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Comparison of the therapeutic effects of lobaplatin and carboplatin on retinoblastoma in vitro and in vivo

  • Authors:
    • Zijun Zhou
    • Hua Jiang
    • Jiejun Xia
    • Jing Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Interventional Radiology and Vascular Anomalies, Guangzhou Women and Children's Medical Center of Guangzhou Medical University, Guangzhou, Guangdong 510627, P.R. China
    Copyright: © Zhou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 697-706
    |
    Published online on: June 22, 2020
       https://doi.org/10.3892/ijo.2020.5085
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Abstract

Retinoblastoma (RB) is one of the most aggressive malignancies affecting infants and children. Platinum drugs are commonly used in the treatment of RB; however, their efficacy is often compromised by drug resistance and severe toxicity. The present study aimed to investigate and compare the toxicity and antitumor activity of the third‑generation platinum drugs, carboplatin and lobaplatin, in vitro and in vivo. The Y79 RB cell line was treated with carboplatin or lobaplatin in vitro and then used to establish xenografts in immunodeficient nude mice in vivo; the effects of pharmacological doses of these drugs were then assessed. High concentrations of carboplatin and lobaplatin markedly inhibited Y79 RB cell proliferation in vitro. In addition, the lobaplatin group exhibited higher proportions of early‑stage apoptotic cells than the carboplatin group, while no significant differences in the proportions of cells in the S phase were observed between the 2 groups, as shown by flow cytometry. Significant changes in the E2F1/Cdc25a/Cdk2 pathway in the RB cells were detected by RNA‑seq following carboplatin or lobaplatin intervention. RT‑qPCR, immunofluorescence and immunohistochemical analyses in vivo and in vitro demonstrated that the trends of drug‑induced inhibition of tumor pathological changes may have been regulated through the E2F1/Cdc25a/Cdk2 pathway, and that lobaplatin was more effective than carboplatin in controlling tumors in vivo. On the whole, the findings of the present study demonstrate that lobaplatin is associated with lower cytotoxicity and exerts more prominent therapeutic effects than carboplatin on Y79 RB cells in vitro and in mice in vivo.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou Z, Jiang H, Xia J and Zhang J: Comparison of the therapeutic effects of lobaplatin and carboplatin on retinoblastoma in vitro and in vivo. Int J Oncol 57: 697-706, 2020.
APA
Zhou, Z., Jiang, H., Xia, J., & Zhang, J. (2020). Comparison of the therapeutic effects of lobaplatin and carboplatin on retinoblastoma in vitro and in vivo. International Journal of Oncology, 57, 697-706. https://doi.org/10.3892/ijo.2020.5085
MLA
Zhou, Z., Jiang, H., Xia, J., Zhang, J."Comparison of the therapeutic effects of lobaplatin and carboplatin on retinoblastoma in vitro and in vivo". International Journal of Oncology 57.3 (2020): 697-706.
Chicago
Zhou, Z., Jiang, H., Xia, J., Zhang, J."Comparison of the therapeutic effects of lobaplatin and carboplatin on retinoblastoma in vitro and in vivo". International Journal of Oncology 57, no. 3 (2020): 697-706. https://doi.org/10.3892/ijo.2020.5085
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou Z, Jiang H, Xia J and Zhang J: Comparison of the therapeutic effects of lobaplatin and carboplatin on retinoblastoma in vitro and in vivo. Int J Oncol 57: 697-706, 2020.
APA
Zhou, Z., Jiang, H., Xia, J., & Zhang, J. (2020). Comparison of the therapeutic effects of lobaplatin and carboplatin on retinoblastoma in vitro and in vivo. International Journal of Oncology, 57, 697-706. https://doi.org/10.3892/ijo.2020.5085
MLA
Zhou, Z., Jiang, H., Xia, J., Zhang, J."Comparison of the therapeutic effects of lobaplatin and carboplatin on retinoblastoma in vitro and in vivo". International Journal of Oncology 57.3 (2020): 697-706.
Chicago
Zhou, Z., Jiang, H., Xia, J., Zhang, J."Comparison of the therapeutic effects of lobaplatin and carboplatin on retinoblastoma in vitro and in vivo". International Journal of Oncology 57, no. 3 (2020): 697-706. https://doi.org/10.3892/ijo.2020.5085
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