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Galectin‑1 binds GRP78 to promote the proliferation and metastasis of gastric cancer

  • Authors:
    • Qi Zhang
    • Muhammad Ali
    • Yang Wang
    • Qian-Nan Sun
    • Xiao-Dong Zhu
    • Dong Tang
    • Wei Wang
    • Cang-Yuan Zhang
    • Hai-Hua Zhou
    • Dao-Rong Wang
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Northern Jiangsu People's Hospital, Clinical Medical College, Yangzhou University, Yangzhou, Jiangsu 225001, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 141
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    Published online on: September 29, 2022
       https://doi.org/10.3892/ijo.2022.5431
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Abstract

The present study aimed to investigate the potential molecular mechanisms by which galectin‑1 (Gal‑1) and glucose‑regulated protein 78 (GRP78) influence the development of malignant gastric cancer (GC). Immunohistochemistry and western blotting were used to map the expression and location of the Gal‑1 gene in the 80 paraffin‑embedded GC samples, 16 fresh samples and surrounding tissues. Gal‑1 was overexpressed and knocked down using lentiviral vectors in the human GC cell lines HGC‑27 and AGS. Through the use of the Cell Counting Kit‑8 assay, clone formation assay, wound healing assay, invasion assay and tumor xenograft, the possible biological roles of Gal‑1 were further evaluated. The downstream interacting proteins were predicted by the BioGRID database, and GRP78 was chosen for further investigation. Immunofluorescence labeling and Co‑IP were used to confirm the connection. The statistical tests utilized were the two‑tailed paired Student's t‑test, χ2 test, Kaplan‑Meier and Cox regression analysis, and Spearman's rank correlation coefficients. In GC, Gal‑1 is extensively expressed and has the potential to interact with GRP78. Poor prognosis is linked to high levels of GRP78 and Gal‑1 expression in patients with GC. According to the functional study, Gal‑1 knockdown prevented cells from thriving and pushed Gal‑1 expression, which aided in the proliferation, migration and invasion of GC. Gal‑1 overexpression additionally aided the development of subcutaneous xenograft tumors. The mechanistic investigation proved that GRP78 and Gal‑1 interacted, accelerating the course of GC. Gal‑1 silencing had an inhibitory effect on the proliferation of HGC‑27 cells that was removed by ectopic GRP78 expression, whereas the stimulating effects of Gal‑1 overexpression in AGS cells were inhibited by GRP78 knockdown. In conclusion, Gal‑1 interacts with GRP78 to facilitate the advancement of GC. The Gal‑1/GRP78 axis is supported by the functional data of the present study as a possible GC treatment target.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang Q, Ali M, Wang Y, Sun Q, Zhu X, Tang D, Wang W, Zhang C, Zhou H, Wang D, Wang D, et al: Galectin‑1 binds GRP78 to promote the proliferation and metastasis of gastric cancer. Int J Oncol 61: 141, 2022.
APA
Zhang, Q., Ali, M., Wang, Y., Sun, Q., Zhu, X., Tang, D. ... Wang, D. (2022). Galectin‑1 binds GRP78 to promote the proliferation and metastasis of gastric cancer. International Journal of Oncology, 61, 141. https://doi.org/10.3892/ijo.2022.5431
MLA
Zhang, Q., Ali, M., Wang, Y., Sun, Q., Zhu, X., Tang, D., Wang, W., Zhang, C., Zhou, H., Wang, D."Galectin‑1 binds GRP78 to promote the proliferation and metastasis of gastric cancer". International Journal of Oncology 61.5 (2022): 141.
Chicago
Zhang, Q., Ali, M., Wang, Y., Sun, Q., Zhu, X., Tang, D., Wang, W., Zhang, C., Zhou, H., Wang, D."Galectin‑1 binds GRP78 to promote the proliferation and metastasis of gastric cancer". International Journal of Oncology 61, no. 5 (2022): 141. https://doi.org/10.3892/ijo.2022.5431
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Q, Ali M, Wang Y, Sun Q, Zhu X, Tang D, Wang W, Zhang C, Zhou H, Wang D, Wang D, et al: Galectin‑1 binds GRP78 to promote the proliferation and metastasis of gastric cancer. Int J Oncol 61: 141, 2022.
APA
Zhang, Q., Ali, M., Wang, Y., Sun, Q., Zhu, X., Tang, D. ... Wang, D. (2022). Galectin‑1 binds GRP78 to promote the proliferation and metastasis of gastric cancer. International Journal of Oncology, 61, 141. https://doi.org/10.3892/ijo.2022.5431
MLA
Zhang, Q., Ali, M., Wang, Y., Sun, Q., Zhu, X., Tang, D., Wang, W., Zhang, C., Zhou, H., Wang, D."Galectin‑1 binds GRP78 to promote the proliferation and metastasis of gastric cancer". International Journal of Oncology 61.5 (2022): 141.
Chicago
Zhang, Q., Ali, M., Wang, Y., Sun, Q., Zhu, X., Tang, D., Wang, W., Zhang, C., Zhou, H., Wang, D."Galectin‑1 binds GRP78 to promote the proliferation and metastasis of gastric cancer". International Journal of Oncology 61, no. 5 (2022): 141. https://doi.org/10.3892/ijo.2022.5431
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