Characterisation and predictive value of epidermal growth factor receptor status using quantitative real-time PCR combined with immunohistochemistry on non-small cell lung cancer specimens

  • Authors:
    • Wolfgang Hilbe
    • Margit Dlaska
    • Stephan Dirnhofer
    • Hans-Christoph Duba
    • Wolfgang Eisterer
    • Frieda Oberwasserlechner
    • Andreas Mildner
    • Thomas Schmid
    • Karlheinz Ammann
    • Josef Thaler
    • Ewald Wöll
  • View Affiliations

  • Published online on: October 1, 2003     https://doi.org/10.3892/ijo.23.4.893
  • Pages: 893-899
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Abstract

The frequency of increased EGFR-mRNA expression was determined in 57 patients suffering from NSCLC by applying quantitative real-time PCR. The findings were correlated with clinical parameters and the immunohistochemical (IHC) markers EGFR, c-erbB-2, c-erbB-3, Ki-67 and p53 on cryostat sections. Of the patients 46% showed increased EGFR-mRNA, 35% revealed an increased IHC-EGFR expression; 16% of the patients showed a combined positivity and 35% a combined negativity when applying both methods, and 17 (30%) of the cases revealed increased EGFR-mRNA without IHC-EGFR expression. This subgroup was characterised by p53 coexpression and the highest frequency of deaths (35% vs. 20%) indicating a more aggressive tumour type. In contrast to IHC - where positivity was seen predominantly in squamous cell carcinomas (48% vs. 27%) - EGFR-mRNA expression was observed equally in both histological subtypes (48% vs. 43%). PCR-EGFR and IHC-EGFR tumour typing identifies different tumour characteristics with different clinical courses. Whether this combined typing could help to identify patients who respond to anti-EGFR therapies is worth further testing.

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October 2003
Volume 23 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Hilbe W, Dlaska M, Dirnhofer S, Duba H, Eisterer W, Oberwasserlechner F, Mildner A, Schmid T, Ammann K, Thaler J, Thaler J, et al: Characterisation and predictive value of epidermal growth factor receptor status using quantitative real-time PCR combined with immunohistochemistry on non-small cell lung cancer specimens. Int J Oncol 23: 893-899, 2003
APA
Hilbe, W., Dlaska, M., Dirnhofer, S., Duba, H., Eisterer, W., Oberwasserlechner, F. ... Wöll, E. (2003). Characterisation and predictive value of epidermal growth factor receptor status using quantitative real-time PCR combined with immunohistochemistry on non-small cell lung cancer specimens. International Journal of Oncology, 23, 893-899. https://doi.org/10.3892/ijo.23.4.893
MLA
Hilbe, W., Dlaska, M., Dirnhofer, S., Duba, H., Eisterer, W., Oberwasserlechner, F., Mildner, A., Schmid, T., Ammann, K., Thaler, J., Wöll, E."Characterisation and predictive value of epidermal growth factor receptor status using quantitative real-time PCR combined with immunohistochemistry on non-small cell lung cancer specimens". International Journal of Oncology 23.4 (2003): 893-899.
Chicago
Hilbe, W., Dlaska, M., Dirnhofer, S., Duba, H., Eisterer, W., Oberwasserlechner, F., Mildner, A., Schmid, T., Ammann, K., Thaler, J., Wöll, E."Characterisation and predictive value of epidermal growth factor receptor status using quantitative real-time PCR combined with immunohistochemistry on non-small cell lung cancer specimens". International Journal of Oncology 23, no. 4 (2003): 893-899. https://doi.org/10.3892/ijo.23.4.893