Mutational analysis of the nf2 tumour suppressor gene in three subtypes of primary human malignant mesotheliomas

  • Authors:
    • Holger Schipper
    • Thilo Papp
    • Georg Johnen
    • Heidi Pemsel
    • Ralf Bastrop
    • Klaus-Michael Müller
    • Thorsten Wiethege
    • Malgorzata Jaworska
    • Michael Krismann
    • Dietmar Schiffmann
    • Qamar Rahman
  • View Affiliations

  • Published online on: May 1, 2003     https://doi.org/10.3892/ijo.22.5.1009
  • Pages: 1009-1017
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Abstract

Fourteen primary human malignant mesothelioma (HMM) samples obtained from 14 patients were screened for point mutations and microdeletions/microinsertions in exons 1-16 of the chromosome 22q-located tumour suppressor gene neurofibromin 2 (nf2) by single strand conformation polymorphism (SSCP) analysis. In one tumour (7%) a 10 basepair microdeletion of exon 10 was detected by SSCP and subsequently characterised in detail by sequencing. Deletion of the second nf2 allele in laser-microdissected regions of the 10 bp mutation-harbouring tumour was demonstrated by denaturing gradient gel electrophoresis (DGGE) analysis. Simultaneous comparative genomic hybridisation (CGH) analysis also showed losses at chromosome 22q. Our data indicate that functional loss of the NF2 protein may be involved in the formation of a subset of HMMs.

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May 2003
Volume 22 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Schipper H, Papp T, Johnen G, Pemsel H, Bastrop R, Müller K, Wiethege T, Jaworska M, Krismann M, Schiffmann D, Schiffmann D, et al: Mutational analysis of the nf2 tumour suppressor gene in three subtypes of primary human malignant mesotheliomas. Int J Oncol 22: 1009-1017, 2003
APA
Schipper, H., Papp, T., Johnen, G., Pemsel, H., Bastrop, R., Müller, K. ... Rahman, Q. (2003). Mutational analysis of the nf2 tumour suppressor gene in three subtypes of primary human malignant mesotheliomas. International Journal of Oncology, 22, 1009-1017. https://doi.org/10.3892/ijo.22.5.1009
MLA
Schipper, H., Papp, T., Johnen, G., Pemsel, H., Bastrop, R., Müller, K., Wiethege, T., Jaworska, M., Krismann, M., Schiffmann, D., Rahman, Q."Mutational analysis of the nf2 tumour suppressor gene in three subtypes of primary human malignant mesotheliomas". International Journal of Oncology 22.5 (2003): 1009-1017.
Chicago
Schipper, H., Papp, T., Johnen, G., Pemsel, H., Bastrop, R., Müller, K., Wiethege, T., Jaworska, M., Krismann, M., Schiffmann, D., Rahman, Q."Mutational analysis of the nf2 tumour suppressor gene in three subtypes of primary human malignant mesotheliomas". International Journal of Oncology 22, no. 5 (2003): 1009-1017. https://doi.org/10.3892/ijo.22.5.1009