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Article Open Access

circ_0004662 contributes to colorectal cancer progression by interacting with hnRNPM

  • Authors:
    • Yang Zhang
    • Jian Wang
    • Ruiliang Quan
    • Lihua Lyu
  • View Affiliations / Copyright

    Affiliations: Department of Laboratory Medicine, The First Affiliated Hospital of University of Science and Technology of China, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230031, P.R. China, Department of Hepatopancreatobiliary Surgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230031, P.R. China, Department of Gastrointestinal Surgery, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230031, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 14
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    Published online on: January 14, 2025
       https://doi.org/10.3892/ijo.2025.5720
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Abstract

Circular (circ)RNAs participate in colorectal cancer (CRC) occurrence and progression. However, the role of hsa_circ_0004662 (circ_0004662) in CRC remains unknown. Reverse transcription‑quantitative PCR noted high expression of circ_0004662 in CRC compared with normal colorectal epithelial cells. circ_0004662 knockdown inhibited migration of CRC cells in vitro and in vivo; would healing and Transwell assays showed that circ_0004662 overexpression contributed to CRC migration. Nuclear cytoplasmic analysis and fluorescence in situ hybridization revealed localization of circ_0004662 in the nucleus and cytoplasm. CircRNADB databases predicted that circ_0004662 exhibited translational potential and liquid chromatography‑mass spectrometry (LC‑MS) of circ_0004662 pull‑down products suggested that circ_0004662 bound to multiple ribosomal subunits. However, peptide products of 149aa translated by circ_0004662, with a molecular weight of ~17 kDa were not detected. Nevertheless, LC‑MS analysis indicated that circ_0004662 bound multiple proteins. Immunoprecipitation of RNA‑binding proteins revealed that circ_0004662 bound to heterogeneous nuclear ribonucleoprotein M (hnRNPM) and that hnRNPM interference decreased circ_0004662 expression, thereby affecting CRC progression. In summary, circ_0004662 was significantly upregulated in CRC. As a non‑coding RNA, it may promote CRC progression by binding to hnRNPM, which may serve as a potential target for treating CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang Y, Wang J, Quan R and Lyu L: circ_0004662 contributes to colorectal cancer progression by interacting with hnRNPM. Int J Oncol 66: 14, 2025.
APA
Zhang, Y., Wang, J., Quan, R., & Lyu, L. (2025). circ_0004662 contributes to colorectal cancer progression by interacting with hnRNPM. International Journal of Oncology, 66, 14. https://doi.org/10.3892/ijo.2025.5720
MLA
Zhang, Y., Wang, J., Quan, R., Lyu, L."circ_0004662 contributes to colorectal cancer progression by interacting with hnRNPM". International Journal of Oncology 66.2 (2025): 14.
Chicago
Zhang, Y., Wang, J., Quan, R., Lyu, L."circ_0004662 contributes to colorectal cancer progression by interacting with hnRNPM". International Journal of Oncology 66, no. 2 (2025): 14. https://doi.org/10.3892/ijo.2025.5720
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Y, Wang J, Quan R and Lyu L: circ_0004662 contributes to colorectal cancer progression by interacting with hnRNPM. Int J Oncol 66: 14, 2025.
APA
Zhang, Y., Wang, J., Quan, R., & Lyu, L. (2025). circ_0004662 contributes to colorectal cancer progression by interacting with hnRNPM. International Journal of Oncology, 66, 14. https://doi.org/10.3892/ijo.2025.5720
MLA
Zhang, Y., Wang, J., Quan, R., Lyu, L."circ_0004662 contributes to colorectal cancer progression by interacting with hnRNPM". International Journal of Oncology 66.2 (2025): 14.
Chicago
Zhang, Y., Wang, J., Quan, R., Lyu, L."circ_0004662 contributes to colorectal cancer progression by interacting with hnRNPM". International Journal of Oncology 66, no. 2 (2025): 14. https://doi.org/10.3892/ijo.2025.5720
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