Monensin-mediated growth inhibition of SNU-C1 colon cancer cells via cell cycle arrest and apoptosis

  • Authors:
    • Woo Hyun Park
    • Eun Shil Kim
    • Chul Won Jung
    • Byoung Kook Kim
    • Young Yiul Lee
  • View Affiliations

  • Published online on: February 1, 2003     https://doi.org/10.3892/ijo.22.2.377
  • Pages: 377-382
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Abstract

Previously, we showed that monensin, Na+ ionophore, potently inhibited the growth of acute myelogenous leukemia and lymphoma cells. Here, we demonstrate that monensin inhibited the proliferation of solid tumor cells with IC50 of about 2.5 µM. Monensin induced a G1 or a G2-M phase arrest in these cells. When we examined the effects of this drug on SNU-C1 cells, monensin decreased the levels of CDK2, CDK4, CDK6, cyclin D1 and cyclin A proteins. While p27 was increased by monensin, p21 was not. In addition, monensin markedly enhanced the binding of p27 with CDK2, CDK4 and CDK6. Furthermore, the activities of CDK2-, CDK4- and CDK6-associated kinase were reduced in association with hypophosphorylation of Rb protein. Monensin also induced apoptosis in solid tumor cells. Apoptotic process of SNU-C1 cells was associated with the changes of Bax, caspase-3 and mitochondria transmembrane potential (ΔΨm). Taken together, these results demonstrated for the first time that monensin inhibited the growth of solid tumor cells, especially SNU-C1 cells, via cell cycle arrest and apoptosis.

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February 2003
Volume 22 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Park WH, Kim ES, Jung CW, Kim BK and Lee YY: Monensin-mediated growth inhibition of SNU-C1 colon cancer cells via cell cycle arrest and apoptosis. Int J Oncol 22: 377-382, 2003
APA
Park, W.H., Kim, E.S., Jung, C.W., Kim, B.K., & Lee, Y.Y. (2003). Monensin-mediated growth inhibition of SNU-C1 colon cancer cells via cell cycle arrest and apoptosis. International Journal of Oncology, 22, 377-382. https://doi.org/10.3892/ijo.22.2.377
MLA
Park, W. H., Kim, E. S., Jung, C. W., Kim, B. K., Lee, Y. Y."Monensin-mediated growth inhibition of SNU-C1 colon cancer cells via cell cycle arrest and apoptosis". International Journal of Oncology 22.2 (2003): 377-382.
Chicago
Park, W. H., Kim, E. S., Jung, C. W., Kim, B. K., Lee, Y. Y."Monensin-mediated growth inhibition of SNU-C1 colon cancer cells via cell cycle arrest and apoptosis". International Journal of Oncology 22, no. 2 (2003): 377-382. https://doi.org/10.3892/ijo.22.2.377