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Article

Isatin inhibits the invasion and metastasis of SH‑SY5Y neuroblastoma cells in vitro and in vivo

  • Authors:
    • Yanan Hua
    • Na Zhou
    • Jinyu Zhang
    • Zheng Zhang
    • Ning Li
    • Jun Wang
    • Wenxiang Zheng
    • Xue Li
    • Fangling Wang
    • Li Zhang
    • Lin Hou
  • View Affiliations / Copyright

    Affiliations: Department of Neurobiology of Basic Experimental Center Medical College, Medical Department, Qingdao University, Qingdao, Shandong 266021, P.R. China, Department of Biochemistry and Molecular Biology of Basic Experimental Center Medical College, Medical Department, Qingdao University, Qingdao, Shandong 266021, P.R. China, Experimental Center for Undergraduates of Pharmacy, School of Pharmacy, Qingdao University, Qingdao, Shandong 266021, P.R. China
  • Pages: 122-132
    |
    Published online on: November 9, 2020
       https://doi.org/10.3892/ijo.2020.5144
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Abstract

Indoline‑2,3‑dione or indole‑1H‑2,3‑dione, commonly known as isatin, is found in plants of genus Isatin and in Couropita guianancis aubl, and inhibits tumor cell proliferation through its antioxidant effects. The present study analyzed the effect of isatin on the malignant phenotype of neuroblastoma cells, and reported that isatin significantly inhibited neuroblastoma cell proliferation, invasion and migration in vitro in a dose‑dependent manner, and distant metastasis in tumor‑bearing mice. Mechanistically, isatin inhibited lysine‑specific histone demethylase (LSD)1 and reversed the blockade on p53, thereby activating the apoptotic pathway. The inhibitory effect of isatin on LSD1 may be mediated via direct binding and molecular docking or indirectly through the TGFβ/ERK/NF‑κB signaling pathway. Isatin also alleviated the renal and hepatic toxicity of cyclophosphamide in the tumor‑bearing mice, indicating its potential as a candidate drug as well as an adjuvant for treating metastatic neuroblastoma.
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Copy and paste a formatted citation
Spandidos Publications style
Hua Y, Zhou N, Zhang J, Zhang Z, Li N, Wang J, Zheng W, Li X, Wang F, Zhang L, Zhang L, et al: Isatin inhibits the invasion and metastasis of SH‑SY5Y neuroblastoma cells in vitro and in vivo. Int J Oncol 58: 122-132, 2021.
APA
Hua, Y., Zhou, N., Zhang, J., Zhang, Z., Li, N., Wang, J. ... Hou, L. (2021). Isatin inhibits the invasion and metastasis of SH‑SY5Y neuroblastoma cells in vitro and in vivo. International Journal of Oncology, 58, 122-132. https://doi.org/10.3892/ijo.2020.5144
MLA
Hua, Y., Zhou, N., Zhang, J., Zhang, Z., Li, N., Wang, J., Zheng, W., Li, X., Wang, F., Zhang, L., Hou, L."Isatin inhibits the invasion and metastasis of SH‑SY5Y neuroblastoma cells in vitro and in vivo". International Journal of Oncology 58.1 (2021): 122-132.
Chicago
Hua, Y., Zhou, N., Zhang, J., Zhang, Z., Li, N., Wang, J., Zheng, W., Li, X., Wang, F., Zhang, L., Hou, L."Isatin inhibits the invasion and metastasis of SH‑SY5Y neuroblastoma cells in vitro and in vivo". International Journal of Oncology 58, no. 1 (2021): 122-132. https://doi.org/10.3892/ijo.2020.5144
Copy and paste a formatted citation
x
Spandidos Publications style
Hua Y, Zhou N, Zhang J, Zhang Z, Li N, Wang J, Zheng W, Li X, Wang F, Zhang L, Zhang L, et al: Isatin inhibits the invasion and metastasis of SH‑SY5Y neuroblastoma cells in vitro and in vivo. Int J Oncol 58: 122-132, 2021.
APA
Hua, Y., Zhou, N., Zhang, J., Zhang, Z., Li, N., Wang, J. ... Hou, L. (2021). Isatin inhibits the invasion and metastasis of SH‑SY5Y neuroblastoma cells in vitro and in vivo. International Journal of Oncology, 58, 122-132. https://doi.org/10.3892/ijo.2020.5144
MLA
Hua, Y., Zhou, N., Zhang, J., Zhang, Z., Li, N., Wang, J., Zheng, W., Li, X., Wang, F., Zhang, L., Hou, L."Isatin inhibits the invasion and metastasis of SH‑SY5Y neuroblastoma cells in vitro and in vivo". International Journal of Oncology 58.1 (2021): 122-132.
Chicago
Hua, Y., Zhou, N., Zhang, J., Zhang, Z., Li, N., Wang, J., Zheng, W., Li, X., Wang, F., Zhang, L., Hou, L."Isatin inhibits the invasion and metastasis of SH‑SY5Y neuroblastoma cells in vitro and in vivo". International Journal of Oncology 58, no. 1 (2021): 122-132. https://doi.org/10.3892/ijo.2020.5144
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