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N6‑methyladenosine upregulates miR‑181d‑5p in exosomes derived from cancer‑associated fibroblasts to inhibit 5‑FU sensitivity by targeting NCALD in colorectal cancer

Retraction in: /10.3892/ijo.2025.5768
  • Authors:
    • Shengli Pan
    • Yingying Deng
    • Jun Fu
    • Yuhao Zhang
    • Zhijin Zhang
    • Xianju Qin
  • View Affiliations / Copyright

    Affiliations: Division of Gastrointestinal Surgery, Department of General Surgery, Shanghai Eighth People Hospital, Jiangsu University, Shanghai 200232, P.R. China, Department of Ophthalmology, Shanghai Eighth People Hospital, Jiangsu University, Shanghai 200232, P.R. China
    Copyright: © Pan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 14
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    Published online on: January 7, 2022
       https://doi.org/10.3892/ijo.2022.5304
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Abstract

Resistance to 5‑Fluorouracil (5‑FU) is a frequent occurrence in patients with colorectal cancer (CRC). MicroRNAs (miRNAs) from cancer‑associated fibroblasts (CAFs)‑secreted exosomes have been associated with 5‑FU sensitivity. The potential molecular mechanism of CAFs‑exosomal miRNAs in CRC remains unclear. The aim of the present study was to elucidate the role of exosomal miRNAs in 5‑FU sensitivity in CRC. Exosomes derived from CAFs were extracted. Exosomal miR‑181d‑5p was identified as a miRNA associated with 5‑FU sensitivity. The putative function of exosomal miR‑181d‑5p was evaluated by ethynyl‑2‑deoxyuridine staining, flow cytometry, RNA immunoprecipitation, luciferase reporter assay, tumor xenograft formation, reverse transcription‑quantitative PCR and western blot analysis. Modification of miR‑181d‑5p by the RNA N6‑methyladenosine (m6A) methyltransferase like (METTL)3 was examined by m6A methylation analysis. The results indicated that m6A modification and METTL3 expression were upregulated in CRC patients. METTL3‑dependent m6A methylation promoted the miR‑181b‑5p process by DiGeorge Syndrome Critical Region 8 (DGCR8) in CAFs. CAFs‑derived exosomes inhibited 5‑FU sensitivity in CRC cells through the METTL3/miR‑181d‑5p axis. A mechanistic study revealed that miR‑181d‑5p directly targeted neurocalcin δ (NCALD) to inhibit the 5‑FU sensitivity of CRC cells. Patients with higher NCALD levels exhibited a higher survival rate. Taken together, METTL3‑dependent m6A methylation was upregulated in CRC to promote the processing of miR‑181d‑5p by DGCR8. This led to increased miR‑181d‑5p expression, which inhibited the 5‑FU sensitivity of CRC cells by targeting NCALD. The results of the present study provided novel insight into exosomal microRNAs in 5‑FU sensitivity in CRC cells. Furthermore, exosomal miR‑181d‑5p may represent a potential prognostic marker for CRC.
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View References

1 

Røed Skårderud M, Polk A, Kjeldgaard Vistisen K, Larsen FO and Nielsen DL: Efficacy and safety of regorafenib in the treatment of metastatic colorectal cancer: A systematic review. Cancer Treat Rev. 62:61–73. 2018. View Article : Google Scholar

2 

Dekker E, Tanis PJ, Vleugels JLA, Kasi PM and Wallace MB: Colorectal cancer. Lancet. 394:1467–1480. 2019. View Article : Google Scholar : PubMed/NCBI

3 

Provenzale D and Gray RN: Colorectal cancer screening and treatment: Review of outcomes research. J Natl Cancer Inst Monogr. 45–55. 2004. View Article : Google Scholar : PubMed/NCBI

4 

Jullumstrø E, Lydersen S, Møller B, Dahl O and Edna TH: Duration of symptoms, stage at diagnosis and relative survival in colon and rectal cancer. Eur J Cancer. 45:2383–2390. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Tsikitis VL, Larson DW, Huebner M, Lohse CM and Thompson PA: Predictors of recurrence free survival for patients with stage II and III colon cancer. BMC Cancer. 14:3362014. View Article : Google Scholar : PubMed/NCBI

6 

Kuebler JP, Wieand HS, O'Connell MJ, Smith RE, Colangelo LH, Yothers G, Petrelli NJ, Findlay MP, Seay TE, Atkins JN, et al: Oxaliplatin combined with weekly bolus fluorouracil and leucovorin as surgical adjuvant chemotherapy for stage II and III colon cancer: Results from NSABP C-07. J Clin Oncol. 25:2198–2204. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Benson AB III, Bekaii-Saab T, Chan E, Chen YJ, Choti MA, Cooper HS, Engstrom PF, Enzinger PC, Fakih MG, Fenton MJ, et al: Metastatic colon cancer, version 3.2013: Featured updates to the NCCN guidelines. J Natl Compr Canc Netw. 11:141–152. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Xu F, Ye ML, Zhang YP, Li WJ, Li MT, Wang HZ, Qiu X, Xu Y, Yin JW, Hu Q, et al: MicroRNA-375-3p enhances chemosensitivity to 5-fluorouracil by targeting thymidylate synthase in colorectal cancer. Cancer Sci. 111:1528–1541. 2020. View Article : Google Scholar : PubMed/NCBI

9 

Liu T, Han C, Wang S, Fang P, Ma Z, Xu L and Yin R: Cancer-associated fibroblasts: An emerging target of anti-cancer immunotherapy. J Hematol Oncol. 12:862019. View Article : Google Scholar : PubMed/NCBI

10 

Kalluri R: The biology and function of fibroblasts in cancer. Nat Rev Cancer. 16:582–598. 2016. View Article : Google Scholar : PubMed/NCBI

11 

Ziani L, Chouaib S and Thiery J: Alteration of the antitumor immune response by cancer-associated fibroblasts. Front Immunol. 9:4142018. View Article : Google Scholar : PubMed/NCBI

12 

Lambrechts D, Wauters E, Boeckx B, Aibar S, Nittner D, Burton O, Bassez A, Decaluwé H, Pircher A, Van den Eynde K, et al: Phenotype molding of stromal cells in the lung tumor microenvironment. Nat Med. 24:1277–1289. 2018. View Article : Google Scholar : PubMed/NCBI

13 

Keller F, Bruch R, Schneider R, Meier-Hubberten J, Hafner M and Rudolf R: A scaffold-free 3-D co-culture mimics the major features of the reverse warburg effect in vitro. Cells. 9:19002020. View Article : Google Scholar :

14 

Hu JL, Wang W, Lan XL, Zeng ZC, Liang YS, Yan YR, Song FY, Wang FF, Zhu XH, Liao WJ, et al: CAFs secreted exosomes promote metastasis and chemotherapy resistance by enhancing cell stemness and epithelial-mesenchymal transition in colorectal cancer. Mol Cancer. 18:912019. View Article : Google Scholar : PubMed/NCBI

15 

Musa M and Ali A: Cancer-associated fibroblasts of colorectal cancer and their markers: Updates, challenges and translational outlook. Future Oncol. 16:2329–2344. 2020. View Article : Google Scholar : PubMed/NCBI

16 

Sun Z, Shi K, Yang S, Liu J, Zhou Q, Wang G, Song J, Li Z, Zhang Z and Yuan W: Effect of exosomal miRNA on cancer biology and clinical applications. Mol Cancer. 17:1472018. View Article : Google Scholar : PubMed/NCBI

17 

Kalluri R and LeBleu VS: The biology, function, and biomedical applications of exosomes. Science. 367:eaau69772020. View Article : Google Scholar :

18 

Mao L, Li X, Gong S, Yuan H, Jiang Y, Huang W, Sun X and Dang X: Serum exosomes contain ECRG4 mRNA that suppresses tumor growth via inhibition of genes involved in inflammation, cell proliferation, and angiogenesis. Cancer Gene Ther. 25:248–259. 2018. View Article : Google Scholar : PubMed/NCBI

19 

Chaput N and Théry C: Exosomes: Immune properties and potential clinical implementations. Semin Immunopathol. 33:419–440. 2011. View Article : Google Scholar

20 

Kahlert C and Kalluri R: Exosomes in tumor microenvironment influence cancer progression and metastasis. J Mol Med (Berl). 91:431–437. 2013. View Article : Google Scholar

21 

Wang H, Wei H, Wang J, Li L, Chen A and Li Z: MicroRNA-181d-5p-containing exosomes derived from CAFs promote EMT by regulating CDX2/HOXA5 in breast cancer. Mol Ther Nucleic Acids. 19:654–667. 2020. View Article : Google Scholar : PubMed/NCBI

22 

Wei H, Wang J, Xu Z, Li W, Wu X, Zhuo C, Lu Y, Long X, Tang Q and Pu J: Hepatoma cell-derived extracellular vesicles promote liver cancer metastasis by inducing the differentiation of bone marrow stem cells through microRNA-181d-5p and the FAK/Src pathway. Front Cell Dev Biol. 9:6070012021. View Article : Google Scholar : PubMed/NCBI

23 

Huang H, Weng H and Chen J: The biogenesis and precise control of RNA m6A methylation. Trends Genet. 36:44–52. 2020. View Article : Google Scholar

24 

Ma S, Chen C, Ji X, Liu J, Zhou Q, Wang G, Yuan W, Kan Q and Sun Z: The interplay between m6A RNA methylation and noncoding RNA in cancer. J Hematol Oncol. 12:1212019. View Article : Google Scholar : PubMed/NCBI

25 

Kobayashi M, Ohsugi M, Sasako T, Awazawa M, Umehara T, Iwane A, Kobayashi N, Okazaki Y, Kubota N, Suzuki R, et al: The RNA methyltransferase complex of WTAP, METTL3, and METTL14 regulates mitotic clonal expansion in adipogenesis. Mol Cell Biol. 38:e00116–18. 2018. View Article : Google Scholar : PubMed/NCBI

26 

Liu X, Liu L, Dong Z, Li J, Yu Y, Chen X, Ren F, Cui G and Sun R: Expression patterns and prognostic value of m6A-related genes in colorectal cancer. Am J Transl Res. 11:3972–3991. 2019.

27 

Zhang J, Bai R, Li M, Ye H, Wu C, Wang C, Li S, Tan L, Mai D, Li G, et al: Excessive miR-25-3p maturation via N6-methyladenosine stimulated by cigarette smoke promotes pancreatic cancer progression. Nat Commun. 10:18582019. View Article : Google Scholar :

28 

Dang TL, Le CT, Le MN, Nguyen TD, Nguyen TL, Bao S, Li S and Nguyen TA: Select amino acids in DGCR8 are essential for the UGU-pri-miRNA interaction and processing. Commun Biol. 3:3442020. View Article : Google Scholar : PubMed/NCBI

29 

Ma JZ, Yang F, Zhou CC, Liu F, Yuan JH, Wang F, Wang TT, Xu QG, Zhou WP and Sun SH: METTL14 suppresses the metastatic potential of hepatocellular carcinoma by modulating N6-methyladenosine-dependent primary MicroRNA processing. Hepatology. 65:529–543. 2017. View Article : Google Scholar

30 

Alarcón CR, Lee H, Goodarzi H, Halberg N and Tavazoie SF: N6-methyladenosine marks primary microRNAs for processing. Nature. 519:482–485. 2015. View Article : Google Scholar : PubMed/NCBI

31 

Yasuda T, Koiwa M, Yonemura A, Akiyama T, Baba H and Ishimoto T: Protocol to establish cancer-associated fibroblasts from surgically resected tissues and generate senescent fibroblasts. STAR Protoc. 2:1005532021. View Article : Google Scholar : PubMed/NCBI

32 

Yu S, Xia S, Yang D, Wang K, Yeh S, Gao Z and Chang C: Androgen receptor in human prostate cancer-associated fibroblasts promotes prostate cancer epithelial cell growth and invasion. Med Oncol. 30:6742013. View Article : Google Scholar : PubMed/NCBI

33 

Kashima H, Wu RC, Wang Y, Sinno AK, Miyamoto T, Shiozawa T, Wang TL, Fader AN and Shih IeM: Laminin C1 expression by uterine carcinoma cells is associated with tumor progression. Gynecol Oncol. 139:338–344. 2015. View Article : Google Scholar : PubMed/NCBI

34 

Xiong S, Zheng Y, Jiang P, Liu R, Liu X and Chu Y: MicroRNA-7 inhibits the growth of human non-small cell lung cancer A549 cells through targeting BCL-2. Int J Biol Sci. 7:805–814. 2011. View Article : Google Scholar : PubMed/NCBI

35 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar

36 

Yan W, Wu X, Zhou W, Fong MY, Cao M, Liu J, Liu X, Chen CH, Fadare O, Pizzo DP, et al: Cancer-cell-secreted exosomal miR-105 promotes tumour growth through the MYC-dependent metabolic reprogramming of stromal cells. Nat Cell Biol. 20:597–609. 2018. View Article : Google Scholar : PubMed/NCBI

37 

Uchihara T, Miyake K, Yonemura A, Komohara Y, Itoyama R, Koiwa M, Yasuda T, Arima K, Harada K, Eto K, et al: Extracellular vesicles from cancer-associated fibroblasts containing annexin A6 induces FAK-YAP activation by stabilizing β1 integrin, enhancing drug resistance. Cancer Res. 80:3222–3235. 2020. View Article : Google Scholar : PubMed/NCBI

38 

Tabet F, Vickers KC, Cuesta Torres LF, Wiese CB, Shoucri BM, Lambert G, Catherinet C, Prado-Lourenco L, Levin MG, Thacker S, et al: HDL-transferred microRNA-223 regulates ICAM-1 expression in endothelial cells. Nat Commun. 5:32922014. View Article : Google Scholar : PubMed/NCBI

39 

Zhang J, Li S, Li L, Li M, Guo C, Yao J and Mi S: Exosome and exosomal microRNA: Trafficking, sorting, and function. Genomics Proteomics Bioinformatics. 13:17–24. 2015. View Article : Google Scholar : PubMed/NCBI

40 

Bhome R, Del Vecchio F, Lee GH, Bullock MD, Primrose JN, Sayan AE and Mirnezami AH: Exosomal microRNAs (exomiRs): Small molecules with a big role in cancer. Cancer Lett. 420:228–235. 2018. View Article : Google Scholar : PubMed/NCBI

41 

Zhang Y, Geng X, Li Q, Xu J, Tan Y, Xiao M, Song J, Liu F, Fang C and Wang H: m6A modification in RNA: Biogenesis, functions and roles in gliomas. J Exp Clin Cancer Res. 39:1922020. View Article : Google Scholar : PubMed/NCBI

42 

Chen XY, Zhang J and Zhu JS: The role of m6A RNA methylation in human cancer. Mol Cancer. 18:1032019. View Article : Google Scholar

43 

Deng X, Su R, Weng H, Huang H, Li Z and Chen J: RNA N6-methyladenosine modification in cancers: Current status and perspectives. Cell Res. 28:507–517. 2018. View Article : Google Scholar : PubMed/NCBI

44 

Shen C, Xuan B, Yan T, Ma Y, Xu P, Tian X, Zhang X, Cao Y, Ma D, Zhu X, et al: m6A-dependent glycolysis enhances colorectal cancer progression. Mol Cancer. 19:722020. View Article : Google Scholar

45 

Liu J, Eckert MA, Harada BT, Liu SM, Lu Z, Yu K, Tienda SM, Chryplewicz A, Zhu AC, Yang Y, et al: m6A mRNA methylation regulates AKT activity to promote the proliferation and tumorigenicity of endometrial cancer. Nat Cell Biol. 20:1074–1083. 2018. View Article : Google Scholar : PubMed/NCBI

46 

Wang X, Feng J, Xue Y, Guan Z, Zhang D, Liu Z, Gong Z, Wang Q, Huang J, Tang C, et al: Structural basis of N(6)-adenosine methylation by the METTL3-METTL14 complex. Nature. 534:575–578. 2016. View Article : Google Scholar : PubMed/NCBI

47 

Li T, Hu PS, Zuo Z, Lin JF, Li X, Wu QN, Chen ZH, Zeng ZL, Wang F, Zheng J, et al: METTL3 facilitates tumor progression via an m6A-IGF2BP2-dependent mechanism in colorectal carcinoma. Mol Cancer. 18:1122019. View Article : Google Scholar

48 

Wang H, Xu B and Shi J: N6-methyladenosine METTL3 promotes the breast cancer progression via targeting Bcl-2. Gene. 722:1440762020. View Article : Google Scholar

49 

O'Brien J, Hayder H, Zayed Y and Peng C: Overview of microRNA biogenesis, mechanisms of actions, and circulation. Front Endocrinol (Lausanne). 9:4022018. View Article : Google Scholar

50 

Peng Y and Croce CM: The role of MicroRNAs in human cancer. Signal Transduct Target Ther. 1:150042016. View Article : Google Scholar : PubMed/NCBI

51 

Volinia S, Calin GA, Liu CG, Ambs S, Cimmino A, Petrocca F, Visone R, Iorio M, Roldo C, Ferracin M, et al: A microRNA expression signature of human solid tumors defines cancer gene targets. Proc Natl Acad Sci USA. 103:2257–2261. 2006. View Article : Google Scholar : PubMed/NCBI

52 

Sun S, Hang T, Zhang B, Zhu L, Wu Y, Lv X, Huang Q and Yao H: miRNA-708 functions as a tumor suppressor in colorectal cancer by targeting ZEB1 through Akt/mTOR signaling pathway. Am J Transl Res. 11:5338–5356. 2019.PubMed/NCBI

53 

Macfarlane LA and Murphy PR: MicroRNA: Biogenesis, function and role in cancer. Curr Genomics. 11:537–561. 2010. View Article : Google Scholar

54 

Denli AM, Tops BB, Plasterk RH, Ketting RF and Hannon GJ: Processing of primary microRNAs by the microprocessor complex. Nature. 432:231–235. 2004. View Article : Google Scholar : PubMed/NCBI

55 

Guo Y, Tian P, Yang C, Liang Z, Li M, Sims M, Lu L, Zhang Z, Li H, Pfeffer LM and Yue J: Silencing the double-stranded RNA binding protein DGCR8 inhibits ovarian cancer cell proliferation, migration, and invasion. Pharm Res. 32:769–778. 2015. View Article : Google Scholar : PubMed/NCBI

56 

Gao LM, Zheng Y, Wang P, Zheng L, Zhang WL, Di Y, Chen LL, Yin XB, Tian Q, Shi SS and Xu SF: Tumor-suppressive effects of microRNA-181d-5p on non-small-cell lung cancer through the CDKN3-mediated Akt signaling pathway in vivo and in vitro. Am J Physiol Lung Cell Mol Physiol. 316:L918–L933. 2019. View Article : Google Scholar : PubMed/NCBI

57 

Huang L, Wen C, Yang X, Lou Q, Wang X, Che J, Chen J, Yang Z, Wu X, Huang M, et al: PEAK1, acting as a tumor promoter in colorectal cancer, is regulated by the EGFR/KRas signaling axis and miR-181d. Cell Death Dis. 9:2712018. View Article : Google Scholar : PubMed/NCBI

58 

Feng LY and Li L: Low expression of NCALD is associated with chemotherapy resistance and poor prognosis in epithelial ovarian cancer. J Ovarian Res. 13:352020. View Article : Google Scholar : PubMed/NCBI

59 

Dong C, Yin F, Zhu D, Cai X, Chen C and Liu X: NCALD affects drug resistance and prognosis by acting as a ceRNA of CX3CL1 in ovarian cancer. J Cell Biochem. 121:4470–4483. 2020. View Article : Google Scholar : PubMed/NCBI

60 

Sahai E, Astsaturov I, Cukierman E, DeNardo DG, Egeblad M, Evans RM, Fearon D, Greten FR, Hingorani SR, Hunter T, et al: A framework for advancing our understanding of cancer-associated fibroblasts. Nat Rev Cancer. 20:174–186. 2020. View Article : Google Scholar : PubMed/NCBI

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Spandidos Publications style
Pan S, Deng Y, Fu J, Zhang Y, Zhang Z and Qin X: N6‑methyladenosine upregulates miR‑181d‑5p in exosomes derived from cancer‑associated fibroblasts to inhibit 5‑FU sensitivity by targeting NCALD in colorectal cancer Retraction in /10.3892/ijo.2025.5768. Int J Oncol 60: 14, 2022.
APA
Pan, S., Deng, Y., Fu, J., Zhang, Y., Zhang, Z., & Qin, X. (2022). N6‑methyladenosine upregulates miR‑181d‑5p in exosomes derived from cancer‑associated fibroblasts to inhibit 5‑FU sensitivity by targeting NCALD in colorectal cancer Retraction in /10.3892/ijo.2025.5768. International Journal of Oncology, 60, 14. https://doi.org/10.3892/ijo.2022.5304
MLA
Pan, S., Deng, Y., Fu, J., Zhang, Y., Zhang, Z., Qin, X."N6‑methyladenosine upregulates miR‑181d‑5p in exosomes derived from cancer‑associated fibroblasts to inhibit 5‑FU sensitivity by targeting NCALD in colorectal cancer Retraction in /10.3892/ijo.2025.5768". International Journal of Oncology 60.2 (2022): 14.
Chicago
Pan, S., Deng, Y., Fu, J., Zhang, Y., Zhang, Z., Qin, X."N6‑methyladenosine upregulates miR‑181d‑5p in exosomes derived from cancer‑associated fibroblasts to inhibit 5‑FU sensitivity by targeting NCALD in colorectal cancer Retraction in /10.3892/ijo.2025.5768". International Journal of Oncology 60, no. 2 (2022): 14. https://doi.org/10.3892/ijo.2022.5304
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Spandidos Publications style
Pan S, Deng Y, Fu J, Zhang Y, Zhang Z and Qin X: N6‑methyladenosine upregulates miR‑181d‑5p in exosomes derived from cancer‑associated fibroblasts to inhibit 5‑FU sensitivity by targeting NCALD in colorectal cancer Retraction in /10.3892/ijo.2025.5768. Int J Oncol 60: 14, 2022.
APA
Pan, S., Deng, Y., Fu, J., Zhang, Y., Zhang, Z., & Qin, X. (2022). N6‑methyladenosine upregulates miR‑181d‑5p in exosomes derived from cancer‑associated fibroblasts to inhibit 5‑FU sensitivity by targeting NCALD in colorectal cancer Retraction in /10.3892/ijo.2025.5768. International Journal of Oncology, 60, 14. https://doi.org/10.3892/ijo.2022.5304
MLA
Pan, S., Deng, Y., Fu, J., Zhang, Y., Zhang, Z., Qin, X."N6‑methyladenosine upregulates miR‑181d‑5p in exosomes derived from cancer‑associated fibroblasts to inhibit 5‑FU sensitivity by targeting NCALD in colorectal cancer Retraction in /10.3892/ijo.2025.5768". International Journal of Oncology 60.2 (2022): 14.
Chicago
Pan, S., Deng, Y., Fu, J., Zhang, Y., Zhang, Z., Qin, X."N6‑methyladenosine upregulates miR‑181d‑5p in exosomes derived from cancer‑associated fibroblasts to inhibit 5‑FU sensitivity by targeting NCALD in colorectal cancer Retraction in /10.3892/ijo.2025.5768". International Journal of Oncology 60, no. 2 (2022): 14. https://doi.org/10.3892/ijo.2022.5304
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