Open Access

The double effect of walnut septum extract (Juglans regia L.) counteracts A172 glioblastoma cell survival and bacterial growth

  • Authors:
    • Carlo Genovese
    • Maria Teresa Cambria
    • Floriana D'angeli
    • Alessandro Paolo Addamo
    • Giuseppe Antonio Malfa
    • Laura Siracusa
    • Luana Pulvirenti
    • Carmelina Daniela Anfuso
    • Gabriella Lupo
    • Mario Salmeri
  • View Affiliations

  • Published online on: October 5, 2020     https://doi.org/10.3892/ijo.2020.5130
  • Pages: 1129-1144
  • Copyright : © Genovese et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY 4.0].

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Walnut (Juglans regia L.) is considered to be a ‘superfood’ for its multiple protective actions on human health. Walnut extracts have proven antitumor activity in different cancer cell lines. However, the efficacy of septum extract against glioblastoma has still not been investigated. Glioblastoma is the most difficult type of brain cancer to treat. The standard therapy, based on temozolomide, causes several side effects, including neutropenia and lymphocytopenia, which often favor the onset of opportunistic infections. In the present study, the chemical profile of the Sicilian walnut septum ethanolic extract was analyzed using high‑performance liquid chromatography (HPLC)‑diode array detection and HPLC‑electrospray ionization tandem mass spectrometry. The potential cytostatic activity of the extract against the human A172 glioblastoma cell line was investigated and the results showed that the extract could decrease cancer cell proliferation and migration. Using cytofluorimetric analyses and caspase‑3 assays, the pro‑apoptotic action of walnut extract was demonstrated. Furthermore, the evaluation of the antibacterial activity highlighted the efficacy of the extract in reducing Gram‑positive and Gram‑negative bacterial growth, most of which were resistant to the antibiotic, ciprofloxacin. Finally, Prediction of Activity Spectra for Substances analysis showed the predicted antitumor and antibacterial activity of HPLC detected compounds. The promising results could provide novel perspective in the field of chemotherapeutic co‑adjuvants.
View Figures
View References

Related Articles

Journal Cover

November-2020
Volume 57 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Genovese C, Cambria MT, D'angeli F, Addamo AP, Malfa GA, Siracusa L, Pulvirenti L, Anfuso CD, Lupo G, Salmeri M, Salmeri M, et al: The double effect of walnut septum extract (Juglans regia L.) counteracts A172 glioblastoma cell survival and bacterial growth. Int J Oncol 57: 1129-1144, 2020.
APA
Genovese, C., Cambria, M.T., D'angeli, F., Addamo, A.P., Malfa, G.A., Siracusa, L. ... Salmeri, M. (2020). The double effect of walnut septum extract (Juglans regia L.) counteracts A172 glioblastoma cell survival and bacterial growth. International Journal of Oncology, 57, 1129-1144. https://doi.org/10.3892/ijo.2020.5130
MLA
Genovese, C., Cambria, M. T., D'angeli, F., Addamo, A. P., Malfa, G. A., Siracusa, L., Pulvirenti, L., Anfuso, C. D., Lupo, G., Salmeri, M."The double effect of walnut septum extract (Juglans regia L.) counteracts A172 glioblastoma cell survival and bacterial growth". International Journal of Oncology 57.5 (2020): 1129-1144.
Chicago
Genovese, C., Cambria, M. T., D'angeli, F., Addamo, A. P., Malfa, G. A., Siracusa, L., Pulvirenti, L., Anfuso, C. D., Lupo, G., Salmeri, M."The double effect of walnut septum extract (Juglans regia L.) counteracts A172 glioblastoma cell survival and bacterial growth". International Journal of Oncology 57, no. 5 (2020): 1129-1144. https://doi.org/10.3892/ijo.2020.5130