Somatic mutation and SNP in the promoter of dbpA and human hepatocarcinogenesis

  • Authors:
    • Junpei Hayashi
    • Kazunori Kajino
    • Tomoyuki Umeda
    • Seigo Takano
    • Yasuyuki Arakawa
    • Masatoshi Kudo
    • Okio Hino
  • View Affiliations

  • Published online on: October 1, 2002     https://doi.org/10.3892/ijo.21.4.847
  • Pages: 847-850
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Human DNA-binding protein (dbpA) is a member of a Y-box binding protein family containing a cold shock domain. The increased expression of Y box binding proteins in somatic cells is associated with cell proliferation and transformation. Recently, we isolated a splicing variant of dbpA as a candidate for the cellular recombinogenic protein that leads to genomic instability and inflammation-mediated hepatocarcinogenesis. The expression of dbpA is enhanced in proliferating cells, but the manner in which it regulates transcription is largely unknown. In this study, we analyzed the transcriptional regulatory region of dbpA, and searched for the mutation in this region by a direct sequence method. In 3 of 55 human hepatocellular carcinoma (HCC) cases, we identified one nucleotide replacement (T↷G transversion) in nucleotide position -6 of the promoter region. Among 3 cases showing this transversion, one HCC case was due to a somatic mutation and the other two were due to single nucleotide polymorphism (SNP). By luciferase assay, we showed that the transcriptional activity of the promoter region with the transversion was significantly higher than that of the wild-type. Using the Southwestern blotting, we also confirmed the existence of a cellular proteins (about 25 and 50 kDa) that specifically bind to the sequence with this transversion. Our results suggested the biological significance of the transversion of dbpA's promoter region as one of the factors accelerating hepatocarcinogenesis.

Related Articles

Journal Cover

October 2002
Volume 21 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Hayashi J, Kajino K, Umeda T, Takano S, Arakawa Y, Kudo M and Hino O: Somatic mutation and SNP in the promoter of dbpA and human hepatocarcinogenesis. Int J Oncol 21: 847-850, 2002
APA
Hayashi, J., Kajino, K., Umeda, T., Takano, S., Arakawa, Y., Kudo, M., & Hino, O. (2002). Somatic mutation and SNP in the promoter of dbpA and human hepatocarcinogenesis. International Journal of Oncology, 21, 847-850. https://doi.org/10.3892/ijo.21.4.847
MLA
Hayashi, J., Kajino, K., Umeda, T., Takano, S., Arakawa, Y., Kudo, M., Hino, O."Somatic mutation and SNP in the promoter of dbpA and human hepatocarcinogenesis". International Journal of Oncology 21.4 (2002): 847-850.
Chicago
Hayashi, J., Kajino, K., Umeda, T., Takano, S., Arakawa, Y., Kudo, M., Hino, O."Somatic mutation and SNP in the promoter of dbpA and human hepatocarcinogenesis". International Journal of Oncology 21, no. 4 (2002): 847-850. https://doi.org/10.3892/ijo.21.4.847