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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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August 2002 Volume 21 Issue 2

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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August 2002 Volume 21 Issue 2

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Article

CYFRA 21-1 is released in TNF-α-induced apoptosis in the hepatocellular carcinoma cell line HuH-7

  • Authors:
    • Fei Wu
    • Jiro Fujita
    • Masayuki Murota
    • Jia-Qing Li
    • Toshihiko Ishida
    • Mikio Nishioka
    • Yuji Imaida
    • Shigeki Kuriyama
  • View Affiliations / Copyright

    Affiliations: Third Department of Internal Medicine, Kagawa Medical University, Kagawa, Japan
  • Pages: 441-445
    |
    Published online on: August 1, 2002
       https://doi.org/10.3892/ijo.21.2.441
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Abstract

Many types of cancer cells widely express cytokeratin 19 (CK19). Clinical investigations have suggested that serum CYFRA 21-1, a fragment of CK19, is one of the most useful tumor markers. In the present study, we hypothesized that released CYFRA 21-1 is closely associated with cellular apoptosis during tumor growth. Apoptosis was induced by tumor necrosis factor-α (TNF-α) in the HuH-7 hepatocellular carcinoma cell line. Both TNF-α-treated and non-treated cells of HuH-7 were simultaneously examined by immunoradiometric assay, annexin-V apoptosis analysis, immunohistochemical staining and colorimetric protease measurement. Levels of CYFRA 21-1 increased significantly in TNF-α-treated cells displaying a high percentage of apoptosis, granular-like aggregation of CK19, and elevated activity of caspase-3 in contrast to non-treated cells. Levels of CYFRA 21-1 decreased significantly after caspase-3 was inhibited in TNF-α-treated cells. Thus, the release of CYFRA 21-1 may suggest cellular apoptosis in the process of tumor growth.

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Copy and paste a formatted citation
Spandidos Publications style
Wu F, Fujita J, Murota M, Li J, Ishida T, Nishioka M, Imaida Y and Kuriyama S: CYFRA 21-1 is released in TNF-α-induced apoptosis in the hepatocellular carcinoma cell line HuH-7. Int J Oncol 21: 441-445, 2002.
APA
Wu, F., Fujita, J., Murota, M., Li, J., Ishida, T., Nishioka, M. ... Kuriyama, S. (2002). CYFRA 21-1 is released in TNF-α-induced apoptosis in the hepatocellular carcinoma cell line HuH-7. International Journal of Oncology, 21, 441-445. https://doi.org/10.3892/ijo.21.2.441
MLA
Wu, F., Fujita, J., Murota, M., Li, J., Ishida, T., Nishioka, M., Imaida, Y., Kuriyama, S."CYFRA 21-1 is released in TNF-α-induced apoptosis in the hepatocellular carcinoma cell line HuH-7". International Journal of Oncology 21.2 (2002): 441-445.
Chicago
Wu, F., Fujita, J., Murota, M., Li, J., Ishida, T., Nishioka, M., Imaida, Y., Kuriyama, S."CYFRA 21-1 is released in TNF-α-induced apoptosis in the hepatocellular carcinoma cell line HuH-7". International Journal of Oncology 21, no. 2 (2002): 441-445. https://doi.org/10.3892/ijo.21.2.441
Copy and paste a formatted citation
x
Spandidos Publications style
Wu F, Fujita J, Murota M, Li J, Ishida T, Nishioka M, Imaida Y and Kuriyama S: CYFRA 21-1 is released in TNF-α-induced apoptosis in the hepatocellular carcinoma cell line HuH-7. Int J Oncol 21: 441-445, 2002.
APA
Wu, F., Fujita, J., Murota, M., Li, J., Ishida, T., Nishioka, M. ... Kuriyama, S. (2002). CYFRA 21-1 is released in TNF-α-induced apoptosis in the hepatocellular carcinoma cell line HuH-7. International Journal of Oncology, 21, 441-445. https://doi.org/10.3892/ijo.21.2.441
MLA
Wu, F., Fujita, J., Murota, M., Li, J., Ishida, T., Nishioka, M., Imaida, Y., Kuriyama, S."CYFRA 21-1 is released in TNF-α-induced apoptosis in the hepatocellular carcinoma cell line HuH-7". International Journal of Oncology 21.2 (2002): 441-445.
Chicago
Wu, F., Fujita, J., Murota, M., Li, J., Ishida, T., Nishioka, M., Imaida, Y., Kuriyama, S."CYFRA 21-1 is released in TNF-α-induced apoptosis in the hepatocellular carcinoma cell line HuH-7". International Journal of Oncology 21, no. 2 (2002): 441-445. https://doi.org/10.3892/ijo.21.2.441
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