Immunodetection and molecular forms of plasma vascular endothelial growth factor-C

  • Authors:
    • S. E. Duff
    • C. Li
    • A. Renehan
    • S. T. O'Dwyer
    • S. Kumar
  • View Affiliations

  • Published online on: February 1, 2003     https://doi.org/10.3892/ijo.22.2.339
  • Pages: 339-343
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Abstract

Vascular endothelial growth factor (VEGF)-C is a member of the VEGF family. VEGF-C is involved in developmental lymphangiogenesis and may be important in pathological lymphangiogenesis, lymphatic invasion and metastasis in carcinoma. We describe the development of an indirect enzyme-linked immunosorbent (ELISA) assay for the quantification of VEGF-C in plasma. Capture of VEGF-C was achieved using goat anti-human VEGF-C antibody, followed by detection with rabbit anti-human VEGF-C antibody. The sensitivity of the assay was amplified using the biotin-avidin and enhanced chemiluminescence (ECL) systems. The assay was highly sensitive and reproducible with a detection range of 0.4-100 U/ml and the intra- and inter-assay variations were less than 8%. Substitutional tests demonstrated that the assay was specific for VEGF-C with no cross-reaction with VEGF-A or VEGF-D. Practical application of the assay was evaluated in 41 colorectal cancer patients and 31 controls. Median plasma levels of VEGF-C were 35.0 U/ml (range: 17.4-75.9 U/ml) in colorectal cancer patients in contrast to 11.5 U/ml (range: 5.4-21.5 U/ml) in controls (p<0.001). Moreover, VEGF-C levels tended to be elevated in patients with advanced disease compared to early disease, but this was not statistically significant owing to a relatively small number of patients in each group. Immunoprecipitation and immunoblotting confirmed detection of VEGF-C in plasma and revealed that two forms of VEGF-C were present in the plasma corresponding to ≈40 and ≈80 kDa. The measurement of plasma VEGF-C offers opportunities to explore clinical applications in the management of malignancy, in particular in the prediction of lymphatic spread and in other lymphangiogenesis-related diseases.

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February 2003
Volume 22 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Duff SE, Li C, Renehan A, O'Dwyer ST and Kumar S: Immunodetection and molecular forms of plasma vascular endothelial growth factor-C. Int J Oncol 22: 339-343, 2003
APA
Duff, S.E., Li, C., Renehan, A., O'Dwyer, S.T., & Kumar, S. (2003). Immunodetection and molecular forms of plasma vascular endothelial growth factor-C. International Journal of Oncology, 22, 339-343. https://doi.org/10.3892/ijo.22.2.339
MLA
Duff, S. E., Li, C., Renehan, A., O'Dwyer, S. T., Kumar, S."Immunodetection and molecular forms of plasma vascular endothelial growth factor-C". International Journal of Oncology 22.2 (2003): 339-343.
Chicago
Duff, S. E., Li, C., Renehan, A., O'Dwyer, S. T., Kumar, S."Immunodetection and molecular forms of plasma vascular endothelial growth factor-C". International Journal of Oncology 22, no. 2 (2003): 339-343. https://doi.org/10.3892/ijo.22.2.339