Upregulated expression of SAC3D1 is associated with progression in gastric cancer

Liu,An‑Gui; Zhong,Jin‑Cai; Chen,Gang; He,Rong‑Quan; He,Yi‑Qiang; Ma,Jie; Yang,Li‑Hua; Wu,Xiao‑Jv; Huang,Jun‑Tao; Li,Jian‑Jun; Mo,Wei‑Jia; Qin,Xin‑Gan

doi:10.3892/ijo.2020.5048

Upregulated expression of SAC3D1 is associated with progression in gastric cancer

Authors:
- An‑Gui Liu
- Jin‑Cai Zhong
- Gang Chen
- Rong‑Quan He
- Yi‑Qiang He
- Jie Ma
- Li‑Hua Yang
- Xiao‑Jv Wu
- Jun‑Tao Huang
- Jian‑Jun Li
- Wei‑Jia Mo
- Xin‑Gan Qin
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Affiliations: Department of Oncology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China, Department of Pathology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China, Department of Gastrointestinal Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China, Department of General Surgery, The Second Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530007, P.R. China
Published online on: April 15, 2020 https://doi.org/10.3892/ijo.2020.5048
Pages: 122-138
Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

SAC3 domain containing 1 (SAC3D1) has been reported to be involved in numerous types of cancer. However, the role of SAC3D1 in GC has not yet been elucidated. In the present study, the mRNA expression level of SAC3D1 between GC and normal tissues were assessed with a continuous variable meta‑analysis based on multiple datasets from public databases. The protein expression level of SAC3D1 in GC and normal tissues was assessed by an in‑house immunohistochemistry (IHC). The association between SAC3D1 expression and some clinical parameters was assessed based on the TCGA and IHC data. Survival analysis was performed to assess the association between SAC3D1 expression and the survival of GC patients. The co‑expressed genes of SAC3D1 were determined by integrating three online tools, and the enrichment analyses were performed to determine SAC3D1‑related pathways and hub co‑expressed genes. SAC3D1 was significantly upregulated in GC tumor tissues in comparison to normal tissues with the SMD being 0.45 (0.12, 0.79). The IHC results also indicated that SAC3D1 protein expression in GC tissues was markedly higher than in normal tissues. The SMD following the addition of the IHC data was 0.59 (0.11, 1.07). The protein levels of SAC3D1 were positively associated with the histological grade, T stage and N stage of GC (P<0.001). The TCGA data also revealed that the SAC3D1 mRNA level was significantly associated with the N stage (P<0.001). Moreover, prognosis analysis indicated that SAC3D1 was closely associated with the prognosis of patients with GC. Moreover, 410 co‑expressed genes of SAC3D1 were determined, and these genes were mainly enriched in the cell cycle. In total, 4 genes (CDK1, CCNB1, CCNB2 and CDC20) were considered key co‑expressed genes. On the whole, these findings demonstrate that SAC3D1 is highly expressed in GC and may be associated with the progression of GC.

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