Open Access

Total flavone extract from Ampelopsis megalophylla induces apoptosis in the MCF‑7 cell line

  • Authors:
    • Chun Gui
    • Chao Zhang
    • Xiaomei Xiong
    • Jing Huang
    • Juan Xi
    • Ling Gong
    • Bisheng Huang
    • Xiuqiao Zhang
  • View Affiliations

  • Published online on: January 15, 2021     https://doi.org/10.3892/ijo.2021.5172
  • Pages: 409-418
  • Copyright: © Gui et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Ampelopsis megalophylla has been found to demonstrate anticancer activities in human cancer cells; however, the effect of total flavone extract (TFE), commonly used in Traditional Chinese Medicine, remains unclear. Furthermore, there is limited information on its effects on breast cancer cell lines. The present study aimed to investigate the inhibitory effects of TFE in different human cancer cell lines. In addition, the underlying mechanisms and the signaling pathways involved were also investigated by determining tumor cell morphological changes, and differences in the cell cycle, apoptosis, mitochondrial transmembrane potential, and related protein expression levels in a breast cancer cell line. It was found that TFE inhibited proliferation in seven cancer cell lines (HeLa, A549, MCF‑7, HepG2, A2780, SW620 and MDA‑MB‑231 and demonstrated a strong inhibitory effect on MCF‑7 cell proliferation. Cell morphological changes were also observed and arrested at the G2/M phase following treatment with TFE at different concentrations. In addition, TFE disrupted the mitochondrial membrane potential and upregulated the expression level of apoptotic proteins, including caspase‑3, ‑8 and ‑9, the Bax/Bcl‑2 ratio, and Apaf‑1 in time‑dependent manner. These results indicated that TFE induced apoptosis of the MCF‑7 cells via a mitochondrial‑mediated apoptotic pathway. In conclusion, TFE is potentially effective in treating breast cancer.
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March-2021
Volume 58 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Gui C, Zhang C, Xiong X, Huang J, Xi J, Gong L, Huang B and Zhang X: Total flavone extract from Ampelopsis megalophylla induces apoptosis in the MCF‑7 cell line. Int J Oncol 58: 409-418, 2021
APA
Gui, C., Zhang, C., Xiong, X., Huang, J., Xi, J., Gong, L. ... Zhang, X. (2021). Total flavone extract from Ampelopsis megalophylla induces apoptosis in the MCF‑7 cell line. International Journal of Oncology, 58, 409-418. https://doi.org/10.3892/ijo.2021.5172
MLA
Gui, C., Zhang, C., Xiong, X., Huang, J., Xi, J., Gong, L., Huang, B., Zhang, X."Total flavone extract from Ampelopsis megalophylla induces apoptosis in the MCF‑7 cell line". International Journal of Oncology 58.3 (2021): 409-418.
Chicago
Gui, C., Zhang, C., Xiong, X., Huang, J., Xi, J., Gong, L., Huang, B., Zhang, X."Total flavone extract from Ampelopsis megalophylla induces apoptosis in the MCF‑7 cell line". International Journal of Oncology 58, no. 3 (2021): 409-418. https://doi.org/10.3892/ijo.2021.5172