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Article Open Access

Total flavone extract from Ampelopsis megalophylla induces apoptosis in the MCF‑7 cell line

  • Authors:
    • Chun Gui
    • Chao Zhang
    • Xiaomei Xiong
    • Jing Huang
    • Juan Xi
    • Ling Gong
    • Bisheng Huang
    • Xiuqiao Zhang
  • View Affiliations / Copyright

    Affiliations: The Medicinal Plant Garden, Hubei University of Chinese Medicine, Wuhan, Hubei 430065, P.R. China, Department of Clinical Biochemistry, School of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan, Hubei 430065, P.R. China, Department of Pharmacognosy, School of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan, Hubei 430065, P.R. China
    Copyright: © Gui et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 409-418
    |
    Published online on: January 15, 2021
       https://doi.org/10.3892/ijo.2021.5172
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Abstract

Ampelopsis megalophylla has been found to demonstrate anticancer activities in human cancer cells; however, the effect of total flavone extract (TFE), commonly used in Traditional Chinese Medicine, remains unclear. Furthermore, there is limited information on its effects on breast cancer cell lines. The present study aimed to investigate the inhibitory effects of TFE in different human cancer cell lines. In addition, the underlying mechanisms and the signaling pathways involved were also investigated by determining tumor cell morphological changes, and differences in the cell cycle, apoptosis, mitochondrial transmembrane potential, and related protein expression levels in a breast cancer cell line. It was found that TFE inhibited proliferation in seven cancer cell lines (HeLa, A549, MCF‑7, HepG2, A2780, SW620 and MDA‑MB‑231 and demonstrated a strong inhibitory effect on MCF‑7 cell proliferation. Cell morphological changes were also observed and arrested at the G2/M phase following treatment with TFE at different concentrations. In addition, TFE disrupted the mitochondrial membrane potential and upregulated the expression level of apoptotic proteins, including caspase‑3, ‑8 and ‑9, the Bax/Bcl‑2 ratio, and Apaf‑1 in time‑dependent manner. These results indicated that TFE induced apoptosis of the MCF‑7 cells via a mitochondrial‑mediated apoptotic pathway. In conclusion, TFE is potentially effective in treating breast cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Gui C, Zhang C, Xiong X, Huang J, Xi J, Gong L, Huang B and Zhang X: Total flavone extract from Ampelopsis megalophylla induces apoptosis in the MCF‑7 cell line. Int J Oncol 58: 409-418, 2021.
APA
Gui, C., Zhang, C., Xiong, X., Huang, J., Xi, J., Gong, L. ... Zhang, X. (2021). Total flavone extract from Ampelopsis megalophylla induces apoptosis in the MCF‑7 cell line. International Journal of Oncology, 58, 409-418. https://doi.org/10.3892/ijo.2021.5172
MLA
Gui, C., Zhang, C., Xiong, X., Huang, J., Xi, J., Gong, L., Huang, B., Zhang, X."Total flavone extract from Ampelopsis megalophylla induces apoptosis in the MCF‑7 cell line". International Journal of Oncology 58.3 (2021): 409-418.
Chicago
Gui, C., Zhang, C., Xiong, X., Huang, J., Xi, J., Gong, L., Huang, B., Zhang, X."Total flavone extract from Ampelopsis megalophylla induces apoptosis in the MCF‑7 cell line". International Journal of Oncology 58, no. 3 (2021): 409-418. https://doi.org/10.3892/ijo.2021.5172
Copy and paste a formatted citation
x
Spandidos Publications style
Gui C, Zhang C, Xiong X, Huang J, Xi J, Gong L, Huang B and Zhang X: Total flavone extract from Ampelopsis megalophylla induces apoptosis in the MCF‑7 cell line. Int J Oncol 58: 409-418, 2021.
APA
Gui, C., Zhang, C., Xiong, X., Huang, J., Xi, J., Gong, L. ... Zhang, X. (2021). Total flavone extract from Ampelopsis megalophylla induces apoptosis in the MCF‑7 cell line. International Journal of Oncology, 58, 409-418. https://doi.org/10.3892/ijo.2021.5172
MLA
Gui, C., Zhang, C., Xiong, X., Huang, J., Xi, J., Gong, L., Huang, B., Zhang, X."Total flavone extract from Ampelopsis megalophylla induces apoptosis in the MCF‑7 cell line". International Journal of Oncology 58.3 (2021): 409-418.
Chicago
Gui, C., Zhang, C., Xiong, X., Huang, J., Xi, J., Gong, L., Huang, B., Zhang, X."Total flavone extract from Ampelopsis megalophylla induces apoptosis in the MCF‑7 cell line". International Journal of Oncology 58, no. 3 (2021): 409-418. https://doi.org/10.3892/ijo.2021.5172
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