Inhibition of basal cell carcinoma cells by cold atmospheric plasma‑activated solution and differential gene expression analysis

Yang,Xingyu; Yang,Chunjun; Wang,Liyun; Cao,Zhicheng; Wang,Yuan; Cheng,Cheng; Zhao,Guoping; Zhao,Ye

doi:10.3892/ijo.2020.5009

Inhibition of basal cell carcinoma cells by cold atmospheric plasma‑activated solution and differential gene expression analysis

Authors:
- Xingyu Yang
- Chunjun Yang
- Liyun Wang
- Zhicheng Cao
- Yuan Wang
- Cheng Cheng
- Guoping Zhao
- Ye Zhao
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Affiliations: Department of Dermatology, The Second Affiliated Hospital, Anhui Medical University, Hefei, Anhui 230601, P.R. China, Department of Orthopaedics, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. China, The Institute of Plasma Physics, Chinese Academy of Sciences, Hefei, Anhui 230031, P.R. China, Anhui Province Key Laboratory of Environmental Toxicology and Pollution Control Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, P.R. China, Teaching and Research Section of Nuclear Medicine, Anhui Medical University, Hefei, Anhui 230032, P.R. China
Published online on: March 6, 2020 https://doi.org/10.3892/ijo.2020.5009
Pages: 1262-1273

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Abstract

Basal cell carcinoma is a common skin tumor. Cold atmospheric plasma (CAP) has attracted increasing attention for its antitumor effects. The aim of the present study was to investigate the effects and related mechanisms of two CAP‑activated solutions on the TE354T basal cell carcinoma and HaCat keratinocyte cell lines. Plasma‑activated solution (PAS) was prepared by CAP irradiation of DMEM and PBS. TE354T cells were treated with PAS in vitro and the effect on cell viability was evaluated by an MTT assay. The apoptosis rate was detected by Annexin V/propidium iodide staining. Furthermore, western blotting and RNA‑sequencing were performed. The present results demonstrated that PAS induced apoptotic signaling in basal cell carcinoma cells, and that this effect was associated with the activation of the MAPK signaling pathway. Therefore, the present study demonstrated that PAS may serve as a novel treatment for basal cell carcinoma.

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