RB-resistant Abl kinase induces delayed cell cycle progression and increases susceptibility to apoptosis upon cellular stresses through interaction with p53

  • Authors:
    • Jae-We Cho
    • Junah Chung
    • Won-Ki Baek
    • Seong-Il Suh
    • Taeg Kyu Kwon
    • Jong-Wook Park
    • Min-Ho Suh
  • View Affiliations

  • Published online on: June 1, 2003     https://doi.org/10.3892/ijo.22.6.1193
  • Pages: 1193-1199
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

c-Abl, a non-receptor tyrosine kinase, is found in both nucleus and cytoplasm of proliferating fibroblasts. RB negatively regulates the kinase activity of c-Abl. Overexpression of kinase active c-Abl can overcome RB-induced growth arrest in Saos-2 cells. However, we previously reported that disruption of the RB matchmaker function leads to delayed cell cycle progression in the presence of p53. In this study, we investigated whether overexpression of mutant c-Abl (AS2, RB-resistant Abl kinase) not only lead to delayed cell cycle progression but also make cells susceptible to apoptosis under coexpression with a fragment of RB C pocket in human skin fibroblast. AS2 expressing cells showed delayed cell growth rate in normal growth condition. After genotoxic stress such as etoposide treatment, AS2 expressing cells readily progressed into apoptosis through p53 and caspase-3 activations. Our results suggest that expression of AS2 not only induces delayed cell cycle progression but also results in increased sensitivity to apoptosis in the presence of p53.

Related Articles

Journal Cover

June 2003
Volume 22 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Cho J, Chung J, Baek W, Suh S, Kwon TK, Park J and Suh M: RB-resistant Abl kinase induces delayed cell cycle progression and increases susceptibility to apoptosis upon cellular stresses through interaction with p53. Int J Oncol 22: 1193-1199, 2003
APA
Cho, J., Chung, J., Baek, W., Suh, S., Kwon, T.K., Park, J., & Suh, M. (2003). RB-resistant Abl kinase induces delayed cell cycle progression and increases susceptibility to apoptosis upon cellular stresses through interaction with p53. International Journal of Oncology, 22, 1193-1199. https://doi.org/10.3892/ijo.22.6.1193
MLA
Cho, J., Chung, J., Baek, W., Suh, S., Kwon, T. K., Park, J., Suh, M."RB-resistant Abl kinase induces delayed cell cycle progression and increases susceptibility to apoptosis upon cellular stresses through interaction with p53". International Journal of Oncology 22.6 (2003): 1193-1199.
Chicago
Cho, J., Chung, J., Baek, W., Suh, S., Kwon, T. K., Park, J., Suh, M."RB-resistant Abl kinase induces delayed cell cycle progression and increases susceptibility to apoptosis upon cellular stresses through interaction with p53". International Journal of Oncology 22, no. 6 (2003): 1193-1199. https://doi.org/10.3892/ijo.22.6.1193