Open Access

circRNA_001275 upregulates Wnt7a expression by competitively sponging miR‑370‑3p to promote cisplatin resistance in esophageal cancer

  • Authors:
    • Fang‑Wen Zou
    • Shi‑Ze Yang
    • Wen‑Ya Li
    • Chao‑Yuan Liu
    • Xu‑Hong Liu
    • Chun‑Hong Hu
    • Zheng‑Hua Liu
    • Shun Xu
  • View Affiliations

  • Published online on: April 22, 2020     https://doi.org/10.3892/ijo.2020.5050
  • Pages: 151-160
  • Copyright: © Zou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Circular RNAs (circRNAs) are aberrantly expressed in various tumors and are associated with tumorigenesis. The present study aimed to determine the role of circRNA_001275 in cisplatin‑resistant esophageal cancer. Three pairs of cisplatin‑resistant tissues and corresponding adjacent tissues were collected and subjected to circRNA chip analysis. Additionally, the effect of circRNA_001275 on cisplatin‑resistant cells was investigated. The relationship between circRNA_001275, microRNAs (miRs) and target genes were analyzed using luciferase assays, and validated via reverse transcription‑quantitative PCR (RT‑qPCR) and western blotting. The results showed that circRNA_001275 was significantly upregulated in cisplatin‑resistant esophageal cancer tissues and cells (P<0.05). Overexpression of circRNA_001275 promoted the proliferation and invasion, and decreased the apoptosis of cisplatin‑resistant cells. On the other hand, circRNA_001275 silencing inhibited cell proliferation and invasion, and promoted cell apoptosis (P<0.05). Dual‑luciferase reporter assays revealed that circRNA_001275 directly binds to miR‑370‑3p, and that Wnt family member 7A (Wnt7a) is targeted by miR‑370‑3p. RT‑qPCR and western blotting further demonstrated that circRNA_001275 serves as an miR‑370‑3p sponge to upregulate Wnt7a expression. In conclusion, the present study revealed that circRNA_001275 was upregulated in cisplatin‑resistant esophageal cancer and promoted cisplatin resistance by sponging miR‑370‑3p to upregulate Wnt7a expression. Therefore, circRNA_001275 may serve as a potential diagnostic biomarker and therapeutic target for patients with cisplatin‑resistant esophageal cancer.

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July 2020
Volume 57 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Zou, F., Yang, S., Li, W., Liu, C., Liu, X., Hu, C. ... Xu, S. (2020). circRNA_001275 upregulates Wnt7a expression by competitively sponging miR‑370‑3p to promote cisplatin resistance in esophageal cancer. International Journal of Oncology, 57, 151-160. https://doi.org/10.3892/ijo.2020.5050
MLA
Zou, F., Yang, S., Li, W., Liu, C., Liu, X., Hu, C., Liu, Z., Xu, S."circRNA_001275 upregulates Wnt7a expression by competitively sponging miR‑370‑3p to promote cisplatin resistance in esophageal cancer". International Journal of Oncology 57.1 (2020): 151-160.
Chicago
Zou, F., Yang, S., Li, W., Liu, C., Liu, X., Hu, C., Liu, Z., Xu, S."circRNA_001275 upregulates Wnt7a expression by competitively sponging miR‑370‑3p to promote cisplatin resistance in esophageal cancer". International Journal of Oncology 57, no. 1 (2020): 151-160. https://doi.org/10.3892/ijo.2020.5050