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SMYD3 overexpression indicates poor prognosis and promotes cell proliferation, migration and invasion in non‑small cell lung cancer

  • Authors:
    • Jing Li
    • Lifang Zhao
    • Yunjian Pan
    • Xiao Ma
    • Li Liu
    • Wuzhang Wang
    • Wenjie You
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory and Critical Care Medicine, Shandong Provincial Chest Hospital Affiliated to Shandong University, Jinan, Shandong 250013, P.R. China, Department of Respiratory and Critical Care Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, P.R. China, Department of Thoracic Surgery, Fudan University Shanghai Cancer Center, Shanghai 200032, P.R. China, Department of Respiratory and Critical Care Medicine, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250021, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 756-766
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    Published online on: July 7, 2020
       https://doi.org/10.3892/ijo.2020.5095
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Abstract

SET and MYND domain‑containing protein 3 (SMYD3) is a lysine methyltransferase, and its aberrant expression has been implicated in several malignancies. However, its clinical and biological roles in non‑small cell lung cancer (NSCLC) remain unclear. In the present study, it was revealed that SMYD3 was significantly upregulated in NSCLC tissues, as compared with paired adjacent normal tissues. A high SMYD3 expression was associated with aggressive clinicopathological characteristics, as well as poor disease‑free survival and overall survival (OS) in NSCLC patients. Multivariate analysis revealed that SMYD3 overexpression was an independent predictor of poor OS in NSCLC patients. In addition, SMYD3 knockdown inhibited cell proliferation, triggered apoptosis, and blocked migration and invasion in NSCLC cells in vitro, whereas stable SMYD3 overexpression promoted NSCLC cell proliferation. Furthermore, the SMYD3‑silenced NSCLC cells became more sensitive, whereas the SMYD3‑overexpressed NSCLC cells became more resistant to the apoptosis induced by cisplatin. Mechanistic analysis revealed that SMYD3 knockdown led to the upregulation of Bim, Bak and Bax, and the downregulation of Bcl‑2, Bcl‑xl, MMP‑2 and MMP‑9 in NSCLC cells. In combination, the present findings indicated that SMYD3 has oncogenic potential in the context of NSCLC, providing evidence that may be exploited for both prognostic and therapeutic purposes in the future.
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Copy and paste a formatted citation
Spandidos Publications style
Li J, Zhao L, Pan Y, Ma X, Liu L, Wang W and You W: SMYD3 overexpression indicates poor prognosis and promotes cell proliferation, migration and invasion in non‑small cell lung cancer. Int J Oncol 57: 756-766, 2020.
APA
Li, J., Zhao, L., Pan, Y., Ma, X., Liu, L., Wang, W., & You, W. (2020). SMYD3 overexpression indicates poor prognosis and promotes cell proliferation, migration and invasion in non‑small cell lung cancer. International Journal of Oncology, 57, 756-766. https://doi.org/10.3892/ijo.2020.5095
MLA
Li, J., Zhao, L., Pan, Y., Ma, X., Liu, L., Wang, W., You, W."SMYD3 overexpression indicates poor prognosis and promotes cell proliferation, migration and invasion in non‑small cell lung cancer". International Journal of Oncology 57.3 (2020): 756-766.
Chicago
Li, J., Zhao, L., Pan, Y., Ma, X., Liu, L., Wang, W., You, W."SMYD3 overexpression indicates poor prognosis and promotes cell proliferation, migration and invasion in non‑small cell lung cancer". International Journal of Oncology 57, no. 3 (2020): 756-766. https://doi.org/10.3892/ijo.2020.5095
Copy and paste a formatted citation
x
Spandidos Publications style
Li J, Zhao L, Pan Y, Ma X, Liu L, Wang W and You W: SMYD3 overexpression indicates poor prognosis and promotes cell proliferation, migration and invasion in non‑small cell lung cancer. Int J Oncol 57: 756-766, 2020.
APA
Li, J., Zhao, L., Pan, Y., Ma, X., Liu, L., Wang, W., & You, W. (2020). SMYD3 overexpression indicates poor prognosis and promotes cell proliferation, migration and invasion in non‑small cell lung cancer. International Journal of Oncology, 57, 756-766. https://doi.org/10.3892/ijo.2020.5095
MLA
Li, J., Zhao, L., Pan, Y., Ma, X., Liu, L., Wang, W., You, W."SMYD3 overexpression indicates poor prognosis and promotes cell proliferation, migration and invasion in non‑small cell lung cancer". International Journal of Oncology 57.3 (2020): 756-766.
Chicago
Li, J., Zhao, L., Pan, Y., Ma, X., Liu, L., Wang, W., You, W."SMYD3 overexpression indicates poor prognosis and promotes cell proliferation, migration and invasion in non‑small cell lung cancer". International Journal of Oncology 57, no. 3 (2020): 756-766. https://doi.org/10.3892/ijo.2020.5095
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