Molecular and genetic characterization of a non-metastatic human esophageal cancer cell line, T.Tn expressing non-functional mutated p53

  • Authors:
    • T. Sakai
    • T. Furihata
    • H. Kawamata
    • F. Omotehara
    • Y. Shinagawa
    • J. Imura
    • K. Kubota
    • A. Terano
    • T. Fujimori
  • View Affiliations

  • Published online on: September 1, 2002     https://doi.org/10.3892/ijo.21.3.547
  • Pages: 547-552
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Abstract

Lymph node metastasis is commonly found in esophageal squamous cell carcinoma (SCC). In this study, we examined the molecular and genetic characteristics of a human esophageal SCC cell line, T.Tn. T.Tn cells formed tumors at s.c. tissue in nude mice when inoculated with Matrigel®, but did not metastasize to any organs. T.Tn cells expressed low level of proMMP2 and a trace level of proMMP9. However, T.Tn cells expressed high level of TIMP1 and TIMP2, and β-catenin and E-cadherin. We found a point mutation of p53 gene at codon 213 (CAT↷CGT) in T.Tn cells. The mutated-p53 protein did not show transcriptional activity on p21waf1, MDM2 and Bax promoters. Thus, T.Tn cells are low tumorigenic and weakly invasive but not metastasizing in nude mice, and T.Tn cells are suitable parental cells for establishing a model system to study invasion and metastasis of esophageal SCC.

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September 2002
Volume 21 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Sakai T, Furihata T, Kawamata H, Omotehara F, Shinagawa Y, Imura J, Kubota K, Terano A and Fujimori T: Molecular and genetic characterization of a non-metastatic human esophageal cancer cell line, T.Tn expressing non-functional mutated p53. Int J Oncol 21: 547-552, 2002
APA
Sakai, T., Furihata, T., Kawamata, H., Omotehara, F., Shinagawa, Y., Imura, J. ... Fujimori, T. (2002). Molecular and genetic characterization of a non-metastatic human esophageal cancer cell line, T.Tn expressing non-functional mutated p53. International Journal of Oncology, 21, 547-552. https://doi.org/10.3892/ijo.21.3.547
MLA
Sakai, T., Furihata, T., Kawamata, H., Omotehara, F., Shinagawa, Y., Imura, J., Kubota, K., Terano, A., Fujimori, T."Molecular and genetic characterization of a non-metastatic human esophageal cancer cell line, T.Tn expressing non-functional mutated p53". International Journal of Oncology 21.3 (2002): 547-552.
Chicago
Sakai, T., Furihata, T., Kawamata, H., Omotehara, F., Shinagawa, Y., Imura, J., Kubota, K., Terano, A., Fujimori, T."Molecular and genetic characterization of a non-metastatic human esophageal cancer cell line, T.Tn expressing non-functional mutated p53". International Journal of Oncology 21, no. 3 (2002): 547-552. https://doi.org/10.3892/ijo.21.3.547