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Neural stem cell‑derived exosomes transfer miR‑124‑3p into cells to inhibit glioma growth by targeting FLOT2

  • Authors:
    • Cheng Qian
    • You Wang
    • Yunxiang Ji
    • Danmin Chen
    • Chuanfang Wang
    • Guilong Zhang
    • Yezhong Wang
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510260, P.R. China
    Copyright: © Qian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 115
    |
    Published online on: August 4, 2022
       https://doi.org/10.3892/ijo.2022.5405
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Abstract

Currently, exosomes (EXOs) are being explored as novel drug delivery carriers with greater advantages, including crossing the blood‑brain‑barrier and loading drugs. The present study utilized EXOs derived from neural stem cells (NSCs) for the delivery of molecular drugs to treat gliomas. miR‑124‑3p was selected according to previous studies by the authors, and the effects of the delivery of miR‑124‑3p to glioma cells by NSC‑EXOs in vitro and in vivo were evaluated. It was found that NSC‑EXOs successfully delivered miR‑124‑3p into glioma cells, and NSC‑EXOs loaded with miR‑124‑3p significantly inhibited glioma cell proliferation, invasion and migration. Furthermore, the delivery of miR‑124‑3p by NSC‑EXOs suppressed flotillin 2 (FLOT2) expression by specifically binding to the 3' untranslated region of the FLOT2 gene in gliomas; subsequently, AKT1 was found to be associated with the EXO‑miR‑124‑3p/FLOT2 pathway. Moreover, the therapeutic effects of the delivery of miR‑124‑3p by NSC‑EXOs were confirmed in a mouse tumor xenograft model of glioma. Thus, bio‑carrier NSC‑EXOs loaded with miR‑124‑3p suppressed glioma growth via the EXO‑miR‑124‑3p/FLOT2/AKT1 pathway. On the whole, the present study provides insight into stem cell‑free molecular‑targeted therapy based on bio‑carrier NSC‑EXOs and provides a potential strategy for the treatment of glioma.
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Copy and paste a formatted citation
Spandidos Publications style
Qian C, Wang Y, Ji Y, Chen D, Wang C, Zhang G and Wang Y: Neural stem cell‑derived exosomes transfer miR‑124‑3p into cells to inhibit glioma growth by targeting FLOT2. Int J Oncol 61: 115, 2022.
APA
Qian, C., Wang, Y., Ji, Y., Chen, D., Wang, C., Zhang, G., & Wang, Y. (2022). Neural stem cell‑derived exosomes transfer miR‑124‑3p into cells to inhibit glioma growth by targeting FLOT2. International Journal of Oncology, 61, 115. https://doi.org/10.3892/ijo.2022.5405
MLA
Qian, C., Wang, Y., Ji, Y., Chen, D., Wang, C., Zhang, G., Wang, Y."Neural stem cell‑derived exosomes transfer miR‑124‑3p into cells to inhibit glioma growth by targeting FLOT2". International Journal of Oncology 61.4 (2022): 115.
Chicago
Qian, C., Wang, Y., Ji, Y., Chen, D., Wang, C., Zhang, G., Wang, Y."Neural stem cell‑derived exosomes transfer miR‑124‑3p into cells to inhibit glioma growth by targeting FLOT2". International Journal of Oncology 61, no. 4 (2022): 115. https://doi.org/10.3892/ijo.2022.5405
Copy and paste a formatted citation
x
Spandidos Publications style
Qian C, Wang Y, Ji Y, Chen D, Wang C, Zhang G and Wang Y: Neural stem cell‑derived exosomes transfer miR‑124‑3p into cells to inhibit glioma growth by targeting FLOT2. Int J Oncol 61: 115, 2022.
APA
Qian, C., Wang, Y., Ji, Y., Chen, D., Wang, C., Zhang, G., & Wang, Y. (2022). Neural stem cell‑derived exosomes transfer miR‑124‑3p into cells to inhibit glioma growth by targeting FLOT2. International Journal of Oncology, 61, 115. https://doi.org/10.3892/ijo.2022.5405
MLA
Qian, C., Wang, Y., Ji, Y., Chen, D., Wang, C., Zhang, G., Wang, Y."Neural stem cell‑derived exosomes transfer miR‑124‑3p into cells to inhibit glioma growth by targeting FLOT2". International Journal of Oncology 61.4 (2022): 115.
Chicago
Qian, C., Wang, Y., Ji, Y., Chen, D., Wang, C., Zhang, G., Wang, Y."Neural stem cell‑derived exosomes transfer miR‑124‑3p into cells to inhibit glioma growth by targeting FLOT2". International Journal of Oncology 61, no. 4 (2022): 115. https://doi.org/10.3892/ijo.2022.5405
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