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UBDP1 pseudogene and UBD network competitively bind miR‑6072 to promote glioma progression

  • Authors:
    • Fan Hong
    • Zhenyu Gong
    • Chao Chen
    • Tianzhen Hua
    • Qilin Huang
    • Yu'e Liu
    • Peipei Ma
    • Xu Zhang
    • Hongxiang Wang
    • Juxiang Chen
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Second Affiliated Hospital of Anhui Medical University, Anhui Medical University, Hefei, Anhui 230601, P.R. China, Department of Neurosurgery, Klinikum rechts der Isar, Technical University of Munich, D‑81675 Munich, Germany, Department of Neurosurgery, Changhai Hospital, Naval Medical University, Shanghai 200433, P.R. China, Tongji University Cancer Center, Shanghai Tenth People's Hospital of Tongji University, School of Medicine, Tongji University, Shanghai 200092, P.R. China
    Copyright: © Hong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 29
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    Published online on: January 22, 2024
       https://doi.org/10.3892/ijo.2024.5617
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Abstract

Increasing evidence suggests that pseudogenes play crucial roles in various cancers, yet their functions and regulatory mechanisms in glioma pathogenesis remain enigmatic. In the present study, a novel pseudogene was identified, UBDP1, which is significantly upregulated in glioblastoma and positively correlated with the expression of its parent gene, UBD. Additionally, high levels of these paired genes are linked with a poor prognosis for patients. In the present study, clinical samples were collected followed by various analyses including microarray for long non‑coding RNAs, reverse transcription‑quantitative PCR, fluorescence in situ hybridization and western blotting. Cell lines were authenticated and cultured then subjected to various assays for proliferation, migration, and invasion to investigate the molecular mechanisms. Bioinformatic tools identified miRNA targets, and luciferase reporter assays validated these interactions. A tumor xenograft model in mice was used for in vivo studies. In vitro and in vivo studies have demonstrated that UBDP1, localized in the cytoplasm, functions as a tumor‑promoting factor influencing cell proliferation, migration, invasion and tumor growth. Mechanistic investigations have indicated that UBDP1 exerts its oncogenic effects by decoying miR‑6072 from UBD mRNA, thus forming a competitive endogenous RNA network, which results in the enhanced oncogenic activity of UBD. The present findings offered new insights into the role of pseudogenes in glioma progression, suggesting that targeting the UBDP1/miR‑6072/UBD network may serve as a potential therapeutic strategy for glioma patients.
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Copy and paste a formatted citation
Spandidos Publications style
Hong F, Gong Z, Chen C, Hua T, Huang Q, Liu Y, Ma P, Zhang X, Wang H, Chen J, Chen J, et al: UBDP1 pseudogene and UBD network competitively bind miR‑6072 to promote glioma progression. Int J Oncol 64: 29, 2024.
APA
Hong, F., Gong, Z., Chen, C., Hua, T., Huang, Q., Liu, Y. ... Chen, J. (2024). UBDP1 pseudogene and UBD network competitively bind miR‑6072 to promote glioma progression. International Journal of Oncology, 64, 29. https://doi.org/10.3892/ijo.2024.5617
MLA
Hong, F., Gong, Z., Chen, C., Hua, T., Huang, Q., Liu, Y., Ma, P., Zhang, X., Wang, H., Chen, J."UBDP1 pseudogene and UBD network competitively bind miR‑6072 to promote glioma progression". International Journal of Oncology 64.3 (2024): 29.
Chicago
Hong, F., Gong, Z., Chen, C., Hua, T., Huang, Q., Liu, Y., Ma, P., Zhang, X., Wang, H., Chen, J."UBDP1 pseudogene and UBD network competitively bind miR‑6072 to promote glioma progression". International Journal of Oncology 64, no. 3 (2024): 29. https://doi.org/10.3892/ijo.2024.5617
Copy and paste a formatted citation
x
Spandidos Publications style
Hong F, Gong Z, Chen C, Hua T, Huang Q, Liu Y, Ma P, Zhang X, Wang H, Chen J, Chen J, et al: UBDP1 pseudogene and UBD network competitively bind miR‑6072 to promote glioma progression. Int J Oncol 64: 29, 2024.
APA
Hong, F., Gong, Z., Chen, C., Hua, T., Huang, Q., Liu, Y. ... Chen, J. (2024). UBDP1 pseudogene and UBD network competitively bind miR‑6072 to promote glioma progression. International Journal of Oncology, 64, 29. https://doi.org/10.3892/ijo.2024.5617
MLA
Hong, F., Gong, Z., Chen, C., Hua, T., Huang, Q., Liu, Y., Ma, P., Zhang, X., Wang, H., Chen, J."UBDP1 pseudogene and UBD network competitively bind miR‑6072 to promote glioma progression". International Journal of Oncology 64.3 (2024): 29.
Chicago
Hong, F., Gong, Z., Chen, C., Hua, T., Huang, Q., Liu, Y., Ma, P., Zhang, X., Wang, H., Chen, J."UBDP1 pseudogene and UBD network competitively bind miR‑6072 to promote glioma progression". International Journal of Oncology 64, no. 3 (2024): 29. https://doi.org/10.3892/ijo.2024.5617
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