Open Access

circ‑LRP6 contributes to osteosarcoma progression by regulating the miR‑141‑3p/HDAC4/HMGB1 axis

  • Authors:
    • Yali Yu
    • Guixiang Dong
    • Zijun Li
    • Yan Zheng
    • Zhisong Shi
    • Guanghui Wang
  • View Affiliations

  • Published online on: February 23, 2022     https://doi.org/10.3892/ijo.2022.5328
  • Article Number: 38
  • Copyright: © Yu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Circular RNA‑lipoprotein receptor 6 (circ‑LRP6) serves a role in promoting the tumorigenesis of retinoblastoma, esophageal squamous cell cancer and oral squamous cell carcinoma; however, whether circ‑LRP6 demonstrates the same effect in osteosarcoma (OS) is yet to be fully elucidated. The present study aimed to analyze the expression, role and potential molecular mechanism of circ‑LRP6 in OS. The expression levels of circ‑LRP6, microRNA (miR)‑141‑3p, histone deacetylase 4 (HDAC4) and high mobility group protein 1 (HMGB1) were evaluated by reverse transcription-quantitative PCR in OS tissues and cell lines. Cell Counting Kit‑8, Transwell and Matrigel assays were conducted to evaluate cell proliferation, migration and invasion, respectively. Western blotting was also performed to determine HDAC4 and HMGB1 protein expression levels. Bioinformatics and dual‑luciferase reporter assays were used to predict and analyze the interactions between circ‑LRP6 and miR‑141‑3p, miR‑141‑3p and HDAC4, as well as between miR‑141‑3p and HMGB1. Additionally, RNA immunoprecipitation was performed to verify the association between circ‑LRP6 and miR‑141‑3p. The results confirmed that circ‑LRP6 was highly expressed in OS tissues and cell lines. In addition, circ‑LRP6 negatively regulated the expression of miR‑141‑3p and, in turn, miR‑141‑3p negatively regulated HDAC4 and HMGB1 expression. Functional assays revealed that circ‑LRP6 knockdown inhibited the proliferation, migration and invasion of OS cells, whereas the inhibition of miR‑141‑3p or the overexpression of either HDAC4 or HMGB1 partly reversed the inhibitory effect of circ‑LRP6 knockdown. In summary, the present study determined that circ‑LRP6 knockdown inhibited the proliferation, migration and invasion of OS cells by regulating the miR‑141‑3p/HDAC4/HMGB1 axis.
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April-2022
Volume 60 Issue 4

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Online ISSN:1791-2423

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Copy and paste a formatted citation
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Spandidos Publications style
Yu Y, Dong G, Li Z, Zheng Y, Shi Z and Wang G: circ‑LRP6 contributes to osteosarcoma progression by regulating the miR‑141‑3p/HDAC4/HMGB1 axis. Int J Oncol 60: 38, 2022
APA
Yu, Y., Dong, G., Li, Z., Zheng, Y., Shi, Z., & Wang, G. (2022). circ‑LRP6 contributes to osteosarcoma progression by regulating the miR‑141‑3p/HDAC4/HMGB1 axis. International Journal of Oncology, 60, 38. https://doi.org/10.3892/ijo.2022.5328
MLA
Yu, Y., Dong, G., Li, Z., Zheng, Y., Shi, Z., Wang, G."circ‑LRP6 contributes to osteosarcoma progression by regulating the miR‑141‑3p/HDAC4/HMGB1 axis". International Journal of Oncology 60.4 (2022): 38.
Chicago
Yu, Y., Dong, G., Li, Z., Zheng, Y., Shi, Z., Wang, G."circ‑LRP6 contributes to osteosarcoma progression by regulating the miR‑141‑3p/HDAC4/HMGB1 axis". International Journal of Oncology 60, no. 4 (2022): 38. https://doi.org/10.3892/ijo.2022.5328