Protease-activated receptor-2 expression and the role of trypsin in cell proliferation in human pancreatic cancers

Ohta,Tetsuo; Shimizu,Koichi; Yi,Shuangqin; Takamura,Hiroyuki; Amaya,Kohji; Kitagawa,Hirohisa; Kayahara,Masato; Ninomiya,Itasu; Fushida,Sachio; Fujimura,Takashi; Nishimura,Gen-Ichi; Miwa,Koichi

doi:10.3892/ijo.23.1.61

July 2003 Volume 23 Issue 1

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July 2003 Volume 23 Issue 1

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Article

Protease-activated receptor-2 expression and the role of trypsin in cell proliferation in human pancreatic cancers

Authors:
- Tetsuo Ohta
- Koichi Shimizu
- Shuangqin Yi
- Hiroyuki Takamura
- Kohji Amaya
- Hirohisa Kitagawa
- Masato Kayahara
- Itasu Ninomiya
- Sachio Fushida
- Takashi Fujimura
- Gen-Ichi Nishimura
- Koichi Miwa
View Affiliations / Copyright

Affiliations: Department of Gastroenterologic Surgery, Kanazawa University Hospital, Kanazawa 920-0934, Japan. ohtat@surg2.m.kanazawa-u.ac.jp
Pages: 61-66
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Published online on: July 1, 2003

https://doi.org/10.3892/ijo.23.1.61
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Abstract

Protease-activated receptor (PAR)-2 is a G protein-coupled receptor that is activated by trypsin. The purpose of this study was to examine PAR-2 expression and the role of trypsin in cell proliferation in human pancreatic cancer cells. All four pancreatic cancer cell lines studied, from well to undifferentiated types, AsPC-1, BxPC-3, Panc-1, and MIAPaCa-2, had significant levels of PAR-2 mRNA detected by reverse transcription-polymerase chain reaction, and showed a band of about 55 kDa corresponding to the known molecular weight of PAR-2: AsPC-1, BxPC-3 and Panc-1 showed a strong band, and MIAPaCa-2 showed a weak one. Immunocytochemically, AsPC-1, BxPC-3, and Panc-1 showed intense immunostaining for PAR-2, predominantly in the plasma membrane, while in MIAPaCa-2, immunostaining was weak. Proliferative activity of AsPC-1 cells was increased by concentrations of trypsin as low as 10 nM, and activity peaked at a concentration of 100 nM, representing almost 60% of that induced by 10% fetal bovine serum. In contrast, trypsin had no significant effect on proliferation of MIAPaCa-2 cells. These findings suggest that trypsin plays a role in the growth of PAR-2-positive pancreatic cancer cells and serves as a potent mitogen in vitro, functioning as a growth factor.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Protease-activated receptor-2 expression and the role of trypsin in cell proliferation in human pancreatic cancers

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Abstract

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