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E6‑regulated overproduction of prostaglandin E2 may inhibit migration of dendritic cells in human papillomavirus 16‑positive cervical lesions

  • Authors:
    • Jie Huang
    • Ge Diao
    • Qiong Zhang
    • Yajie Chen
    • Jian Han
    • Jianxin Guo
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Daping Hospital, Army Medical University (Third Military Medical University), Chongqing 400042, P.R. China, Institute of Burn Research, Southwest Hospital, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University, Chongqing 400038, P.R. China
    Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 921-931
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    Published online on: February 12, 2020
       https://doi.org/10.3892/ijo.2020.4983
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Abstract

Continuous human papillomavirus (HPV) infection is a critical cause of cervical lesions; however, the specific mechanism is currently not clear. E6 is one of the most important oncoproteins associated with HPV, which regulates synthases in the production of prostaglandin E2 (PGE2). Notably, PGE2 has been reported to be upregulated in cervical lesions. An insufficient number of mature dendritic cells (DCs), which is unable to cause an effective immune response, is an important cause of cervical lesions. Therefore, this study explored the possible causes of HPV16‑positive cervical lesions by identifying the relationship between E6, PGE2 and DCs. Firstly, the distribution and status of DCs in clinical biopsy specimens and animal models were analyzed with immunohistochemistry and flow cytometry, which demonstrated that the migratory ability of DCs was inhibited in HPV16‑positive cervical lesions. Furthermore, using immunohistochemistry, western blotting and ELISA, it was revealed that as the degree of cervical lesions increased, the expression of PGE2 and its synthases increased. Subsequently, as determined using Transwell and 3D migration assays, it was revealed that a high concentration of PGE2 inhibited the migration of DCs, which may explain the phenomenon observed in cervical lesions. Notably, E6 was identified to regulate PGE2 expression. The in vivo experiments indicated that E6 may increase the expression levels of PGE2 in cervical lesions, which could eventually induce inhibition of the migration of DCs. In conclusion, the present study suggested that E6 regulated overproduction of PGE2, which may induce inhibition of DC migration in HPV16‑positive cervical lesions.
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Copy and paste a formatted citation
Spandidos Publications style
Huang J, Diao G, Zhang Q, Chen Y, Han J and Guo J: E6‑regulated overproduction of prostaglandin E2 may inhibit migration of dendritic cells in human papillomavirus 16‑positive cervical lesions. Int J Oncol 56: 921-931, 2020.
APA
Huang, J., Diao, G., Zhang, Q., Chen, Y., Han, J., & Guo, J. (2020). E6‑regulated overproduction of prostaglandin E2 may inhibit migration of dendritic cells in human papillomavirus 16‑positive cervical lesions. International Journal of Oncology, 56, 921-931. https://doi.org/10.3892/ijo.2020.4983
MLA
Huang, J., Diao, G., Zhang, Q., Chen, Y., Han, J., Guo, J."E6‑regulated overproduction of prostaglandin E2 may inhibit migration of dendritic cells in human papillomavirus 16‑positive cervical lesions". International Journal of Oncology 56.4 (2020): 921-931.
Chicago
Huang, J., Diao, G., Zhang, Q., Chen, Y., Han, J., Guo, J."E6‑regulated overproduction of prostaglandin E2 may inhibit migration of dendritic cells in human papillomavirus 16‑positive cervical lesions". International Journal of Oncology 56, no. 4 (2020): 921-931. https://doi.org/10.3892/ijo.2020.4983
Copy and paste a formatted citation
x
Spandidos Publications style
Huang J, Diao G, Zhang Q, Chen Y, Han J and Guo J: E6‑regulated overproduction of prostaglandin E2 may inhibit migration of dendritic cells in human papillomavirus 16‑positive cervical lesions. Int J Oncol 56: 921-931, 2020.
APA
Huang, J., Diao, G., Zhang, Q., Chen, Y., Han, J., & Guo, J. (2020). E6‑regulated overproduction of prostaglandin E2 may inhibit migration of dendritic cells in human papillomavirus 16‑positive cervical lesions. International Journal of Oncology, 56, 921-931. https://doi.org/10.3892/ijo.2020.4983
MLA
Huang, J., Diao, G., Zhang, Q., Chen, Y., Han, J., Guo, J."E6‑regulated overproduction of prostaglandin E2 may inhibit migration of dendritic cells in human papillomavirus 16‑positive cervical lesions". International Journal of Oncology 56.4 (2020): 921-931.
Chicago
Huang, J., Diao, G., Zhang, Q., Chen, Y., Han, J., Guo, J."E6‑regulated overproduction of prostaglandin E2 may inhibit migration of dendritic cells in human papillomavirus 16‑positive cervical lesions". International Journal of Oncology 56, no. 4 (2020): 921-931. https://doi.org/10.3892/ijo.2020.4983
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