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Article

Nimotuzumab, an EGFR‑targeted antibody, promotes radiosensitivity of recurrent esophageal squamous cell carcinoma

  • Authors:
    • Yang Yu
    • Hui Guan
    • Liyang Jiang
    • Xiaolin Li
    • Ligang Xing
    • Xiaorong Sun
  • View Affiliations / Copyright

    Affiliations: School of Medicine and Life Sciences, Shandong Academy of Medical Sciences, University of Jinan, Jinan, Shandong 250031, P.R. China, Department of Radiation Oncology, The Fourth People's Hospital of Jinan, Jinan, Shandong 250031, P.R. China, Department of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong 250117, P.R. China, Department of Nuclear Medicine, Shandong Cancer Hospital Affiliated to Shandong University, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong 250117, P.R. China
  • Pages: 945-956
    |
    Published online on: February 12, 2020
       https://doi.org/10.3892/ijo.2020.4981
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Abstract

Local tumor recurrence is one of the main causes for the failure of esophageal cancer treatment following radiotherapy. Previous studies have demonstrated that epidermal growth factor receptor (EGFR)‑targeted therapy combined with radiotherapy is expected to become an effective means to control tumor recurrence. The aim of the present study was to investigate the effect and mechanism of nimotuzumab (an EGFR‑targeted antibody) in the treatment of recurrent esophageal carcinoma. The radiation responses of two esophageal squamous carcinoma cell lines, EC109 and TE‑1, with or without nimotuzumab, were first evaluated by CCK‑8 assay. Colony formation and apoptosis were used to measure anti‑proliferation effects. It was demonstrated that nimotuzumab arrested the cell cycle at the G2 phase in vitro. Western blotting and immunofluorescence analysis were used to determine signaling pathway changes. It was observed that nimotuzumab inhibited phosphorylation of EGFR in EC109 cells. Furthermore, recurrent tumor models were established and it was identified that the degree of tumor hypoxia was positively associated with EGFR overexpression. In EC109 cell xenografts, nimotuzumab combined with radiation led to a significant delay in recurrent tumor growth compared with that of radiation alone (P<0.001 for 0 Gy pre‑irradiation, P=0.005 for 20 Gy pre‑irradiation, P=0.005 for 10 Gy pre‑irradiation). These results suggest that nimotuzumab combined with radiation may be an effective means to control recurrent esophageal squamous cell carcinoma with EGFR overexpression.
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Copy and paste a formatted citation
Spandidos Publications style
Yu Y, Guan H, Jiang L, Li X, Xing L and Sun X: Nimotuzumab, an EGFR‑targeted antibody, promotes radiosensitivity of recurrent esophageal squamous cell carcinoma. Int J Oncol 56: 945-956, 2020.
APA
Yu, Y., Guan, H., Jiang, L., Li, X., Xing, L., & Sun, X. (2020). Nimotuzumab, an EGFR‑targeted antibody, promotes radiosensitivity of recurrent esophageal squamous cell carcinoma. International Journal of Oncology, 56, 945-956. https://doi.org/10.3892/ijo.2020.4981
MLA
Yu, Y., Guan, H., Jiang, L., Li, X., Xing, L., Sun, X."Nimotuzumab, an EGFR‑targeted antibody, promotes radiosensitivity of recurrent esophageal squamous cell carcinoma". International Journal of Oncology 56.4 (2020): 945-956.
Chicago
Yu, Y., Guan, H., Jiang, L., Li, X., Xing, L., Sun, X."Nimotuzumab, an EGFR‑targeted antibody, promotes radiosensitivity of recurrent esophageal squamous cell carcinoma". International Journal of Oncology 56, no. 4 (2020): 945-956. https://doi.org/10.3892/ijo.2020.4981
Copy and paste a formatted citation
x
Spandidos Publications style
Yu Y, Guan H, Jiang L, Li X, Xing L and Sun X: Nimotuzumab, an EGFR‑targeted antibody, promotes radiosensitivity of recurrent esophageal squamous cell carcinoma. Int J Oncol 56: 945-956, 2020.
APA
Yu, Y., Guan, H., Jiang, L., Li, X., Xing, L., & Sun, X. (2020). Nimotuzumab, an EGFR‑targeted antibody, promotes radiosensitivity of recurrent esophageal squamous cell carcinoma. International Journal of Oncology, 56, 945-956. https://doi.org/10.3892/ijo.2020.4981
MLA
Yu, Y., Guan, H., Jiang, L., Li, X., Xing, L., Sun, X."Nimotuzumab, an EGFR‑targeted antibody, promotes radiosensitivity of recurrent esophageal squamous cell carcinoma". International Journal of Oncology 56.4 (2020): 945-956.
Chicago
Yu, Y., Guan, H., Jiang, L., Li, X., Xing, L., Sun, X."Nimotuzumab, an EGFR‑targeted antibody, promotes radiosensitivity of recurrent esophageal squamous cell carcinoma". International Journal of Oncology 56, no. 4 (2020): 945-956. https://doi.org/10.3892/ijo.2020.4981
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