Methylation of promoter region in p27 gene plays a role in the development of lymphoid malignancies

  • Authors:
    • Shin-Ichi Nakatsuka
    • Angen Liu
    • Masayuki Yao
    • Tetsuya Takakuwa
    • Yasuhiko Tomita
    • Yoshihiko Hoshida
    • Mieko Nishiu
    • Katsuyuki Aozasa
  • View Affiliations

  • Published online on: March 1, 2003     https://doi.org/10.3892/ijo.22.3.561
  • Pages: 561-568
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

p27 belong to the cyclin-dependent kinase inhibitor family and plays an important role in regulation of cell cycle progression. Expression of these genes is epigenetically suppressed by methylation of their promoter regions. In this study, we examined protein expression and methylation status of the promoter region of p27 in 70 cases with non-Hodgkin's lymphoma and their relationship with proliferative activity of the tumor cells as revealed by Ki-67 index. There were 35 cases of B-cell, 11 of T-cell, 23 of NK/T-cell lymphoma, and 1 undetermined type. Immunohistochemically the loss of p27 protein expression was observed in 24 (69%) of 35 cases. Significant inverse correlation between p27 protein expression and Ki-67 index was found. Single strand conformation polymorphism (SSCP) followed by sequencing could not detect point mutations in any of the 68 cases. Bisulfite sequencing analysis showed that methylation of the promoter region of p27 were observed in 17 (25%) of 68 cases, in that several specific CpG sites around the start codon of p27 gene were preferentially methylated. Eight of 22 cases with loss of p27 protein expression showed methylation in CpG island of 5' region of p27 gene. These findings suggested that gene methylation plays a role in loss of expression of p27, which gives the cells proliferative advantages.

Related Articles

Journal Cover

March 2003
Volume 22 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Nakatsuka S, Liu A, Yao M, Takakuwa T, Tomita Y, Hoshida Y, Nishiu M and Aozasa K: Methylation of promoter region in p27 gene plays a role in the development of lymphoid malignancies. Int J Oncol 22: 561-568, 2003
APA
Nakatsuka, S., Liu, A., Yao, M., Takakuwa, T., Tomita, Y., Hoshida, Y. ... Aozasa, K. (2003). Methylation of promoter region in p27 gene plays a role in the development of lymphoid malignancies. International Journal of Oncology, 22, 561-568. https://doi.org/10.3892/ijo.22.3.561
MLA
Nakatsuka, S., Liu, A., Yao, M., Takakuwa, T., Tomita, Y., Hoshida, Y., Nishiu, M., Aozasa, K."Methylation of promoter region in p27 gene plays a role in the development of lymphoid malignancies". International Journal of Oncology 22.3 (2003): 561-568.
Chicago
Nakatsuka, S., Liu, A., Yao, M., Takakuwa, T., Tomita, Y., Hoshida, Y., Nishiu, M., Aozasa, K."Methylation of promoter region in p27 gene plays a role in the development of lymphoid malignancies". International Journal of Oncology 22, no. 3 (2003): 561-568. https://doi.org/10.3892/ijo.22.3.561