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Functional assessment of miR‑1291 in colon cancer cells

  • Authors:
    • Jiaqi Wang
    • Yuhki Yokoyama
    • Haruka Hirose
    • Yuki Shimomura
    • Saki Bonkobara
    • Hiroaki Itakura
    • Shihori Kouda
    • Yoshihiro Morimoto
    • Kazumasa Minami
    • Hidekazu Takahashi
    • Satoshi Shibata
    • Shogo Kobayashi
    • Mamoru Uemura
    • Susumu Tanaka
    • Xin Wu
    • Shinji Tanaka
    • Masaki Mori
    • Hirofumi Yamamoto
  • View Affiliations / Copyright

    Affiliations: Department of Molecular Pathology, Division of Health Sciences, Osaka University, Suita, Osaka 565‑0871, Japan, Department of Surgery and Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita, Osaka 565‑0871, Japan, Department of Radiation Oncology, Graduate School of Medicine, Osaka University, Suita, Osaka 565‑0871, Japan, Department of Surgery and Gastroenterological Surgery, Graduate School of Medicine, Osaka University,Suita, Osaka 565‑0871, Japan, First Department of Oral and Maxillofacial Surgery, Graduate School of Dentistry, Osaka University, Suita, Osaka 565‑0871, Japan, Department of Molecular Oncology, Graduate School of Medicine, Tokyo Medical and Dental University, Tokyo 113‑8510, Japan, Tokai University, Graduate School of Medicine, Isehara, Kanagawa 259‑1193, Japan
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 13
    |
    Published online on: January 4, 2022
       https://doi.org/10.3892/ijo.2022.5303
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Abstract

miR‑1291 exerts an anti‑tumor effect in a subset of human carcinomas, including pancreatic cancer. However, its role in colorectal cancer (CRC) is largely unknown. In the present study, the expression and effect of miR‑1291 in CRC cells was investigated. It was identified that miR‑1291 significantly suppressed the proliferation, invasion, cell mobility and colony formation of CRC cells. Additionally, miR‑1291 induced cell apoptosis. A luciferase reporter assay revealed that miR‑1291 directly bound the 3'‑untranslated region sequence of doublecortin‑like kinase 1 (DCLK1). miR‑1291 also suppressed DCLK1 mRNA and protein expression in HCT116 cells that expressed DCLK1. Furthermore, miR‑1291 suppressed cancer stem cell markers BMI1 and CD133, and inhibited sphere formation. The inhibitory effects on sphere formation, invasion and mobility in HCT116 cells were also explored and verified using DCLK1 siRNAs. Furthermore, miR‑1291 induced CDK inhibitors p21WAF1/CIP1 and p27KIP1 in three CRC cell lines, and the overexpression of DCLK1 in HCT116 cells led to a decrease of p21WAF1/CIP1 and p27KIP1. Intravenous administration of miR‑1291 loaded on the super carbonate apatite delivery system significantly inhibited tumor growth in the DLD‑1 xenograft mouse model. Additionally, the resultant tumors exhibited significant upregulation of the p21WAF1/CIP1 and p27KIP1 protein with treatment of miR‑1291. Taken together, the results indicated that miR‑1291 served an anti‑tumor effect by modulating multiple functions, including cancer stemness and cell cycle regulation. The current data suggested that miR‑1291 may be a promising nucleic acid medicine against CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Wang J, Yokoyama Y, Hirose H, Shimomura Y, Bonkobara S, Itakura H, Kouda S, Morimoto Y, Minami K, Takahashi H, Takahashi H, et al: Functional assessment of miR‑1291 in colon cancer cells. Int J Oncol 60: 13, 2022.
APA
Wang, J., Yokoyama, Y., Hirose, H., Shimomura, Y., Bonkobara, S., Itakura, H. ... Yamamoto, H. (2022). Functional assessment of miR‑1291 in colon cancer cells. International Journal of Oncology, 60, 13. https://doi.org/10.3892/ijo.2022.5303
MLA
Wang, J., Yokoyama, Y., Hirose, H., Shimomura, Y., Bonkobara, S., Itakura, H., Kouda, S., Morimoto, Y., Minami, K., Takahashi, H., Shibata, S., Kobayashi, S., Uemura, M., Tanaka, S., Wu, X., Tanaka, S., Mori, M., Yamamoto, H."Functional assessment of miR‑1291 in colon cancer cells". International Journal of Oncology 60.2 (2022): 13.
Chicago
Wang, J., Yokoyama, Y., Hirose, H., Shimomura, Y., Bonkobara, S., Itakura, H., Kouda, S., Morimoto, Y., Minami, K., Takahashi, H., Shibata, S., Kobayashi, S., Uemura, M., Tanaka, S., Wu, X., Tanaka, S., Mori, M., Yamamoto, H."Functional assessment of miR‑1291 in colon cancer cells". International Journal of Oncology 60, no. 2 (2022): 13. https://doi.org/10.3892/ijo.2022.5303
Copy and paste a formatted citation
x
Spandidos Publications style
Wang J, Yokoyama Y, Hirose H, Shimomura Y, Bonkobara S, Itakura H, Kouda S, Morimoto Y, Minami K, Takahashi H, Takahashi H, et al: Functional assessment of miR‑1291 in colon cancer cells. Int J Oncol 60: 13, 2022.
APA
Wang, J., Yokoyama, Y., Hirose, H., Shimomura, Y., Bonkobara, S., Itakura, H. ... Yamamoto, H. (2022). Functional assessment of miR‑1291 in colon cancer cells. International Journal of Oncology, 60, 13. https://doi.org/10.3892/ijo.2022.5303
MLA
Wang, J., Yokoyama, Y., Hirose, H., Shimomura, Y., Bonkobara, S., Itakura, H., Kouda, S., Morimoto, Y., Minami, K., Takahashi, H., Shibata, S., Kobayashi, S., Uemura, M., Tanaka, S., Wu, X., Tanaka, S., Mori, M., Yamamoto, H."Functional assessment of miR‑1291 in colon cancer cells". International Journal of Oncology 60.2 (2022): 13.
Chicago
Wang, J., Yokoyama, Y., Hirose, H., Shimomura, Y., Bonkobara, S., Itakura, H., Kouda, S., Morimoto, Y., Minami, K., Takahashi, H., Shibata, S., Kobayashi, S., Uemura, M., Tanaka, S., Wu, X., Tanaka, S., Mori, M., Yamamoto, H."Functional assessment of miR‑1291 in colon cancer cells". International Journal of Oncology 60, no. 2 (2022): 13. https://doi.org/10.3892/ijo.2022.5303
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