SMYD3 overexpression indicates poor prognosis and promotes cell proliferation, migration and invasion in non‑small cell lung cancer
- Authors:
- Published online on: July 7, 2020 https://doi.org/10.3892/ijo.2020.5095
- Pages: 756-766
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Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Abstract
Introduction
Lung cancer is the most commonly diagnosed cancer and the leading cause of cancer-related mortality worldwide, with approximately 2.1 million new cases and 1.8 million deaths in 2018 (1). Non-small cell lung cancer (NSCLC) is the major histological subtype of lung cancer, which accounts for 80-85% of all cases (2). Despite recent advances in the treatment of NSCLC, such as targeted therapy and immunotherapy, the long-term prognosis for NSCLC patients remains poor, and the 5-year survival rate is less than 15% (3). Therefore, it is urgently required to elucidate the detailed molecular pathogenesis of NSCLC, so as to identify more accurate prognostic markers and therapeutic targets.
SET and MYND domain-containing protein 3 (SMYD3) is a member of the SET and MYND domain family, a special class of histone and non-histone lysine methyltransferase (4). SMYD3 functions by catalyzing lysine methylation, mainly through the bi/trimethylation of histone H3 at lysine 4 (H3K4), to render chromatin more accessible and activate downstream gene transcription (5,6). In addition, SMYD3 can transactivate downstream targets by binding to the 5′-CCCTCC-3′ or 5′-GCAGGG-3′ motif in the promoter region of target genes, and interacting with RNA polymerase II and RNA helicase (5). In the past decade, emerging evidence has suggested an oncogenic role of SMYD3 in human cancer. The overexpression of SMYD3 has been observed in breast, hepatocellular, colorectal, gastric, bladder, prostate, cervical, glioma and esophageal squamous cell carcinomas, where it was revealed to be involved in cell proliferation, migration, invasion, epithelial-mesenchymal transition and autophagic activation (7-14). Furthermore, a variable number of tandem repeat (VNTR) polymorphism in the SMYD3 promoter region, namely homozygosity for an allele with three tandem repeats of a CCGCC (3/3), was found to be associated with an increased risk of breast, hepatocellular and colorectal carcinomas (15). As for NSCLC, Barlesi et al found that the SMYD3 VNTR genotype was not correlated with either the tested clinicopathological characteristics or the risk of NSCLC (16). However, the expression of SMYD3 on protein levels in clinical NSCLC samples, as well as the functional role of SMYD3 in NSCLC progression, remain unknown.
In the present study, the expression of SMYD3 in NSCLC and matched adjacent normal tissues was evaluated by immunohistochemistry (IHC), and its clinical and prognostic significance was analyzed. Moreover, the biological roles of SMYD3 in NSCLC cell proliferation, apoptosis, migration, invasion and drug resistance, as well as the molecular basis for such effects, were also characterized. The present findings demonstrated that SMYD3 may serve as an oncogene in NSCLC development and progression, recommending SMYD3 as a promising prognostic marker and therapeutic target for NSCLC.
Materials and methods
Patients and tissue microarray (TMA)
Primary NSCLC and corresponding pericarcinous lung tissues were consecutively collected from patients (89 men, 66 women; mean ± SD age 62.35 ± 10 years, range 38 to 81 years) who underwent surgical resection at Fudan University Shanghai Cancer Center (Shanghai, China) between 2010 and 2012. TMAs were constructed as previously described (17). Two cores with a 1.6-mm diameter were obtained from the original paraffin block of each sample. Clinicopathological information was retrospectively reviewed, and survival data was recorded on the basis of follow-up clinic visits or telephone calls. The pathological stages were determined based on the 7th edition of the lung cancer staging system proposed by the International Association for the Study of Lung Cancer (IASLC) (18). Patients who had received neoadjuvant chemotherapy or radiotherapy, or had a history of other malignancies were excluded. In total, 155 NSCLC cases were enrolled. The present study was approved by the Ethics Committee of Fudan University Shanghai Cancer Center. All experiments were in accordance with the declaration of Helsinki and the approved guidelines of our institution. Written informed consent was obtained from all participants.
IHC
The TMAs were subjected to IHC using anti-SMYD3 antibody (1:800; cat. no. ab187149, Abcam) as previously described (9). Concentration-matched nonspecific rabbit IgG was used as an isotype control. The IHC results were reviewed again by two trained pathologists. Staining intensity was scored as follows: Negative (0), weak (1), moderate (2) and intense (3). The percentage of positive cells was scored as follows: 0% (0), 1-25% (1), 26-50% (2), 51-75% (3) and 76-100% (4). The final SMYD3 IHC score was the multiplication of these two scores, which were defined as low (scores of 0-3) or high (scores of 4-12).
Cell culture and cell proliferation assay
Human NSCLC H727, A549, NCI-H1299, H1650, NCI-H1975, HCC4006, PC-9, HCC827, H2170 and H226 cell lines, and human bronchial epithelial BEAS-2B cell line were obtained from the American Type Culture Collection (ATCC), and cultured in RPMI-1640 or DMEM (Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum (FBS). All cells were authenticated by short tandem repeat profiling (Thermo Fisher Scientific, Inc.) and confirmed to be mycoplasma-free. The in vitro cell proliferation was examined by sulforhodamine B (SRB) assay. Briefly, the cells in 96-well plates were fixed by gently adding cold trichloroacetic acid (100 µl; 10%) and incubated for 30 min at 4°C. The supernatant was discarded, and the plates were washed three times with tap water and air dried. SRB solution (100 µl; 0.4% in 1% acetic acid) was added, and the plates were incubated for 20 min at room temperature. The unconjugated SRB was washed by 1% acetic acid, and the conjugated SRB was dissolved in Tris solution (100 µl; 10 mM). The absorbance was measured at 560 nm using a microplate reader.
siRNA Transfection
Cells (1×105 cells/well in 6-well plates) were transfected with siRNA using Lipofectamine RNAiMAX (Thermo Fisher Scientific, Inc.), following the manufacturer's instructions. The target sequences of double-stranded nucleotides used in siRNA transfection were 5′-GCAGAGTTGTCTTCAAACT-3′(SMYD3siRNA-1) and 5′-GGATGCTGATATGCTAACT-3′(SMYD3siRNA-2) for SMYD3 knockdown, and 5′-TTCTCCGAACGTGTCACGT-3′ as a negative control (NC) (Shanghai GenePharma Co., Ltd.).
Lentiviral vectors and cell infection
Lentiviral vectors were constructed with OBiO Biotechnology, using pRLe nti-CMV-SMYD3-3FLAG-PGK-Puro plasmids, which carry the full-length human SMYD3 (GenBank accession NM_001167740.2) coding sequence. Cell infection was performed as in our previous study (19).
Western blotting
Western blot analysis was performed as previously described (9). The primary antibodies, including anti-SMYD3 (cat. no. ab187149, Abcam), anti-Bcl-2-like protein 11 (Bim; cat. no. 2933S), anti-Bcl-2 homologous antagonist/killer (Bak; cat. no. 6947S), anti-Bax (cat. no. 14796S), anti-B-cell lymphoma 2 (Bcl-2; cat. no. 15071S), anti-Bcl-xl (cat. no. 2764S), anti-matrix metalloproteinase-2 (MMP-2; cat. no. 13132S), anti-MMP-9 (cat. no. 13667S) and anti-β-actin (cat. no. 3700S; all 1:1,000; all from Cell Signaling Technology, Inc.) were used at the manufacturer's recommended dilution. The secondary antibodies were peroxidase AffiniPure goat anti-rabbit IgG (cat. no. 111-035-003) or anti-mouse IgG (cat. no. 115-035-003; both 1:3,000; both from Jackson ImmunoResearch, Inc.). Densitometric analysis was performed using ImageJ software (v1.47, Rawak Software, Inc.). The measured intensities for all samples were first normalized to their respective internal controls and then were compared with BEAS-2B cells, which was set to 100%.
Cell apoptosis analysis
NSCLC cells (1×105 cells per tube) were harvested with EDTA-free trypsin 72 h after transfection, stained using a FITC Annexin V Apoptosis Detection kit (BD Biosciences) following the manufacturer's protocol, and then examined using a flow cytometer (BD Biosciences). For the evaluation of cisplatin resistance, SMYD3-silenced or -over-expressed NSCLC cells were treated with cisplatin (Majorbio Bio-Pharm) at various doses (1, 2 and 4 µM for A549; 0.7, 1.5 and 3 µM for PC-9; 1, 2 and 5 µM for H1299) for 72 h, and then harvested for cell apoptosis analysis.
Cell migration and invasion assays
Cell migration and invasion assays were conducted using the Transwell method, as previously described (12). The number of migrated or invaded cells were counted using a bright-field imaging system on a microscope (Olympus BX51; Olympus Corporation) with five randomly selected fields (magnification, ×200).
Statistical analysis
Statistical analysis was conducted with SPSS v19.0 (IBM Corp.). Comparisons between two groups were performed using Student's t-test. Associations between two categorical variables were examined using Chi-square test or Fisher's exact test. The survival curves were plotted using the Kaplan Meier method, and compared using the log-rank test. Univariate and multivariate Cox proportional hazard models were employed to identify independent predictors. All tests were two sided and P<0.05 was considered to indicate a statistically significant difference.
Results
SMYD3 is upregulated in NSCLC and associated with aggressive clinicopathological characteristics
The expression of SMYD3 was assessed in 155 pairs of NSCLC and corresponding pericarcinous lung tissues by IHC. Representative images revealed that SMYD3 expression was mainly localized in the cytoplasm of NSCLC cells (Fig. 1A), which was consistent with previous studies (20,21). It was determined that SMYD3 was significantly upregulated in NSCLC tissues compared with that in pericarcinous lung tissues (Fig. 1B).
To analyze the association between SMYD3 and clinicopathological characteristics, 155 NSCLC specimens were divided into 'SMYD3 high' (scores of 4-12) and 'SMYD3 low' (scores of 0-3) groups, according to the median expression value. Consistent with the aforementioned findings, the proportion of 'SMYD3 high' samples was significantly higher in NSCLC than in paired normal lung tissues (48.4 vs. 3.2%, P<0.001) (Table I). The present results revealed that a high SMYD3 expression was significantly associated with never-smoked history, more advanced pathological stage, larger tumor size, and the presence of lymphovascular invasion (LVI), pleural invasion (PI) and distant metastasis (Table II).
High SMYD3 expression predicts poor outcomes in NSCLC patients
The prognostic significance of SMYD3 in NSCLC was analyzed. One hundred and thirty-one NSCLC patients qualified for survival analysis, due to sufficient follow-up. It was revealed that disease-free survival (DFS) and overall survival (OS) were significantly worse in patients with a high than in patients with a low SMYD3 expression (Fig. 2A and B).
It was also determined whether SMYD3 has predictive value for clinical outcomes in NSCLC patients. Univariate Cox regression analysis revealed that age at diagnosis (>60 years), advanced pathological stage and PI were correlated with poor DFS in NSCLC patients, and that age at diagnosis (>60 years), advanced pathological stage and high SMYD3 expression were correlated with poor OS (Tables III and IV). Furthermore, multivariate Cox regression analysis demonstrated that advanced pathological stage and PI were independent predictors for poor DFS, and that age at diagnosis (>60 year), advanced pathological stage and high SMYD3 expression were independent predictors for poor OS (Table III and IV).
In combination, the present findings indicated that SMYD3 was upregulated in NSCLC and associated with aggressive clinicopathological characteristics. SMYD3 can serve as an adverse prognostic marker for NSCLC, and may be involved in NSCLC development and progression.
SMYD3 promotes the proliferation of NSCLC cells in vitro
Next, the potential biological role of SMYD3 in NSCLC was investigated. The expression levels of SMYD3 protein in NSCLC cell lines were examined by western blot analysis. As presented in Fig. 3A, SMYD3 protein expression was higher in most NSCLC cell lines (H727, A549, H1975, PC-9, H2170 and H226) compared with that in human bronchial epithelial BEAS-2B cells. H727, A549, H1975, PC-9, H2170 and H226 cells exhibited a higher SMYD3 expression than that of H1299, H1650, HCC4006 or HCC827 cells (Fig. 3A). Next, SMYD3-siRNA transfections were performed in endogenous high-SMYD3-expressing cells. It was determined that SMYD3 knockdown led to a significant reduction in the proliferation rate of A549 and PC-9 cells in vitro (Fig. 3B and C). By contrast, enforced SMYD3 in endogenous low-SMYD3-expressing cells H1299 significantly promoted cell proliferation compared to a mock group (Fig. 3D), indicating an important role of SMYD3 in NSCLC cell proliferation.
SMYD3 knockdown triggers apoptosis by affecting pro-apoptotic and anti-apoptotic proteins in NSCLC cells
Apoptosis is considered an important approach in the inhibition of cell proliferation. In order to elucidate the mechanism underlying SMYD3 knockdown-induced proliferation reduction in NSCLC cells, the apoptotic rate of SMYD3-siRNA-transfected A549 and PC-9 cells was examined. Flow cytometric data revealed that SMYD3 silencing significantly induced apoptosis in both A549 and PC-9 cells (Fig. 4A-C).
To further explore the molecular basis for the induced apoptosis, the expression of pro-apoptotic and anti-apoptotic genes was evaluated. As revealed, the levels of Bim, Bak and Bax were markedly increased in SMYD3-silenced A549 and PC-9 cells, while Bcl-2 and Bcl-xl proteins were decreased upon SMYD3-siRNA transfection in A549 and PC-9 cells (Fig. 4D and E). Collectively, these results indicated that SMYD3 knockdown triggered apoptosis in NSCLC cells, probably by affecting key pro- and anti-apoptotic proteins.
Effect of SMYD3 on cisplatin resistance in NSCLC cells
Since the dysregulated apoptotic signals can result in resistance to anticancer drugs in cancer cells (22), it was speculated that SMYD3 may be involved in cisplatin resistance, a chemotherapy drug for advanced NSCLC (23). As revealed, cisplatin induced apoptosis in A549, PC-9 and H1299 cells in a concentration-dependent manner (Fig. 5A-C). Specifically, SMYD3 silencing sensitized A549 and PC-9 cells to the apoptosis induced by cisplatin (Fig. 5A and B), whereas stable SMYD3 overexpression rendered H1299 cells more resistant to cisplatin-induced apoptosis (Fig. 5C). These data indicated that SMYD3 was associated with resistance to cisplatin in NSCLC cells.
SMYD3 knockdown blocks NSCLC cell migration and invasion in vitro
The effects of SMYD3 on NSCLC cell migration and invasion were determined using Transwell assays. It was determined that SMYD3 knockdown significantly suppressed the migratory and invasive abilities of both A549 and PC-9 cells (Fig. 6A and B). These data indicated the critical role of SMYD3 in the migration and invasion of NSCLC cells.
In addition, the expression of invasion-associated MMP-2 and MMP-9 was assessed in the same transfection experiments. It was observed that the expression levels of both pro- and active-form MMP-2 and MMP-9 were down-regulated upon SMYD3 silencing in A549 and PC-9 cells (Fig. 6C and D).
Discussion
SMYD3 is a lysine methyltransferase that directs the fine regulation of chromatin structure and gene expression (4,24). Recently, it has been revealed that SMYD3 plays significant roles in human carcinogenesis and tumor progression, including in breast, hepatocellular, colorectal and gastric carcinomas (7,8,10). In the present study, it was demonstrated that SMYD3 was upregulated in NSCLC tissues, and associated with aggressive clinicopathological characteristics and poor survival. Multivariate analysis revealed that SMYD3 served as an independent predictor of poor OS in NSCLC patients. It was also revealed that SMYD3 played a critical role in NSCLC cell proliferation, migration, invasion and cisplatin resistance, as well as the regulation of associated genes. The present study is the first, to the best of our knowledge, to comprehensively analyze the expression of SMYD3 in clinical NSCLC samples, and offer insights into the oncogenic potential of SMYD3 in NSCLC carcinogenesis.
Barlesi et al have reported that the SMYD3 VNTR genotype was not correlated with either clinicopathological features or the risk of NSCLC in a Caucasian population (16). However, in the same cohort of NSCLC, there was no correlation between the proposed VNTR genotype and the level of H3K4 dimethylation either, implying that the SMYD3 VNTR genotype may not be equal to the expression or activity of SMYD3 protein in NSCLC. Herein, it was determined that SMYD3 protein was significantly upregulated in NSCLC tissues when compared with paired adjacent normal tissues. Notably, a high SMYD3 expression was associated with aggressive clinicopathological features, such as more advanced pathological stage, larger tumor size and distant metastasis, as well as poor DFS and OS in NSCLC patients. Multivariate Cox regression analysis revealed that SMYD3 overexpression was an independent predictor for poor OS, indicating that SMYD3 may be involved in NSCLC development and progression. In line with our findings, Mazur et al have revealed that abrogating SMYD3 catalytic activity inhibited the formation of Ras-driven lung adenocarcinomas in mouse models (25).
It was previously reported that SMYD3 is essential for the proliferation of cervical, breast, esophageal, hepatocellular and colorectal cancer cells (7,8,14,20). Consistently, the present data revealed that SMYD3 knockdown by siRNA led to a decreased proliferation rate in NSCLC cells in vitro, whereas stable SMYD3 overexpression significantly promoted NSCLC cell proliferation. These data confirmed our hypothesis that SMYD3 behaves as an oncogene in NSCLC carcinogenesis. Given the fact that apoptosis induction is a pivotal mechanism underlying the inhibition of cancer cell proliferation, the apoptosis of SMYD3-silenced NSCLC cells was examined, and it was revealed that SMYD3 silencing significantly induced apoptosis in NSCLC cells. The Bcl-2 protein family members, which can be divided into two classes (pro-apoptotic and anti-apoptotic), have been acknowledged as key regulators of caspase activation and apoptosis (26). Further experiments were then performed to explore the molecular basis for the induced apoptosis in SMYD3-silenced NSCLC cells. It was determined that the levels of pro-apoptotic Bim, Bak and Bax were markedly increased in SMYD3-silenced NSCLC cells, while anti-apoptotic Bcl-2 and Bcl-xl were decreased by SMYD3-siRNA. Our study extends the knowledge derived from Ren et al, who reported that the downregulation of SMYD3 decreased the ratio of Bcl-2/Bax in MDA-MB-231 cells (27), indicating that SMYD3 has a broad effect on apoptosis-associated genes.
Defective or inefficient apoptosis has been considered as a critical mechanism of resistance to anticancer drugs in cancer cells (22). It was then determined whether SMYD3 is involved in resistance to cisplatin, which remains a standard chemotherapy drug for advanced NSCLC (23). The present data revealed that SMYD3 silencing sensitized NSCLC cells to the apoptosis induced by cisplatin, whereas stable SMYD3 overexpression rendered NSCLC cells more resistant to cisplatin-induced apoptosis. This is probably attributed to the change of apoptotic signals in SMYD3-silenced or -over-expressed NSCLC cells. Consistently, previous studies have indicated that cisplatin resistance can be mediated by an inhibition of the apoptotic machinery derived from intracellular events (HER-2/neu amplification, or p53 mutation) in human malignancies (28,29).
In the present study, it was observed that SMYD3 knockdown significantly suppressed NSCLC cell migration and invasion in vitro, highlighting the oncogenic role of SMYD3 in NSCLC progression. Similarly, recent studies revealed that SMYD3 promoted the migration and invasion of multiple cancer types, including hepatocellular, breast and gastric carcinomas (30-32). MMPs are zinc-dependent endopeptidases that participate in tumor progression and metastasis by degrading the extracellular matrix, as well as regulating adherin, cytoskeletal proteins, growth factors, chemokines and cytokines (33). To date, 24 MMPs have been recognized in human beings. In the present study, it was determined that both pro- and active-form MMP-2 and MMP-9 proteins were decreased upon SMYD3 silencing in NSCLC cells, which probably contributed to the impaired invasive activities in those cells. The present results are supported by a previous study, which demonstrated that SMYD3 promoted the trimethylation of H3K4 in the MMP-9 promoter in a reversible model of cancer, thereby inducing MMP-9 expression and increasing cell migration and invasion (6). Based on the present data, it was reasoned that other MMPs, except for MMP-2 and MMP-9, may also be regulated by SMYD3 and involved in SMYD3-mediated cancer migration and invasion, although further studies are required to establish this hypothesis.
The present study has some limitations. First, we were unable to reveal the detailed mechanisms of SMYD3-modulated apoptosis- or invasion-associated genes in NSCLC cells. In addition, another limitation is that although SMYD3 has been recognized to regulate multiple downstream target genes, which are implicated in cell adhesion, proliferation, invasion, apoptosis, cell cycle regulation and signal transduction (5,34,35), only the effects of SMYD3 on NSCLC cell proliferation, apoptosis, migration and invasion were evaluated in the present study. More studies are required to elucidate the effects of other functions of SMYD3 in NSCLC development and progression.
In conclusion, it was demonstrated that SMYD3 was upregulated in NSCLC tissues, and associated with aggressive clinicopathological characteristics and poor survival. SMYD3 overexpression independently predicted poor OS in NSCLC patients. Functionally, SMYD3 played a critical role in NSCLC cell proliferation, migration, invasion and cisplatin resistance, as well as the regulation of apoptosis- and invasion-associated genes. The present study suggested SMYD3 as a novel oncogene in NSCLC carcinogenesis, which is anticipated to be a potential prognostic marker and therapeutic target for NSCLC patients.
Abbreviations:
|
NSCLC |
non-small cell lung cancer |
|
SMYD3 |
SET and MYND domain-containing protein 3 |
|
H3K4 |
histone H3 at lysine 4 |
|
VNTR |
variable number of tandem repeats |
|
IHC |
immunohistochemistry |
|
TMA |
tissue microarray |
|
IASLC |
International Association for the Study of Lung Cancer |
|
ATCC |
American Type Culture Collection |
|
FBS |
fetal bovine serum |
|
SRB |
sulforhodamine B |
|
NC |
negative control |
|
MMP |
matrix metalloproteinase |
|
LVI |
lymphovascular invasion |
|
PI |
pleural invasion |
|
DFS |
disease-free survival |
|
OS |
overall survival |
Acknowledgments
Not applicable.
Funding
The present study was supported in part by grant nos. 81902325 and 81903633 from the National Natural Science Foundation of China, grant no. 2017WS026 from the Health and Family Planning Commission of Shandong Province (China), and grant nos. 2019GSF107042 and 2019GSF107051 from the Key Research and Development Plan of Shandong Province (P.R. China).
Availability of data and materials
All data generated or analyzed in this study are available from the corresponding author on reasonable request.
Authors' contributions
WY devised the conceptual idea. JL, YP and XM acquired and analyzed the clinical data. JL, LZ and LL performed the in vitro experiments. JL, LZ and WW analyzed the data and prepared the figures. JL, LZ and WY wrote, reviewed, and revised the manuscript. All authors read and approved the final manuscript.
Ethics approval and consent to participate
The present study was approved by the Ethics Committee of Fudan University Shanghai Cancer Center. All experiments complied with the Helsinki declaration and the approved guidelines of our institution. Written informed consent was obtained from all participants.
Patient consent for publication
Not applicable.
Competing interests
The authors declare that they have no competing interests.
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