Open Access

Activating transcription factor 3 inhibits endometrial carcinoma aggressiveness via JunB suppression

  • Authors:
    • Fangyuan Wang
    • Jingjing Li
    • Haixia Wang
    • Fan Zhang
    • Jin Gao
  • View Affiliations

  • Published online on: June 19, 2020     https://doi.org/10.3892/ijo.2020.5084
  • Pages: 707-720
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The function of activating transcription factor 3 (ATF3) in cancer is context‑dependent and its role in endometrial carcinoma (EC) is yet to be elucidated. In the present study, ATF3 was indicated to be downregulated, while one of the ATF3‑interacting proteins, JunB, was upregulated in ECs according to western blot analysis. After overexpression in ECs, ATF3 inhibited the proliferation and invasion of EC cells and enhanced apoptosis, as well as suppressed the expression of JunB. The properties of EC cells, including the expression of matrix metalloproteinases, tissue inhibitors of metalloproteinases, the cell cycle and apoptosis were all altered by overexpression of ATF3. Furthermore, luciferase activity assay, chromatin precipitation and DNA affinity assay results indicated that ATF3 exerted the aforementioned functions via JunB binding and activator protein‑1 signaling. However, the interaction between ATF3 and JunB did not occur in EC cells under basal conditions, but in ATF3‑overexpressing ECs, which was capable of mitigating EC proliferation, invasion and metastasis. Collectively, the present results suggested that the ATF3/JunB interaction may serve as a potential therapeutic target for ECs.
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September-2020
Volume 57 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Copy and paste a formatted citation
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Spandidos Publications style
Wang F, Li J, Wang H, Zhang F and Gao J: Activating transcription factor 3 inhibits endometrial carcinoma aggressiveness via JunB suppression. Int J Oncol 57: 707-720, 2020
APA
Wang, F., Li, J., Wang, H., Zhang, F., & Gao, J. (2020). Activating transcription factor 3 inhibits endometrial carcinoma aggressiveness via JunB suppression. International Journal of Oncology, 57, 707-720. https://doi.org/10.3892/ijo.2020.5084
MLA
Wang, F., Li, J., Wang, H., Zhang, F., Gao, J."Activating transcription factor 3 inhibits endometrial carcinoma aggressiveness via JunB suppression". International Journal of Oncology 57.3 (2020): 707-720.
Chicago
Wang, F., Li, J., Wang, H., Zhang, F., Gao, J."Activating transcription factor 3 inhibits endometrial carcinoma aggressiveness via JunB suppression". International Journal of Oncology 57, no. 3 (2020): 707-720. https://doi.org/10.3892/ijo.2020.5084