MicroRNA‑500a‑5p inhibits colorectal cancer cell invasion and epithelial‑mesenchymal transition

Tang,Weimei; Hong,Linjie; Dai,Weiyu; Li,Jiaying; Zhu,Huiqiong; Lin,Jianjiao; Yang,Qiong; Wang,Yusi; Lin,Zhizhao; Liu,Mengwei; Xiao,Yizhi; Zhang,Yi; Wu,Xiaosheng; Wang,Jing; Chen,Yaying; Hu,Hongsong; Liu,Side; Wang,Jide; Xiang,Li

doi:10.3892/ijo.2020.5015

MicroRNA‑500a‑5p inhibits colorectal cancer cell invasion and epithelial‑mesenchymal transition

Authors:
- Weimei Tang
- Linjie Hong
- Weiyu Dai
- Jiaying Li
- Huiqiong Zhu
- Jianjiao Lin
- Qiong Yang
- Yusi Wang
- Zhizhao Lin
- Mengwei Liu
- Yizhi Xiao
- Yi Zhang
- Xiaosheng Wu
- Jing Wang
- Yaying Chen
- Hongsong Hu
- Side Liu
- Jide Wang
- Li Xiang
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Affiliations: Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of Gastroenterology, Longgang District People's Hospital, Shenzhen, Guangdong 518172, P.R. China, Department of Gastroenterology, The Second Affiliated Hospital University of South China, Hengyang, Hunan 421001, P.R. China, Department of Gastroenterology, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510150, P.R. China
Published online on: March 19, 2020 https://doi.org/10.3892/ijo.2020.5015
Pages: 1499-1508

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Abstract

The development of malignant tumors is a series of complex processes, the majority of which have not been elucidated. The aim of the present study was to investigate the microRNAs (miRNAs/miR) that affect the migration and invasion abilities of CRC cells. Our previous reports have revealed that miR‑500a‑5p suppressed CRC cell growth and malignant transformation. The present study demonstrated that overexpression of miR‑500a‑5p reduced the expression of vimentin, while increasing the expression of E‑cadherin. Inhibition of miR‑500a‑5p resulted in spindle‑like morphological changes and reorganization of F‑actin in CRC cells. Furthermore, miR‑500a‑5p attenuated the transforming growth factor‑β signaling pathway in EMT. Additionally, emodin inhibited the miR‑500a‑5p inhibitor and suppressed the EMT process. In animal models of metastasis using nude mice, EMT and LoVo cell metastasis was modulated by miR‑500a‑5p. Therefore, the findings of the present study demonstrated that miR‑500a‑5p is associated with a positive therapeutic outcome in terms of invasion/migration of CRC cells and mesenchymal‑like cell changes.

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