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International Journal of Oncology
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Print ISSN: 1019-6439 Online ISSN: 1791-2423
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September 2003 Volume 23 Issue 3

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Medicine International

An International Open Access Journal Devoted to General Medicine.

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September 2003 Volume 23 Issue 3

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Article

A hypoxic response induced in MatLyLu cells by cobalt chloride results in an enhanced angiogenic response by the chick chorioallantoic membrane

  • Authors:
    • T. Van Lieshout
    • J. Stanisz
    • V. Espiritu
    • M. Richardson
    • G. Singh

  • View Affiliations / Copyright

    Affiliations: Department of Pathology and Molecular Medicine, McMaster University, Hamilton Regional Cancer Centre, Hamilton, Ontario L8V 5C2, Canada
  • Pages: 745-750
    |
    Published online on: September 1, 2003
       https://doi.org/10.3892/ijo.23.3.745
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Abstract

Hypoxia is an important regulator of hypoxia inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) expression. Using Dunning rat prostate tumour cells (MatLyLu) we examined the induction of HIF-1α and VEGF by hypoxia and if the induction of these proteins would lead to angiogenesis in the chick chorioallantoic membrane (CAM). MatLyLu cells were exposed to 1% O2, in 5.25% CO2 and 94.75% N2, or treated with 100 mM CoCl2 to simulate hypoxia. Conditioned medium was analyzed using Western blots for HIF-1α or VEGF. VEGF levels were quantified using ELISA. Hypoxia significantly increased both HIF-1α and VEGF protein production. MatLyLu cells (1x106 viable cells in 50 µl of medium) grown under normoxic conditions induced in angiogenesis in the CAM, evaluated by the formation of a spoke wheel, and in cross sections by the number of blood vessels, following 5-day culture. This response was significantly increased using CoCl2-treated cells. Culture medium alone with or without CoCl2 was not angiogenic. These results provide direct evidence for the role of hypoxia in the induction of HIF-1α and VEGF which act as key angiogenic signals.

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Copy and paste a formatted citation
Spandidos Publications style
Van Lieshout T, Stanisz J, Espiritu V, Richardson M and Singh G: A hypoxic response induced in MatLyLu cells by cobalt chloride results in an enhanced angiogenic response by the chick chorioallantoic membrane. Int J Oncol 23: 745-750, 2003.
APA
Van Lieshout, T., Stanisz, J., Espiritu, V., Richardson, M., & Singh, G. (2003). A hypoxic response induced in MatLyLu cells by cobalt chloride results in an enhanced angiogenic response by the chick chorioallantoic membrane. International Journal of Oncology, 23, 745-750. https://doi.org/10.3892/ijo.23.3.745
MLA
Van Lieshout, T., Stanisz, J., Espiritu, V., Richardson, M., Singh, G."A hypoxic response induced in MatLyLu cells by cobalt chloride results in an enhanced angiogenic response by the chick chorioallantoic membrane". International Journal of Oncology 23.3 (2003): 745-750.
Chicago
Van Lieshout, T., Stanisz, J., Espiritu, V., Richardson, M., Singh, G."A hypoxic response induced in MatLyLu cells by cobalt chloride results in an enhanced angiogenic response by the chick chorioallantoic membrane". International Journal of Oncology 23, no. 3 (2003): 745-750. https://doi.org/10.3892/ijo.23.3.745
Copy and paste a formatted citation
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Spandidos Publications style
Van Lieshout T, Stanisz J, Espiritu V, Richardson M and Singh G: A hypoxic response induced in MatLyLu cells by cobalt chloride results in an enhanced angiogenic response by the chick chorioallantoic membrane. Int J Oncol 23: 745-750, 2003.
APA
Van Lieshout, T., Stanisz, J., Espiritu, V., Richardson, M., & Singh, G. (2003). A hypoxic response induced in MatLyLu cells by cobalt chloride results in an enhanced angiogenic response by the chick chorioallantoic membrane. International Journal of Oncology, 23, 745-750. https://doi.org/10.3892/ijo.23.3.745
MLA
Van Lieshout, T., Stanisz, J., Espiritu, V., Richardson, M., Singh, G."A hypoxic response induced in MatLyLu cells by cobalt chloride results in an enhanced angiogenic response by the chick chorioallantoic membrane". International Journal of Oncology 23.3 (2003): 745-750.
Chicago
Van Lieshout, T., Stanisz, J., Espiritu, V., Richardson, M., Singh, G."A hypoxic response induced in MatLyLu cells by cobalt chloride results in an enhanced angiogenic response by the chick chorioallantoic membrane". International Journal of Oncology 23, no. 3 (2003): 745-750. https://doi.org/10.3892/ijo.23.3.745
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