Genetic alterations in adenoma-carcinoma sequencing of intraductal papillary-mucinous neoplasm of the pancreas

  • Authors:
    • B. Nakata
    • M. Yashiro
    • N. Nishioka
    • M. Aya
    • S. Yamada
    • C. Takenaka
    • M. Ohira
    • T. Ishikawa
    • H. Nishino
    • K. Wakasa
    • K. Hirakawa
  • View Affiliations

  • Published online on: November 1, 2002     https://doi.org/10.3892/ijo.21.5.1067
  • Pages: 1067-1072
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Abstract

We investigated the adenoma-carcinoma sequence in intraductal papillary-mucinous neoplasm from the aspect of genetic changes. The formalin-fixed paraffin-embedded tumors and surrounding normal pancreatic tissues from patients with 16 intraductal papillary-mucinous adenoma of the pancreas (IPMA) and 10 intraductal papillary-mucinous carcinoma of the pancreas (IPMC) were provided for DNA extraction after microdissection. SSCP-DNA sequencing analysis demonstrated K-ras mutations at codon 12 in 75% of IPMA and 70% of IPMC, while those at codon 13 were observed neither in IPMA nor IPMC. There were no characteristic K-ras mutation types in IPMA and IPMC and no significant differences in incidence of K-ras mutations between the two categories. The frequencies of p53 mutations analyzed by SSCP-DNA sequencing were not high in IPMA (18.8%) and IPMC (30%), showing no significant difference between them. LOHs of APC in IPMA and IPMC were infrequent (6.3 and 20%, respectively) and showed no significant difference in incidence between the two categories. The LOH frequencies of DCC in IPMA and IPMC were 31.3 and 40%, respectively, and were not statistically different from each other. Taken together, genetic changes such as K-ras, p53, APC and DCC mutations may not be associated with adenoma-carcinoma sequence in intraductal papillary-mucinous neoplasm of pancreas.

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November 2002
Volume 21 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Nakata B, Yashiro M, Nishioka N, Aya M, Yamada S, Takenaka C, Ohira M, Ishikawa T, Nishino H, Wakasa K, Wakasa K, et al: Genetic alterations in adenoma-carcinoma sequencing of intraductal papillary-mucinous neoplasm of the pancreas. Int J Oncol 21: 1067-1072, 2002
APA
Nakata, B., Yashiro, M., Nishioka, N., Aya, M., Yamada, S., Takenaka, C. ... Hirakawa, K. (2002). Genetic alterations in adenoma-carcinoma sequencing of intraductal papillary-mucinous neoplasm of the pancreas. International Journal of Oncology, 21, 1067-1072. https://doi.org/10.3892/ijo.21.5.1067
MLA
Nakata, B., Yashiro, M., Nishioka, N., Aya, M., Yamada, S., Takenaka, C., Ohira, M., Ishikawa, T., Nishino, H., Wakasa, K., Hirakawa, K."Genetic alterations in adenoma-carcinoma sequencing of intraductal papillary-mucinous neoplasm of the pancreas". International Journal of Oncology 21.5 (2002): 1067-1072.
Chicago
Nakata, B., Yashiro, M., Nishioka, N., Aya, M., Yamada, S., Takenaka, C., Ohira, M., Ishikawa, T., Nishino, H., Wakasa, K., Hirakawa, K."Genetic alterations in adenoma-carcinoma sequencing of intraductal papillary-mucinous neoplasm of the pancreas". International Journal of Oncology 21, no. 5 (2002): 1067-1072. https://doi.org/10.3892/ijo.21.5.1067