APR-246 combined with 3-deazaneplanocin A, panobinostat or temozolomide reduces clonogenicity and induces apoptosis in glioblastoma cells

  • Authors:
    • Javier De La Rosa
    • Alejandro Urdiciain
    • María V. Zelaya
    • Idoya Zazpe
    • Bárbara Meléndez
    • Juan A. Rey
    • Miguel A. Idoate
    • Javier S. Castresana
  • View Affiliations

  • Published online on: January 26, 2021     https://doi.org/10.3892/ijo.2021.5177
  • Pages: 312-330
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Abstract

Glioblastoma is the most malignant brain tumor and presents high resistance to chemotherapy and radiotherapy. Surgery, radiotherapy and chemotherapy with temozolomide are the only treatments against this tumor. New targeted therapies, including epigenetic modulators such as 3‑deazaneplanocin A (DZ‑Nep; an EZH2 inhibitor) and panobinostat (a histone deacetylase inhibitor) are being tested in vitro, together with temozolomide. The present study combined APR‑246 with DZ‑Nep, panobinostat and teomozolomide in order to explore the possibility of restoring p53 function in mutated cases of glioblastoma. Following the Chou‑Talalay method it was demonstrated that APR‑246 acts in an additive manner together with the other compounds, reducing clonogenicity and inducing apoptosis in glioblastoma cells independently of p53 status.
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March-2021
Volume 58 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
De La Rosa J, Urdiciain A, Zelaya MV, Zazpe I, Meléndez B, Rey JA, Idoate MA and Castresana JS: APR-246 combined with 3-deazaneplanocin A, panobinostat or temozolomide reduces clonogenicity and induces apoptosis in glioblastoma cells. Int J Oncol 58: 312-330, 2021
APA
De La Rosa, J., Urdiciain, A., Zelaya, M.V., Zazpe, I., Meléndez, B., Rey, J.A. ... Castresana, J.S. (2021). APR-246 combined with 3-deazaneplanocin A, panobinostat or temozolomide reduces clonogenicity and induces apoptosis in glioblastoma cells. International Journal of Oncology, 58, 312-330. https://doi.org/10.3892/ijo.2021.5177
MLA
De La Rosa, J., Urdiciain, A., Zelaya, M. V., Zazpe, I., Meléndez, B., Rey, J. A., Idoate, M. A., Castresana, J. S."APR-246 combined with 3-deazaneplanocin A, panobinostat or temozolomide reduces clonogenicity and induces apoptosis in glioblastoma cells". International Journal of Oncology 58.3 (2021): 312-330.
Chicago
De La Rosa, J., Urdiciain, A., Zelaya, M. V., Zazpe, I., Meléndez, B., Rey, J. A., Idoate, M. A., Castresana, J. S."APR-246 combined with 3-deazaneplanocin A, panobinostat or temozolomide reduces clonogenicity and induces apoptosis in glioblastoma cells". International Journal of Oncology 58, no. 3 (2021): 312-330. https://doi.org/10.3892/ijo.2021.5177