Human cytomegalovirus in neoplastic cells of Epstein-Barr virus negative Hodgkin's disease

  • Authors:
    • Gaosheng Huang
    • Qingguo Yan
    • Zhe Wang
    • Xiequn Chen
    • Xiaohui Zhang
    • Ying Guo
    • Jian Jian Li
  • View Affiliations

  • Published online on: July 1, 2002     https://doi.org/10.3892/ijo.21.1.31
  • Pages: 31-36
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Abstract

Although Epstein-Barr virus (EBV) infection has been implicated in the development of a subset of Hodgkin's disease, the etiology of EBV-negative Hodgkin's disease remains to be determined. We investigated the status of human cytomegalovirus (hCMV) infection in 42 cases of Hodgkin's disease in a Chinese population. Using a nested polymerase chain reaction assay, nine of the 42 cases (21.4%) were found to contain EBV DNA. While only one of the nine EBV-positive cases demonstrated amplifiable hCMV DNA, approximately 33.3% of the EBV-negative cases (11/33) showed molecular evidence of hCMV infection (8 mixed cellularity; 2 nodular sclerosis; and 1 lymphocyte depletion). All 17 lymph nodes with non-specific reactive changes were negative for hCMV DNA. The presence of hCMV in Reed-Sternberg cells and their variants was further confirmed by single-cell polymerase chain reaction, in situ hybridization and immunohistochemical analysis, which demonstrated ~50% amplified hCMV DNA. These results suggest for the first time that hCMV infection plays a potential role in signaling the cell transformation and pathogenesis of Hodgkin's disease, particularly in EBV-negative cases.

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July 2002
Volume 21 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Copy and paste a formatted citation
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Spandidos Publications style
Huang G, Yan Q, Wang Z, Chen X, Zhang X, Guo Y and Li JJ: Human cytomegalovirus in neoplastic cells of Epstein-Barr virus negative Hodgkin's disease. Int J Oncol 21: 31-36, 2002
APA
Huang, G., Yan, Q., Wang, Z., Chen, X., Zhang, X., Guo, Y., & Li, J.J. (2002). Human cytomegalovirus in neoplastic cells of Epstein-Barr virus negative Hodgkin's disease. International Journal of Oncology, 21, 31-36. https://doi.org/10.3892/ijo.21.1.31
MLA
Huang, G., Yan, Q., Wang, Z., Chen, X., Zhang, X., Guo, Y., Li, J. J."Human cytomegalovirus in neoplastic cells of Epstein-Barr virus negative Hodgkin's disease". International Journal of Oncology 21.1 (2002): 31-36.
Chicago
Huang, G., Yan, Q., Wang, Z., Chen, X., Zhang, X., Guo, Y., Li, J. J."Human cytomegalovirus in neoplastic cells of Epstein-Barr virus negative Hodgkin's disease". International Journal of Oncology 21, no. 1 (2002): 31-36. https://doi.org/10.3892/ijo.21.1.31