Selective up-regulation of tumor necrosis factor receptor I in tumor-bearing rats with cancer-related cachexia

  • Authors:
    • Maria G. Catalano
    • Nicoletta Fortunati
    • Katia Arena
    • Paola Costelli
    • Manuela Aragno
    • Oliviero Danni
    • Giuseppe Boccuzzi
  • View Affiliations

  • Published online on: August 1, 2003     https://doi.org/10.3892/ijo.23.2.429
  • Pages: 429-436
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Abstract

Tumor necrosis factor-α (TNF-α) and interleukin 6 (IL-6) are important mediators in cancer cachexia; however, the expression of these cytokines and their receptors in tumor-bearing animals is poorly characterized. We analyzed expression of TNF-α, IL-6, tumor necrosis factor (TNF-RI, TNF-RII) and interleukin 6 (IL-6R) receptors in the brain, kidney, spleen, liver, muscle, ascite tumors and serum, from Yoshida AH-130 hepatoma-bearing rats. TNF-α increased in the brain, spleen, liver, and muscle of cachectic animals; IL-6 increased in the liver and muscle. AH-130 cells expressed a good level of TNF-α; on the contrary, no TNF-α or IL-6 protein was detected in the serum of either tumor-bearing or control animals. TNF-RI mRNA was up-regulated in the spleen, liver and muscle of tumor-bearing rats. TNF-RI protein levels confirmed up-regulation in the spleen and liver, but failed to detect any increase in the muscle. Western blotting against TNF-RI revealed two bands of lower molecular weight in cachectic muscle, suggesting proteolysis involving TNF-RI. No significant increase of either TNF-RII or IL-6R was observed. This is the first demonstration of a selective up-regulation of TNF-RI in cancer cachexia and suggests that local production of TNF-α and IL-6 is a corner-stone in the induction/maintenance of this syndrome.

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August 2003
Volume 23 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Catalano MG, Fortunati N, Arena K, Costelli P, Aragno M, Danni O and Boccuzzi G: Selective up-regulation of tumor necrosis factor receptor I in tumor-bearing rats with cancer-related cachexia. Int J Oncol 23: 429-436, 2003.
APA
Catalano, M.G., Fortunati, N., Arena, K., Costelli, P., Aragno, M., Danni, O., & Boccuzzi, G. (2003). Selective up-regulation of tumor necrosis factor receptor I in tumor-bearing rats with cancer-related cachexia. International Journal of Oncology, 23, 429-436. https://doi.org/10.3892/ijo.23.2.429
MLA
Catalano, M. G., Fortunati, N., Arena, K., Costelli, P., Aragno, M., Danni, O., Boccuzzi, G."Selective up-regulation of tumor necrosis factor receptor I in tumor-bearing rats with cancer-related cachexia". International Journal of Oncology 23.2 (2003): 429-436.
Chicago
Catalano, M. G., Fortunati, N., Arena, K., Costelli, P., Aragno, M., Danni, O., Boccuzzi, G."Selective up-regulation of tumor necrosis factor receptor I in tumor-bearing rats with cancer-related cachexia". International Journal of Oncology 23, no. 2 (2003): 429-436. https://doi.org/10.3892/ijo.23.2.429