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Andrographolide induces apoptosis in human osteosarcoma cells via the ROS/JNK pathway

  • Authors:
    • Shengdong Wang
    • Hengyuan Li
    • Shi Chen
    • Zenan Wang
    • Yuhong Yao
    • Tao Chen
    • Zhaoming Ye
    • Peng Lin
  • View Affiliations / Copyright

    Affiliations: Musculoskeletal Tumor Center, Department of Orthopedics, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang 310009, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1417-1428
    |
    Published online on: March 30, 2020
       https://doi.org/10.3892/ijo.2020.5032
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Abstract

Osteosarcoma is the most common primary malignant tumor of the bone and the long‑term survival of patients with this disease has remained unsatisfactory over the past several decades. Andrographolide, a traditional drug used in Chinese medicine, has been found to exert a significant antitumor effect against several types of cancer. However, relatively little is known about the effect of andrographolide on osteosarcoma and the underlying mechanisms. In the present study, it was shown that andrographolide inhibited osteosarcoma cell proliferation by arresting the cell cycle at the G2/M phase and increasing caspase‑mediated apoptosis. Furthermore, treatment with andrographolide induced JNK activation and increased production of reactive oxygen species (ROS). The andrographolide‑triggered apoptosis in osteosarcoma cells was partly abrogated by a JNK inhibitor and completely reversed by a ROS scavenger. Additionally, JNK activation and cell cycle arrest at the G2/M phase were prevented by administration of an ROS scavenger. In vivo, it was also found that andrographolide inhibited tumor growth by increasing the levels of ROS and activating JNK; thus inducing cytotoxicity in primary osteosarcoma cells. Together, the results of the present study suggest that andrographolide caused G2/M arrest and induced cell apoptosis via regulation of the ROS/JNK signaling pathway in osteosarcoma cells. Thus, andrographolide may serve as a promising antitumor therapeutic agent against osteosarcoma.
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Copy and paste a formatted citation
Spandidos Publications style
Wang S, Li H, Chen S, Wang Z, Yao Y, Chen T, Ye Z and Lin P: Andrographolide induces apoptosis in human osteosarcoma cells via the ROS/JNK pathway. Int J Oncol 56: 1417-1428, 2020.
APA
Wang, S., Li, H., Chen, S., Wang, Z., Yao, Y., Chen, T. ... Lin, P. (2020). Andrographolide induces apoptosis in human osteosarcoma cells via the ROS/JNK pathway. International Journal of Oncology, 56, 1417-1428. https://doi.org/10.3892/ijo.2020.5032
MLA
Wang, S., Li, H., Chen, S., Wang, Z., Yao, Y., Chen, T., Ye, Z., Lin, P."Andrographolide induces apoptosis in human osteosarcoma cells via the ROS/JNK pathway". International Journal of Oncology 56.6 (2020): 1417-1428.
Chicago
Wang, S., Li, H., Chen, S., Wang, Z., Yao, Y., Chen, T., Ye, Z., Lin, P."Andrographolide induces apoptosis in human osteosarcoma cells via the ROS/JNK pathway". International Journal of Oncology 56, no. 6 (2020): 1417-1428. https://doi.org/10.3892/ijo.2020.5032
Copy and paste a formatted citation
x
Spandidos Publications style
Wang S, Li H, Chen S, Wang Z, Yao Y, Chen T, Ye Z and Lin P: Andrographolide induces apoptosis in human osteosarcoma cells via the ROS/JNK pathway. Int J Oncol 56: 1417-1428, 2020.
APA
Wang, S., Li, H., Chen, S., Wang, Z., Yao, Y., Chen, T. ... Lin, P. (2020). Andrographolide induces apoptosis in human osteosarcoma cells via the ROS/JNK pathway. International Journal of Oncology, 56, 1417-1428. https://doi.org/10.3892/ijo.2020.5032
MLA
Wang, S., Li, H., Chen, S., Wang, Z., Yao, Y., Chen, T., Ye, Z., Lin, P."Andrographolide induces apoptosis in human osteosarcoma cells via the ROS/JNK pathway". International Journal of Oncology 56.6 (2020): 1417-1428.
Chicago
Wang, S., Li, H., Chen, S., Wang, Z., Yao, Y., Chen, T., Ye, Z., Lin, P."Andrographolide induces apoptosis in human osteosarcoma cells via the ROS/JNK pathway". International Journal of Oncology 56, no. 6 (2020): 1417-1428. https://doi.org/10.3892/ijo.2020.5032
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