A commonly deleted region in ovarian cancer on chromosome 19p13.3, not including the OAZ1 gene

  • Authors:
    • Nozomu Yanaihara
    • Aikou Okamoto
    • Senya Matsufuji
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  • Published online on: September 1, 2003     https://doi.org/10.3892/ijo.23.3.567
  • Pages: 567-575
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Abstract

Antizyme is a negative regulator of cellular polyamines. The gene for antizyme (OAZ1) is mapped to 19p13.3, where frequent allelic imbalance (AI) is observed in ovarian cancer. The potential role of antizyme 1 in ovarian carcinogenesis was addressed in this study. Mutations of the OAZ1 gene, including the entire coding region and associated promoter region, were examined in 50 primary ovarian tumors and 8 ovarian cancer cell lines by PCR-SSCP and sequencing analyses. A missense mutation in exon 1 and a nucleotide change at the 3'-UTR were detected in an ovarian cancer cell line and its derivative cisplatin resistant cell line. No somatic mutation was detected in primary ovarian tumors, although 7 polymorphic sites were identified. AI of the OAZ1 gene was detected in 7 of 30 informative cases of primary ovarian cancer (23%). Subsequent multiplex fluorescent microsatellite analysis at 7 loci on 19p and at 4 loci on 19q in 50 primary ovarian tumors revealed a commonly deleted region, approximately 4.7 Mb, between the D19S424 and D19S884 loci on 19p13.3 in the vicinity of the OAZ1 locus. The most frequent AI was observed at D19S216 (50%). These results suggest that one or more tumor suppressor genes other than OAZ1 exist near the D19S216 locus on 19p13.3.

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September 2003
Volume 23 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Yanaihara N, Okamoto A and Matsufuji S: A commonly deleted region in ovarian cancer on chromosome 19p13.3, not including the OAZ1 gene. Int J Oncol 23: 567-575, 2003
APA
Yanaihara, N., Okamoto, A., & Matsufuji, S. (2003). A commonly deleted region in ovarian cancer on chromosome 19p13.3, not including the OAZ1 gene. International Journal of Oncology, 23, 567-575. https://doi.org/10.3892/ijo.23.3.567
MLA
Yanaihara, N., Okamoto, A., Matsufuji, S."A commonly deleted region in ovarian cancer on chromosome 19p13.3, not including the OAZ1 gene". International Journal of Oncology 23.3 (2003): 567-575.
Chicago
Yanaihara, N., Okamoto, A., Matsufuji, S."A commonly deleted region in ovarian cancer on chromosome 19p13.3, not including the OAZ1 gene". International Journal of Oncology 23, no. 3 (2003): 567-575. https://doi.org/10.3892/ijo.23.3.567