS) and normal osteoblasts (hFoB1.19) was then detected. The results demonstrated that Ski expression was significantly upregulated in both human OS tissues and cell lines. The results led us to hypothesize that Ski may play an essential role in the pathological process of OS. Thus, Ski specific small interfere RNA (Ski‑siRNA) was used. The results revealed that OS cell proliferation was markedly inhibited following the knockdown of Ski, which was identified by CCK8 assay, EdU staining and cell cycle analysis. In addition, OS cell migration was significantly suppressed following Ski knockdown, which was identified by wound healing assay. Moreover, the protein levels of p‑PI3K and p‑Akt in OS cells declined prominently following Ski knockdown. On the whole, the findings of this study revealed that Ski expression was significantly upregulated in OS tissue and OS cells. The knockdown of Ski decreased OS cell proliferation and migration, which was mediated by blocking the PI3K/Akt signaling pathway. Thus, Ski may act as a tumor promoter gene in tumorigenesis, and Ski may prove to be a potential therapeutic target for the treatment of OS.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

Figure 8

View References

1 

Lei Z, Duan H, Zhao T, Zhang Y, Li G, Meng J, Zhang S and Yan W: PARK2 inhibits osteosarcoma cell growth through the JAK2/STAT3/VEGF signaling pathway. Cell Death Dis. 9:3752018. View Article : Google Scholar : PubMed/NCBI

2 

Morrow JJ and Khanna C: Osteosarcoma genetics and epigenetics: Emerging biology and candidate therapies. Crit Rev Oncog. 20:173–197. 2015. View Article : Google Scholar : PubMed/NCBI

3 

Na KY, Bacchini P, Bertoni F, Kim YW and Park YK: Syndecan-4 and fibronectin in osteosarcoma. Pathology. 44:325–330. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Lilic V, Lilic G, Filipovic S, Milosevic J, Tasic M and Stojiljkovic M: Modern treatment of invasive carcinoma of the uterine cervix. J BUON. 14:587–592. 2009.

5 

Carnelio S, Pai K, Rao N, Solomon M and Ahasan A: Metastatic osteosarcoma to the maxilla: A case report and a review of the literature. Quintessence Int. 33:397–399. 2002.PubMed/NCBI

6 

Bonnon C and Atanasoski S: c-Ski in health and disease. Cell Tissue Res. 347:51–64. 2012. View Article : Google Scholar

7 

Li Y, Turck CM, Teumer JK and Stavnezer E: Unique sequence, ski, in sloankettering avian retroviruses with properties of a new cell-derived oncogene. J Virol. 57:1065–1072. 1986.PubMed/NCBI

8 

Luo K, Stroschein SL, Wang W, Chen D, Martens E, Zhou S and Zhou Q: The Ski oncoprotein interacts with the Smad proteins to repress TGFbeta signaling. Genes Dev. 13:2196–2206. 1999. View Article : Google Scholar : PubMed/NCBI

9 

Villanacci V, Bellone G, Battaglia E, Rossi E, Carbone A, Prati A, Verna C, Niola P, Morelli A, Grassini M and Bassotti G: Ski/SnoN expression in the sequence metaplasia-dysplasia-adenocarcinoma of Barrett's esophagus. Hum Pathol. 39:403–409. 2008. View Article : Google Scholar : PubMed/NCBI

10 

Buess M, Terracciano L, Reuter J, Ballabeni P, Boulay JL, Laffer U, Metzger U, Herrmann R and Rochlitz C: Amplification of SKI is a prognostic marker in early colorectal cancer. Neoplasia. 6:207–212. 2004. View Article : Google Scholar : PubMed/NCBI

11 

Nakao T, Kurita N, Komatsu M, Yoshikawa K, Iwata T, Utsunomiya T and Shimada M: Expression of thrombospondin-1 and Ski are prognostic factors in advanced gastric cancer. Int J Clin Oncol. 16:145–152. 2011. View Article : Google Scholar

12 

Heider TR, Lyman S, Schoonhoven R and Behrns KE: Ski promotes tumor growth through abrogation of transforming growth factor-beta signaling in pancreatic cancer. Ann Surg. 246:61–68. 2007. View Article : Google Scholar : PubMed/NCBI

13 

Chen D, Lin Q, Box N, Roop D, Ishii S, Matsuzaki K, Fan T, Hornyak TJ, Reed JA, Stavnezer E, et al: SKI knockdown inhibits human melanoma tumor growth in vivo. Pigment Cell Melanoma Res. 22:761–772. 2009. View Article : Google Scholar : PubMed/NCBI

14 

Fumagalli S, Doneda L, Nomura N and Larizza L: Expression of the c-ski proto-oncogene in human melanoma cell lines. Melanoma Res. 3:23–27. 1993. View Article : Google Scholar : PubMed/NCBI

15 

Zhao X, Zhou KS, Li ZH, Nan W, Wang J, Xia YY and Zhang HH: Knockdown of Ski decreased the reactive astrocytes proliferation in vitro induced by oxygen-glucose deprivation/reoxygenation. J Cell Biochem. 119:4548–4558. 2018. View Article : Google Scholar

16 

Zhao X, Wang XW, Zhou KS, Nan W, Guo YQ, Kou JL, Wang J, Xia YY and Zhang HH: Expression of Ski and its role in astro-cyte proliferation and migration. Neuroscience. 362:1–12. 2017. View Article : Google Scholar : PubMed/NCBI

17 

Wang Z, Sun X, Bao Y, Mo J, Du H, Hu J and Zhang X: E2F1 silencing inhibits migration and invasion of osteosarcoma cells via regulating DDR1 expression. Int J Oncol. 51:1639–1650. 2017. View Article : Google Scholar : PubMed/NCBI

18 

Jiang W, Yu Y, Liu J, Zhao Q, Wang J, Zhang J and Dang X: Downregulation of Cdc6 inhibits tumorigenesis of osteosarcoma in vivo and in vitro. Biomed Pharmacother. 115:1089492019. View Article : Google Scholar : PubMed/NCBI

19 

Chen R, Huang LH, Gao YY, Yang JZ and Wang Y: Identification of differentially expressed genes in MG63 osteosarcoma cells with drug-resistance by microarray analysis. Mol Med Rep. 19:1571–1580. 2019.

20 

Chen R, Wang G, Zheng Y, Hua Y and Cai Z: Drug resistance-related microRNAs in osteosarcoma: Translating basic evidence into therapeutic strategies. J Cell Mol Med. 23:2280–2292. 2019. View Article : Google Scholar : PubMed/NCBI

21 

Kokura K, Kim H, Shinagawa T, Khan MM, Nomura T and Ishii S: The Ski-binding protein C184M negatively regulates tumor growth factor-beta signaling by sequestering the Smad proteins in the cytoplasm. J Biol Chem. 278:20133–20139. 2003. View Article : Google Scholar : PubMed/NCBI

22 

Li J, Li P, Zhang Y, Li GB, Zhou YG, Yang K and Dai SS: c-Ski inhibits the proliferation of vascular smooth muscle cells via suppressing Smad3 signaling but stimulating p38 pathway. Cell Signal. 25:159–167. 2013. View Article : Google Scholar

23 

Li J, Zhao L, He X, Yang T and Yang K: MiR-21 inhibits c-Ski signaling to promote the proliferation of rat vascular smooth muscle cells. Cell Signal. 26:724–729. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Colmenares C and Stavnezer E: The ski oncogene induces muscle differentiation in quail embryo cells. Cell. 59:293–303. 1989. View Article : Google Scholar : PubMed/NCBI

25 

Zhang H and Stavnezer E: Ski regulates muscle terminal differentiation by transcriptional activation of Myog in a complex with Six1 and Eya3. J Biol Chem. 284:2867–2879. 2009. View Article : Google Scholar :

26 

Macias-Silva M, Li W, Leu JI, Crissey MA and Taub R: Up-regulated transcriptional repressors SnoN and Ski bind Smad proteins to antagonize transforming growth factor-beta signals during liver regeneration. J Biol Chem. 277:28483–28490. 2002. View Article : Google Scholar : PubMed/NCBI

27 

Wang L, Hou Y, Sun Y, Zhao L, Tang X, Hu P, Yang J, Zeng Z, Yang G, Cui X and Liu M: c-Ski activates cancer-associated fibroblasts to regulate breast cancer cell invasion. Mol Oncol. 7:1116–1128. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Reed JA, Lin Q, Chen D, Mian IS and Medrano EE: SKI pathways inducing progression of human melanoma. Cancer Metastasis Rev. 24:265–272. 2005. View Article : Google Scholar : PubMed/NCBI

29 

Wang P, Chen Z, Meng ZQ, Fan J, Luo JM, Liang W, Lin JH, Zhou ZH, Chen H, Wang K, et al: Dual role of Ski in pancreatic cancer cells: Tumor-promoting versus metastasis-suppressive function. Carcinogenesis. 30:1497–1506. 2009. View Article : Google Scholar : PubMed/NCBI

30 

Atanasoski S, Notterpek L, Lee HY, Castagner F, Young P, Ehrengruber MU, Meijer D, Sommer L, Stavnezer E, Colmenares C and Suter U: The protooncogene Ski controls schwann cell proliferation and myelination. Neuron. 43:499–511. 2004. View Article : Google Scholar : PubMed/NCBI

31 

Huang J, Dey R, Wang Y, Jakoncic J, Kurinov I and Huang XY: Structural insights into the induced-fit inhibition of fascin by a small-molecule inhibitor. J Mol Biol. 430:1324–1335. 2018. View Article : Google Scholar : PubMed/NCBI

32 

Cao LL, Pei XF, Qiao X, Yu J, Ye H, Xi CL, Wang PY and Gong ZL: SERPINA3 silencing inhibits the migration, invasion, and liver metastasis of colon cancer cells. Dig Dis Sci. 63:2309–2319. 2018. View Article : Google Scholar : PubMed/NCBI

33 

de Oliveira Poswar F, de Carvalho Fraga CA, Gomes ES, Farias LC, Souza LW, Santos SH, Gomez RS, de-Paula AM and Guimarães AL: Protein expression of MMP-2 and MT1-MMP in actinic keratosis, squamous cell carcinoma of the skin, and basal cell carcinoma. Int J Surg Pathol. 23:20–25. 2015. View Article : Google Scholar

34 

Qin H, Liu X, Li F, Miao L, Li T, Xu B, An X, Muth A, Thompson PR, Coonrod SA and Zhang X: PAD1 promotes epithelial-mesenchymal transition and metastasis in triple-negative breast cancer cells by regulating MEK1-ERK1/2-MMP2 signaling. Cancer Lett. 409:30–41. 2017. View Article : Google Scholar : PubMed/NCBI

35 

Li J, Zhu SC, Li SG, Zhao Y, Xu JR and Song CY: TKTL1 promotes cell proliferation and metastasis in esophageal squamous cell carcinoma. Biomed Pharmacother. 74:71–76. 2015. View Article : Google Scholar : PubMed/NCBI

36 

Arndt S, Schmidt J, Wacker E, Karrer S and Bosserhoff AK: Fussel-15, a new player in wound healing, is deregulated in keloid and localized scleroderma. Am J Pathol. 178:2622–2631. 2011. View Article : Google Scholar : PubMed/NCBI

37 

Brader S and Eccles SA: Phosphoinositide 3-kinase signalling pathways in tumor progression, invasion and angiogenesis. Tumori. 90:2–8. 2004. View Article : Google Scholar : PubMed/NCBI

38 

Zhang J, Yu XH, Yan YG, Wang C and Wang WJ: PI3K/Akt signaling in osteosarcoma. Clin Chim Acta. 444:182–192. 2015. View Article : Google Scholar : PubMed/NCBI

39 

Cohen-Solal KA, Boregowda RK and Lasfar A: RUNX2 and the PI3K/AKT axis reciprocal activation as a driving force for tumor progression. Mol Cancer. 14:1372015. View Article : Google Scholar : PubMed/NCBI

40 

Gu QZ, Nijiati A, Gao X, Tao KL, Li CD, Fan XP and Tian Z: TROP2 promotes cell proliferation and migration in osteosarcoma through PI3K/AKT signaling. Mol Med Rep. 18:1782–1788. 2018.PubMed/NCBI

41 

Zhang Y, Cheng H, Li W, Wu H and Yang Y: Highly-expressed P2X7 receptor promotes growth and metastasis of human HOS/MNNG osteosarcoma cells via PI3K/Akt/GSK3β/β-catenin and mTOR/HIF1α/VEGF signaling. Int J Cancer. 145:1068–1082. 2019. View Article : Google Scholar : PubMed/NCBI

42 

Ma J, Huang H, Han Z, Zhu C and Yue B: RLN2 is a positive regulator of Akt-2-induced gene expression required for osteosarcoma cells invasion and chemoresistance. Biomed Res Int. 2015:1474682015. View Article : Google Scholar : PubMed/NCBI

43 

Wang H, Luo QF, Peng AF, Long XH, Wang TF, Liu ZL, Zhang GM, Zhou RP, Gao S, Zhou Y and Chen WZ: Positive feedback regulation between Akt phosphorylation and fatty acid synthase expression in osteosarcoma. Int J Mol Med. 33:633–639. 2014. View Article : Google Scholar

44 

Liu T, Zhou W, Cai B, Chu J, Shi G, Teng H, Xu J, Xiao J and Wang Y: IRX2-mediated upregulation of MMP-9 and VEGF in a PI3K/AKT-dependent manner. Mol Med Rep. 12:4346–4351. 2015. View Article : Google Scholar : PubMed/NCBI

45 

Long XH, Zhong ZH, Peng AF, Zhu LB, Wang H, Zhang GM and Liu ZL: LY294002 suppresses the malignant phenotype and sensitizes osteosarcoma cells to pirarubicin chemotherapy. Mol Med Rep. 10:2967–2972. 2014. View Article : Google Scholar : PubMed/NCBI

46 

Zhou Y, Zhu LB, Peng AF, Wang TF, Long XH, Gao S, Zhou RP and Liu ZL: LY294002 inhibits the malignant phenotype of osteosarcoma cells by modulating the phosphatidylinositol 3-kinase/Akt/fatty acid synthase signaling pathway in vitro. Mol Med Rep. 11:1352–1357. 2015. View Article : Google Scholar

47 

Jiang F, Zhang D, Li G and Wang X: Knockdown of DDX46 inhibits the invasion and tumorigenesis in osteosarcoma cells. Oncol Res. 25:417–425. 2017. View Article : Google Scholar

48 

Chen J, Liu C, Yang QQ, Ma RB, Ke Y, Dong F and Wu XE: Isoliquiritigenin suppresses osteosarcoma U2OS cell proliferation and invasion by regulating the PI3K/Akt signalling pathway. Chemotherapy. 63:155–161. 2018. View Article : Google Scholar : PubMed/NCBI

49 

Band AM, Björklund M and Laiho M: The phosphatidylinositol 3-kinase/Akt pathway regulates transforming growth factor-{beta} signaling by destabilizing ski and inducing Smad7. J Biol Chem. 284:35441–35449. 2009. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Zhao X, Fang Y, Wang X, Yang Z, Li D, Tian M and Kang P: Knockdown of Ski decreases osteosarcoma cell proliferation and migration by suppressing the PI3K/Akt signaling pathway. Int J Oncol 56: 206-218, 2020.
APA
Zhao, X., Fang, Y., Wang, X., Yang, Z., Li, D., Tian, M., & Kang, P. (2020). Knockdown of Ski decreases osteosarcoma cell proliferation and migration by suppressing the PI3K/Akt signaling pathway. International Journal of Oncology, 56, 206-218. https://doi.org/10.3892/ijo.2019.4914
MLA
Zhao, X., Fang, Y., Wang, X., Yang, Z., Li, D., Tian, M., Kang, P."Knockdown of Ski decreases osteosarcoma cell proliferation and migration by suppressing the PI3K/Akt signaling pathway". International Journal of Oncology 56.1 (2020): 206-218.
Chicago
Zhao, X., Fang, Y., Wang, X., Yang, Z., Li, D., Tian, M., Kang, P."Knockdown of Ski decreases osteosarcoma cell proliferation and migration by suppressing the PI3K/Akt signaling pathway". International Journal of Oncology 56, no. 1 (2020): 206-218. https://doi.org/10.3892/ijo.2019.4914
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao X, Fang Y, Wang X, Yang Z, Li D, Tian M and Kang P: Knockdown of Ski decreases osteosarcoma cell proliferation and migration by suppressing the PI3K/Akt signaling pathway. Int J Oncol 56: 206-218, 2020.
APA
Zhao, X., Fang, Y., Wang, X., Yang, Z., Li, D., Tian, M., & Kang, P. (2020). Knockdown of Ski decreases osteosarcoma cell proliferation and migration by suppressing the PI3K/Akt signaling pathway. International Journal of Oncology, 56, 206-218. https://doi.org/10.3892/ijo.2019.4914
MLA
Zhao, X., Fang, Y., Wang, X., Yang, Z., Li, D., Tian, M., Kang, P."Knockdown of Ski decreases osteosarcoma cell proliferation and migration by suppressing the PI3K/Akt signaling pathway". International Journal of Oncology 56.1 (2020): 206-218.
Chicago
Zhao, X., Fang, Y., Wang, X., Yang, Z., Li, D., Tian, M., Kang, P."Knockdown of Ski decreases osteosarcoma cell proliferation and migration by suppressing the PI3K/Akt signaling pathway". International Journal of Oncology 56, no. 1 (2020): 206-218. https://doi.org/10.3892/ijo.2019.4914
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team