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Ricolinostat enhances adavosertib‑induced mitotic catastrophe in TP53‑mutated head and neck squamous cell carcinoma cells

  • Authors:
    • Keitaro Miyake
    • Naoharu Takano
    • Hiromi Kazama
    • Hiroyuki Kikuchi
    • Masaki Hiramoto
    • Kiyoaki Tsukahara
    • Keisuke Miyazawa
  • View Affiliations / Copyright

    Affiliations: Department of Otorhinolaryngology, Head and Neck Surgery, Tokyo Medical University Hospital, Shinjuku‑ku, Tokyo 160‑0023, Japan, Department of Biochemistry, Tokyo Medical University, Shinjuku‑ku, Tokyo 160‑8402, Japan, Department of Preventive Medicine and Public Health, Tokyo Medical University, Shinjuku‑ku, Tokyo 160‑8402, Japan
    Copyright: © Miyake et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 4.0].
  • Article Number: 54
    |
    Published online on: March 28, 2022
       https://doi.org/10.3892/ijo.2022.5344
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Abstract

TP53 mutation is one of the most frequent gene mutations in head and neck squamous cell carcinoma (HNSCC) and could be a potential therapeutic target. Recently, the WEE1 G2 checkpoint kinase (WEE1) inhibitor adavosertib (Adv) has attracted attention because of its selective cytotoxicity against TP53‑mutated cells and has shown promising activity in early phase clinical trials. In the present study, it was demonstrated that combined treatment with Adv and a selective histone deacetylase 6 (HDAC6) inhibitor, ricolinostat (RCS), synergistically enhanced cell death induction in four out of five HNSCC cell lines with TP53 mutation (CAL27, SAS, HSC‑3, and OSC‑19), one HNSCC cell line with impaired TP53 function by HPV‑infection (UPCI‑SCC154), and TP53‑knockout human lung cancer cell line (A549 TP53‑KO), but not in TP53 wild‑type A549 cells. Time‑lapse imaging showed that RCS enhanced the Adv‑induced mitotic catastrophe. Consistent with this, RCS treatment suppressed checkpoint kinase 1 (Chk1) (Ser345) phosphorylation and co‑administration of RCS with Adv suppressed cyclin‑dependent kinase 1 (Tyr15) phosphorylation along with increased expression of γ‑H2A.X, a marker of DNA double‑strand breaks in CAL27 cells. These data showed that RCS enhanced Adv‑induced premature mitotic entry and cell death induction in the mitotic phase. However, although HDAC6 knockdown enhanced Adv‑induced cell death with γ‑H2A.X elevation, HDAC6 knockdown did not repress Chk1 phosphorylation in CAL27 cells. Our data demonstrated that the co‑administration of RCS with Adv in HNSCC cells resulted in the suppression of Chk1 activity, leading to synergistically enhanced apoptosis via mitotic catastrophe in a p53‑dependent manner. This enhanced cell death appeared to be partially mediated by the inhibition of HDAC6 activity by RCS.
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Spandidos Publications style
Miyake K, Takano N, Kazama H, Kikuchi H, Hiramoto M, Tsukahara K and Miyazawa K: Ricolinostat enhances adavosertib‑induced mitotic catastrophe in TP53‑mutated head and neck squamous cell carcinoma cells. Int J Oncol 60: 54, 2022.
APA
Miyake, K., Takano, N., Kazama, H., Kikuchi, H., Hiramoto, M., Tsukahara, K., & Miyazawa, K. (2022). Ricolinostat enhances adavosertib‑induced mitotic catastrophe in TP53‑mutated head and neck squamous cell carcinoma cells. International Journal of Oncology, 60, 54. https://doi.org/10.3892/ijo.2022.5344
MLA
Miyake, K., Takano, N., Kazama, H., Kikuchi, H., Hiramoto, M., Tsukahara, K., Miyazawa, K."Ricolinostat enhances adavosertib‑induced mitotic catastrophe in TP53‑mutated head and neck squamous cell carcinoma cells". International Journal of Oncology 60.5 (2022): 54.
Chicago
Miyake, K., Takano, N., Kazama, H., Kikuchi, H., Hiramoto, M., Tsukahara, K., Miyazawa, K."Ricolinostat enhances adavosertib‑induced mitotic catastrophe in TP53‑mutated head and neck squamous cell carcinoma cells". International Journal of Oncology 60, no. 5 (2022): 54. https://doi.org/10.3892/ijo.2022.5344
Copy and paste a formatted citation
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Spandidos Publications style
Miyake K, Takano N, Kazama H, Kikuchi H, Hiramoto M, Tsukahara K and Miyazawa K: Ricolinostat enhances adavosertib‑induced mitotic catastrophe in TP53‑mutated head and neck squamous cell carcinoma cells. Int J Oncol 60: 54, 2022.
APA
Miyake, K., Takano, N., Kazama, H., Kikuchi, H., Hiramoto, M., Tsukahara, K., & Miyazawa, K. (2022). Ricolinostat enhances adavosertib‑induced mitotic catastrophe in TP53‑mutated head and neck squamous cell carcinoma cells. International Journal of Oncology, 60, 54. https://doi.org/10.3892/ijo.2022.5344
MLA
Miyake, K., Takano, N., Kazama, H., Kikuchi, H., Hiramoto, M., Tsukahara, K., Miyazawa, K."Ricolinostat enhances adavosertib‑induced mitotic catastrophe in TP53‑mutated head and neck squamous cell carcinoma cells". International Journal of Oncology 60.5 (2022): 54.
Chicago
Miyake, K., Takano, N., Kazama, H., Kikuchi, H., Hiramoto, M., Tsukahara, K., Miyazawa, K."Ricolinostat enhances adavosertib‑induced mitotic catastrophe in TP53‑mutated head and neck squamous cell carcinoma cells". International Journal of Oncology 60, no. 5 (2022): 54. https://doi.org/10.3892/ijo.2022.5344
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